Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
권호 표지

Changes in Sex Steroid Hormones and Ovarian Development during Artificial Maturation of Female Eel, Anguilla japonica

  • Kim, Dae-Jung (Aquaculture Research Team, Headquarters for Aquaculture, National Fisheries Research and Development Institute (NFRDI)) ;
  • Bae, Jun-Young (Aquaculture Research Team, Headquarters for Aquaculture, National Fisheries Research and Development Institute (NFRDI)) ;
  • Kim, Eung-Oh (Inland Aquaculture Research Institute NFRDI)
  • Published : 2007.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The present study demonstrates the changes in body weight (BW) and plasma sex steroid hormone profiles during artificial maturation induced by human chorionic gonadotropin (HCG) or salmon pituitary extract (SPE) injections in cultured eel, Anguilla japonica, kept in seawater for 3 months. In the weekly SPE-injected female group, BW was relatively stable during vitellogenesis. Following induction of vitellogenesis, females exhibited a rapid increase of BW, and the oocytes were observed to be in the migratory nucleus stage at the end of the experiment. Plasma testosterone (T) and $estradiol-17{\beta}$ ($E_2$) levels increased slightly during vitellogenesis and peaked at an average of 5.82 ng/mL and 4.76 ng/mL, respectively, at the end of the experiment. In the weekly control and HCG-injected female groups, BW slowly decreased during the experimental period, and the oocytes of the two groups were observed to be at the primary yolk globule stage. In the weekly HCG-injected female group, plasma T and $E_2$ levels increased slightly during vitellogenesis and decreased afterward. In the control female group, however, plasma T and $E_2$ levels were not altered during the experimental period. Furthermore, plasma $17{\alpha},20{\beta}-dihydroxy-4-pregnen-3-one$ (DHP) was not detected in all experimental groups. Fertility and hatching rates of SPE-injected females were significantly higher in those that ovulated 15 h after DHP injection than 18 h. These results indicate that long rearing in seawater increases responsiveness to SPE in ovarian maturation of the Japanese eel, resulting in shortened period from completion of vitellogenesis by sex steroid hormone production.

Keywords

References

  1. Boetius I and Boetius J (1980) Experimental maturation of female silver eels, Anguilla anguilla. Estimates of fecundity and energy reserves for migration and spawning. Dana 1: 1-28
  2. Chiba H, Iwatsuki K, Hayami K, Hara A and Yamauchi K (1994) Changes in serum steroid hormones and vitellogenin levels in cultured female European eels (Anguilla anguilla) during artificially induced ovarian development. J World Aquacult Soc 25: 553-560 https://doi.org/10.1111/j.1749-7345.1994.tb00824.x
  3. Hirose K, Hirano T and Ishida R (1974) Effects of salmon gonadotropin on ovulation in the ayu, Plecoglossus altiuelis with special reference to water balance. Comp Biochem Physiol 47A: 283-289
  4. Hirose K (1976) Endocrine control of ovulation in medaka (Oryzias latipes) and ayu (Plecoglossus altiuelis). J Fish Res Bd Can 33: 989-999 https://doi.org/10.1139/f76-125
  5. Hirose K (1992) Induced spawning of Japanese eel with LHRH-A copolymer pellet. NOAA Tech Rep NMFS 106: 43-48
  6. Ishida R, Hirose K and Donaldson EM (1972) Induction of ovulation in ayu, Plecoglossus altivelis, with salmon pituitary gonadotropin. Bull Jpn Soc Sci Fish 38: 1007-1012 https://doi.org/10.2331/suisan.38.1007
  7. Ijiri S, Kazeto Y, Takeda N, Chiba H, Adachi S and Yamauchi K (1995) Changes in serum steroid hormones and steroidogenic ability of ovarian follicles during artificial maturation of cultivated Japanese eel, Anguilla japonica. Aquaculture 135: 3-16 https://doi.org/10.1016/0044-8486(96)81292-0
  8. Ijiri S, Takaki T, Takeda N, Tachiki H, Adachi S and Yamauchi K (1998) Pretreatment reproductive stage and oocyte development induced by salmon pituitary homogenate in the Japanese eel, Anguilla japonica. Fish Sci 64: 531-537 https://doi.org/10.2331/fishsci.64.531
  9. Kagawa H, Iinuma N, Tanaka H, Ohta H and Okuzawa K (1998) Effects of rearing period in seawater on induced maturation in female Japanese eel, Anguilla japonica. Fish Sci 64: 77-82 https://doi.org/10.2331/fishsci.64.77
  10. Kim DJ, Kim EO, Park MW, Cho YC and Lim SO (2006a) Plasma sex steroid hormone profiles in artificially maturing wild eel, Anguilla japonica. J Aquacult 19:267-274
  11. im EO, Bae JY, Lim SG, Son MH, Park MW, Park MS, Cho YC and Kim DJ (2006b) Plasma sex steroid hormone profiles and testicular development in artificially maturing cultured male eel, Anguilla japonica. J Kor Fish Soc 39: 466-471 https://doi.org/10.5657/kfas.2006.39.6.466
  12. Ko HY, Park WD, Kim DJ, Kobayashi M and Sohn YC (2007) Biological activities of recombinant Manchurian trout FSH and LH: their receptor specificity, steroidogenic and vitellogenic potencies. J Molecular Endocrinol 38: 99-111 https://doi.org/10.1677/jme.1.02163
  13. Matsubara H, Lokman PM, Kazeto Y, Adachi S and Yamauchi K (2005) Serum steroid profiles in artificially maturing female Japanese eel, Anguilla japonica. Aquaculture 243: 393-402 https://doi.org/10.1016/j.aquaculture.2004.10.018
  14. Ochiai A, Umeda S and Ota H (1972) On the eggs of Japanese eel and induction of maturation by hormone injection. Jpn J Ichthyol 19: 312-316
  15. Ohta H, Kagawa H, Tanaka H, Okuzawa K and Hirose K (1996) Changes in fertilization and hatching rates with time after ovulation induced by 17,20 ${\beta}$-dihydroxy-4-pregnen-3-one in the Japanese eel, Anguilla japonica. Aquaculture 139: 291-301 https://doi.org/10.1016/0044-8486(95)01167-6
  16. Oka PH (1979) Studies on the artificial maturation in the Japanese eel, Anguilla japonica. On the relation between the changes in body weight and the oogenesis during the course of maturation. Bull Shizuoka Pref Fish Exp Stn 13: 101-111
  17. Sato N, Kawazoe I, Suzuki Y and Aida K (2000) Adequate intervals of sGTH administration to induce vitellogenesis, and induction of ovulation by simultaneous administration of sGTH and 17${\alpha}$-hydroxyprogesterone in the Japanese eel. Fish Sci 66: 644-654 https://doi.org/10.1046/j.1444-2906.2000.00099.x
  18. Sato N, Kawazoe I, Suzuki Y and Aida K (2003) Induction of vitellogenesis. In: Eel Biology (ed), Aida K, Tsukamoto K and Yamauchi K, Springer-Verlag, Tokyo, pp 387-399
  19. Satoh H, Yamamori Y and Hibiya T (1992) Induced spawning of the Japanese eel. Nippon Suisan Gakkaishi 58: 825-832 https://doi.org/10.2331/suisan.58.825
  20. Shehadeh Z and Ellis IN (1970) Induced spawning ofthe striped mullet, Mugil cephalas. J Fish BioI 2: 355-360 https://doi.org/10.1111/j.1095-8649.1970.tb03293.x
  21. Sugimoto Y, Takeuchi Y, Yamauchi K and Takahashi H (1976) Induced maturation of female Japanese eels (Anguilla japonica) by administration of salmon pituitaries, with notes on changes of oil droplets in eggs of matured eels. Bull Fac Fish Hokkaido Univ 27: 107-120
  22. Tsukamoto K, Lee TW and Fricke H (2003) Spawning area of the Japanese eel. In: Eel Biology (ed), Aida K, Tsukamoto K and Yamauchi K, Springer-Verlag, Tokyo, pp 121-140
  23. Yamamoto K, Hiroi O, Hirano T and Morioka T (1972) Artificial maturation of cultivated male Japanese eel by synahorin injection. Bull Jpn Sac Sci Fish 38: 1083-1090 https://doi.org/10.2331/suisan.38.1083
  24. Yamamoto K and Yamauchi K (1974) Sexual maturation of Japanese eel and production of eel larvae in the aquarium. Nature 251: 220-221 https://doi.org/10.1038/251220a0
  25. Yamamoto K, Morioka T, Hiroi O and Omori M (1974) Artificial maturation of female Japanese eels by the injection of salmonid pituitary. Nippon Suisan Gakkaishi 40: 1-7 https://doi.org/10.2331/suisan.40.1
  26. Yamauchi K, Nakamura M, Takahashi Hand Takano K (1976) Cultivation of larvae of Japanese eel. Nature 263: 412