Changes of Microorganisms During Fresh-Cut Cabbage Processing: Focusing on the Changes of Air-Borne Microorganisms

신선편이 양배추 제조공정 단계별 미생물 변화: 공기 중 미생물 변화를 중심으로

  • Seo, Jung-Eun (Food Safety Research Center, Korea Food Research Institute) ;
  • Lee, Jong-Kung (Food Safety Research Center, Korea Food Research Institute) ;
  • Oh, Se-Wook (Food Safety Research Center, Korea Food Research Institute) ;
  • Koo, Min-Seon (Food Safety Research Center, Korea Food Research Institute) ;
  • Kim, Young-Ho (Food Safety Research Center, Korea Food Research Institute) ;
  • Kim, Yun-Ji (Food Safety Research Center, Korea Food Research Institute)
  • 서정은 (한국식품연구원 안전성연구단) ;
  • 이종경 (한국식품연구원 안전성연구단) ;
  • 오세욱 (한국식품연구원 안전성연구단) ;
  • 구민선 (한국식품연구원 안전성연구단) ;
  • 김영호 (한국식품연구원 안전성연구단) ;
  • 김윤지 (한국식품연구원 안전성연구단)
  • Published : 2007.12.31

Abstract

To evaluate effects of airborne microorganisms in fresh cut processing plant, microorganisms in air, equipments, raw material, water and final product were isolated and identified using Vitek (R)2 compact system. Airborne microorganisms were isolated from 1000L air using air sampler for floating microorganisms and plate count agar for falling microorganisms. And contaminated microorganisms of equipment, water, and product were isolated from plate count agar plate. Total plate counts for floating and falling, raw material, equipments and final product were $10^2-10^3CFU/m^3,{\sim}10^1CFU/plate,\;10^3CFU/g,{\sim}10^4CFU/cm^2\;and\;10^4CFU/g$, respectively. From the result of isolated microorganism identification from raw material to final product, airborne microorganisms could affect the flora of final product.

작업장 내 공기오염의 중요성을 평가하기 위하여 신선편이채소 작업장 내 실내 공기와 제품에서 검출되는 미생물 종류 및 수준을 조사하고 양배추 생산작업을 대상으로 관련된 작업공간의 공기 미생물, 작업설비 및 세척수, 원료 및 제품에 대하여 미생물 실험을 실시하였다. 실내 공기 중의 미생물은 air sampler를 이용하여 1000 L의 공기에서 부유균을, plate count agar를 이용하여 낙하균을 각각 8 회 포집하여 분리하였으며 그 외 장비와 제품에 대한 미생물은 총균수 측정 후 colony를 분리하였으며 분리된 균은 Vitek (R)2 compact system으로 동정하였다. 부유균과 낙하균의 총균수준은 각각 $10^2-10^3CFU/m^3,{\sim}10^1CFU/plate$, 또한 원료, 생산라인 및 최종제품에서 검출된 총균수준은 각각 $10^3CFU/g,{\sim}10^4CFU/cm^2,\;10^4CFU/g$수준이었다. 동정결과 생산과정의 여러 검체에서 공기미생물과 같은 균종이 검출되어 공기 미생물이 제품의 오염에 영향을 줄 수 있는 가능성을 보여주었다.

Keywords

References

  1. Kim, D.-M. and Hong, S.I.: Current status and prospect of fresh-cut produce in Korea, Food Preserv. Proc. Ind., 3, 18-22 (2004)
  2. Kim, B.-S.: Equipments and facilities to process high quality of fresh-cut produce, Food Preserv. Proc. Ind., 4, 41-61 (2005)
  3. Kim, K.-H.: Consumers' demand for fresh cut produce, . Food Preserv. Proc. Ind., 4, 2-7 (2005)
  4. Park, Y.-J., Hwang, T.Y., and Moon, K.D.: Plan of establishment for minimal processing fruits & vegetables, . Food Preserv. Proc. Ind., 4, 8-17 (2005)
  5. Hwang, T.-Y. and Moon, K.D.: Technical trend & prospect of minimal processing fruits & vegetables industry, Food Sci. Ind., 38, 120-130 (2005)
  6. Shlech, W.F., Lavigne, P.M., Bortolussi, R.A., Allen, A.C., Haldan, E.V., Wort, A.J., and Hightower, A.W.: Epidermic listeriosis evidence for transmission by food, N. Engl. J. Med., 308, 203-206 (1983) https://doi.org/10.1056/NEJM198301273080407
  7. KIm, C., Hung, Y.C., and Brackett, R.E.: Efficacy of electrolyzed oxidizing (EO) and chemically modified water on different types of foodborne pathogens. Int. J. Food Microbiol., 61, 199-207 (2005)
  8. Korea Food and Drug Administration. Trend of safety management for fresh-cut produce in USA. http://www.kfda.go.kr (2007)
  9. Kim, J.K.: Techniques to establish safety of fresh-cut fruits and vegetables. Food Preserv. Proc. Ind., 4, 18-25 (2005)
  10. Food and Drug Administration and Milk Industry Foundation International Ice Cream Association. Recommended guidelines for controlling environmental contamination in dairy plants. Dairy Food Sanit. 8, 52-56 (1988)
  11. Kang, Y.J. and Frank, J.F.: Biological aerosols: A review of airbone contamination and its measurement in dairy processing plants, J. Food Prot., 52, 512-524 (1989) https://doi.org/10.4315/0362-028X-52.7.512
  12. Kang, Y.J., and Frank, J.F.: Characteristics of biological aerosols in dairy processing plants, J. Dairy Sci., 73, 621-626 (1990) https://doi.org/10.3168/jds.S0022-0302(90)78712-7
  13. Chung, Y.H., Hong, J.B., and Chang, Y.H.: A Study on the microbial air pollution of urban living and indoor environment, Kor. J. Env. Hlth. Soc., 27, 1-9 (2001)