Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Adherence Rate of Listeria monocytogenes Contaminated from Pork Meat During Pork Meat Processing

포장돈육 가공공정 중 Listeria monocytogenes에 오염된 돈육으로부터 오염 부착율

  • Kim, Seong-Jo (Department of Food Industry Development, Korea Health Industry Development Institute) ;
  • Bahk, Gyung-Jin (Department of Food Industry Development, Korea Health Industry Development Institute) ;
  • Oh, Deog-Hwan (Department of Food Science and Biotechnology, College of Bioscience and Biotechnology, Kangwon National University)
  • 김성조 (한국보건산업진흥원) ;
  • 박경진 (한국보건산업진흥원) ;
  • 오덕환 (강원대학교 생명공학부 식품생명공학)
  • Published : 2007.12.31
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Abstract

This study was done to determine the adherence rate of Listeria monocytogenes(LM) from pork meat to pork meat, conveyer belt and stainless steel, and cutting board at $10^{\circ}C$ for given times (2, 4, 24 hours). The adherence rates of ATCC and wild type LM from pork meat to red meat or fat meat after 2 hour contact time were approximately 65-79%, but 100% adherence rates of both LM after 6 hours were observed. The adherence rate of ATCC LM from pork meat to conveyer belt and stainless steel after 24 hours was 0.15%, while the adherence rate of wild type LM was rapidly increased from 4.34 after 2 hours to 28.6% after 24 hours. Also, The adherence rate of ATCC LM from pork meat to stainless steel after 24 hours ranged from 2.63% after 2 hours to 0.39% after 24 hours, while the adherence rate of wild type LM ranged from 0% after 2 hours to 24.4% after 24 hours. In the meantime, no adherence rate of both LM from pork meat to cutting board was observed. This results suggest that the adherence rate of LM from pork meat to pork meat or food contact surfaces was significantly affected by contact time and wild type LM showed much higher adherence rate to pork meat or food contact surfaces than ATCC LM.

L. monocytogenes 표준균주와 분리균주에 오염된 돈육을 $10^{\circ}C$에서 다른 돈육(삼겹살, 안심), 컨베이어벨트와 스테인리스 스틸, 도마에 일정시간(2, 4, 24시간)동안 접촉시킨 후 부착율을 조사하였다. LM 표준균주와 분리균주에 오염된 돈육으로부터 삼겹살과 안심에 2시간 접촉 시 부착율은 약 65-79%를 나타내었으나 6시간 이후부터는 두균 모두 100%의 부착을 보였다. 돈육으로부터 컨베이어 벨트로의 부착율은 표준균주의 경우 24시간 후 0.19%의 부착율을 나타내었으나 분리균주는 2시간 접촉시 4.34%에서 24시간 후 28.6%로 급격히 증가하였다. 또한, 돈육으로부터 스테인레스 스틸로의 부착율은 표준균주는 2시간 후 2.63%의 부착율을 나타내었으나 24시간 후에는 0.39%로 감소하였고, 분리균주는 2시간 후 전혀 접촉이 없었으나 24시간 후에는 24.4%로 현저하게 증가하였다. 반면에 돈육으로부터 도마로의 부착율은 표준균주 및 분리균주 모두 전혀 부착하지 않았다. 본 연구결과, L. monocytogenes균에 오염된 돈육에서 다른 돈육 또는 식품접촉면으로의 부착율은 접촉시간에 따라 영향이 컸으며 분리균주가 표준균주에 비하여 현저하게 높은 부착율을 나타내었다.

Keywords

References

  1. URL: http://koreapork.or.kr/index.htm 대한양돈협회
  2. URL: http://www.kfda.go.kr/korea/food/virus5.html
  3. Kim, N.H. and Tak, R.B. : Contamination of Salmonella spp. In Chickens Slaughtered in Taegu Area. Kor. J, Vet. Publ. Hlth, 22(4), 365-372 (1998)
  4. Sammarco, M.A., Ripabelli, G., Ruberto, A., Iannitto, G. and Grasso, G.M. : Prevalence of Salmonellae, Listeriae, Yersiniae in the Slaughterhouse environment and on work surface, equipment, and workers. J. Food Prot., 60 367-371 (1997) https://doi.org/10.4315/0362-028X-60.4.367
  5. Borch, E., Nesbakken, T. and Christensen, H. : Hazard identification in swin slaughter with respect to foodborne bacteria. Int. J. Food Microbiol. 30, 9-25 (1996) https://doi.org/10.1016/0168-1605(96)00988-9
  6. 정명섭 : 위생적인 돈육생산 및 수출을 위한 HACCP 제도 도입을 위한 workshop-국제환경 변화에 따른 HACCP 제도의 적용. 한국축산식품학회 0(0), 16-83 (1997)
  7. Montville, R., Chen, Y.H. and Schaffner, D.W. : Glove barriers to bacterial cross-contamination between hands to food. J. Food Prot. 64(2), 845-849 (2001) https://doi.org/10.4315/0362-028X-64.6.845
  8. Maturin, L.J. and Peeler, J.T. : in Bacteriogical Analytical Manual, 8th Ed., Rev. A, G. J. Jackson (Ed.), AOAC INTRENATIONAL, Gaithersburg, MD, 301-310 (1998)
  9. SPSS. Statistical PaCkage for Social Sciences for Windows. Rel. 10.0. SPSS Inc., Chicago, IL, USA (1999)
  10. Jung CY, Choi LG. SPSSWIN for Statistics Analysis, Ver. 10.0, 4th ed. Muyok Publishing Co., Seoul, Korea, 276-283 (2002)
  11. Dickson, J.S. : Transfer of Listeria monocytogenes and Salmonella typhimurium Between Beef Tissue Surfaces, J. Food Prot., 53(1), 51-55 (1990) https://doi.org/10.4315/0362-028X-53.1.51
  12. Midelet, G. and Carpentier, B. : Transfer of microorganisms, including Listeria monocytogenes, from various materials to beef. Applied and environmental microbiology, 68(8), 4015-4024 (2002) https://doi.org/10.1128/AEM.68.8.4015-4024.2002
  13. Norwood. D.E. : The differential adherence capabilities of two Listeria monocytogenes strains in monoculture and multispecies biofilms as a function of temperature. Letters in Applied Microbiology. 33(4), 323-324 (2001)
  14. Akier Assanta Mafu, Denis Roy, Jacques Goulet, and Pierre Magny : adherence of Listeria monocytogenes to Stainless steel, Glass, Polypropylene, and Rubber Surfaces After Short Contact Times. J. Food Prot., 53(9) 742-746 (1990) https://doi.org/10.4315/0362-028X-53.9.742
  15. Yun, H.J., Kho, Y.L., and Lee, Y.W. : Studies on Growth and Decontamination of Listeria monocytogenes Attached to Food Contact Surface Materials, Kor. J, Env. Hlth. 27(1), 75-82 (2001)