Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Biologically Active Materials Prepared for Several Minerals and Plants on the Growth of Rumen Microbes

무기물성 및 식물성 생리활성 물질이 반추위 미생물의 성장에 미치는 영향

  • Shin, Sung-Whan (Division of Applied Life Science(BK 21), GyeongSang National University) ;
  • Lee, Shin-Ja (Division of Applied Life Science(BK 21), GyeongSang National University) ;
  • Ok, Ji-Un (Division of Applied Life Science(BK 21), GyeongSang National University) ;
  • Lee, Sang-Min (Division of Applied Life Science(BK 21), GyeongSang National University) ;
  • Lim, Jung-Hwa (Division of Applied Life Science(BK 21), GyeongSang National University) ;
  • Kim, Kyoung-Hoon (National Institute of Animal Science, Animal Nutrition and Physiology Division) ;
  • Moon, Yea-Hwang (Department of Animal Sci. & Biotech, RAIC, Jinju National University) ;
  • Lee, Sung-Sill (Division of Applied Life Science(BK 21), GyeongSang National University)
  • 신성환 (경상대학교 응용생명과학부) ;
  • 이신자 (경상대학교 응용생명과학부) ;
  • 옥지운 (경상대학교 응용생명과학부) ;
  • 이상민 (경상대학교 응용생명과학부) ;
  • 임정화 (경상대학교 응용생명과학부) ;
  • 김경훈 (축산과학원 축산생명환경부 영양생리과) ;
  • 문여황 (진주산업대학교 동물생명과학과) ;
  • 이성실 (경상대학교 응용생명과학부)
  • Published : 2007.11.30
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Abstract

In order to know the effects of scoria, germanium, charcoal, ginger, stevia, and CLA(Conjugated Linoleic Acid) as biologically active materials on pathogenic microbes and rumen anaerobic microbes, the growth rate of pathogens (including Escherichia coli O157, Salmonella paratyphi, Listeria monocytogenes and Staphylococcus aureus) and in vitro lumen microbial growth, gas production, ammonia concentration, carboxymethyl-cellulase (CMCase) activity, and microbial populations were investigated. The growth of pathogenic microbes was inhibited by the supplement of 0.10% ginger. Ginger had powerful antimicrobial properties on all the pathogens used in this experiments. Additionally in the antibacterial assay by paper disc method, we could observe the clear zone of similar area with the positive control(antibiotics) for E. coli as applied with the 10% stevia or the 10% CLA only. The supplements of ginger, stevia and CLA in vitro rumen fermentation inhibited populations of rumen bacteria and protozoa. Particularly supplement of ginger resulted in remarkable reduction of the protozoa population, which means it might serve as a source inhibiting material of methane creation in the rumen.

본 연구는 무기물성 및 식물성 유래 생리활성물질로서 scoria, germanium, charcoal, 생강, stevia 및 CLA(conjugated linoleic acid)가 병원성 미생물 및 반추위 미생물에 대한 작용을 조사하기 위하여 수행되었다. 병원성 균으로서 Escherichia coli O157, Salmonella paratyphi, Listeria monocytogenes 및 Staphylococcus aureus에 적용하여 항균활성을 측정하고, in vitro 발효 시 반추위 미생물 성장률, 가스 생성량, 암모니아 농도, CMCase 활성 및 미생물의 수를 측정하였다. 병원성 미생물 배양액에 생강을 0.1% 첨가한 구에서만 항균활성이 나타났으나, paper disc법에 의한 항균활성 시험에서는 stevia 10%첨가구와 CLA 10% 첨가구에서 E. coli에 대해서 항생제 첨가구인 positive control 구와 비슷한 크기의 clear zone을 형성하였다. in vitro 반추위 미생물 발효시험에서는 생강, stevia 및 CLA가 반추위 박테리아와 프로토조아의 증식을 억제하는 것으로 나타났는데, 특히 생강 첨가구의 경우 메탄 생성균의 서식지로 알려져 있는 프로토조아를 크게 억제함으로써 메탄생성 억제제로서 개발 가능성이 있는 것으로 사료된다.

Keywords

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