Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Effects of Supplemental Asterias amurensis Extract in the Experimental Diets on Growth, Blood Chemistry and Superoxide Production of Kidney Phagocytes of Sebastes schlegeli

불가사리(Asterias amurensis) 추출물을 첨가한 사료의 급이가 조피볼락(Sebastes schlegeli)의 성장, 혈액성상 및 식세포 활성산소 생산에 미치는 효과

  • Park, Hee-Yeon (Biotechnology Research Center, National Fisheries Research & Development Institute) ;
  • Lim, Chi-Won (Biotechnology Research Center, National Fisheries Research & Development Institute) ;
  • Kim, Yeon-Kye (Biotechnology Research Center, National Fisheries Research & Development Institute) ;
  • Choi, Tae-Jin (Biotechnology Research Center, National Fisheries Research & Development Institute) ;
  • Yoon, Ho-Dong (Biotechnology Research Center, National Fisheries Research & Development Institute) ;
  • Lee, Ka-Jung (Biotechnology Research Center, National Fisheries Research & Development Institute) ;
  • Seo, Yeon-Kyung (Biotechnology Research Center, National Fisheries Research & Development Institute) ;
  • Kim, Ji-Yeong (Biotechnology Research Center, National Fisheries Research & Development Institute) ;
  • Park, Ki-Eui (Biotechnology Research Center, National Fisheries Research & Development Institute)
  • 박희연 (국립수산과학원 생명공학연구소) ;
  • 임치원 (국립수산과학원 생명공학연구소) ;
  • 김연계 (국립수산과학원 생명공학연구소) ;
  • 최태진 (국립수산과학원 생명공학연구소) ;
  • 윤호동 (국립수산과학원 생명공학연구소) ;
  • 이가정 (국립수산과학원 생명공학연구소) ;
  • 서연경 (국립수산과학원 생명공학연구소) ;
  • 김지영 (국립수산과학원 생명공학연구소) ;
  • 박기의 (국립수산과학원 생명공학연구소)
  • Published : 2007.12.31
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Abstract

This study was conducted to examine the effects of Asterias amurensis prethanol extract on growth performance, serum traits, and superoxide production of phagocytes in Sebastes schlegeli. The effects of Asterias amurensis extract on growth performance, specific growth rate (SGR), feed concentration ratio (FCR), coefficient of fatness (CF), and survival rate (SR) of fish fed diets containing various concentrations of Asterias amurensis extract were measured. There were no significant differences in SGR, FCR, CF, and SR among the experimental groups. This result was produced because experimental diets were coated to prevent repellent action of fish. To investigate the effects of Asterias amurensis extract on the metabolism, the contents of glucose, glutamic oxaloacetic transaminase (GOT), and total cholesterol in serum were measured. The contents of glucose and total cholesterol in serum increased dose-dependently and serum GOT content showed no significant difference among the experimental groups, suggesting that Asterias amurensis extract was non-toxic material. To confirm the effects of Asterias amurensis extract on the immune system of fish, superoxide production of phagocytes was measured. Asterias amurensis extract caused a dose-dependent increase of superoxide production of phagocytes. When considering these results, Asterias amurensis extract could be utilized as an additive to augment immune function in diets.

수산 해적생물인 불가사리의 주정추출물을 농도별로 사료에 첨가하여 조피볼락의 성장, 혈액성상 및 식세포 활성산소 생산에 미치는 효과를 조사하였다. 불가사리추출물의 함량이 다른 사료를 급여한 조피볼락의 1일성장율(SGR), 사료계수(FCR), 비만도(CF), 생존율(SR)을 비교한 결과, 실험군별 유의적인 차이를 보이지 않았다. 이는 불가사리추출물에 의한 먹이 기피현상을 방지하기 위해 사료를 코팅을 하였기 때문인 것으로 사료된다. 불가사리추출물이 조피볼락의 체내 대사계에 미치는 영향을 알아보기 위해 혈청 중의 glucose, total cholesterol의 함량과 GOT를 측정한 결과, glucose와 total cholesterol의 함량은 불가사리추출물의 함량이 증가할수록 높아지는 경향을 보였다. 한편, 혈청 GOT는 실험군별로 유의적인 차이를 보이지 않았으며 이는 불가사리추출물이 독성이 거의 없기 때문인 것으로 추정된다. 불가사리추출물 첨가사료의 급이가 조피볼락의 면역계에 미치는 영향을 알아보기 위하여 식세포의 활성산소 생산량을 측정한 결과, 불가사리추출물의 첨가에 따라 농도 의존적으로 증가하였다. 이상의 실험결과에서 불가사리추출물은 면역증강 기능성 사료첨가제로 활용할 수 있을 것으로 사료되었다.

Keywords

References

  1. Lee, S. M., Park, C. S. and Bang, J. C. (2002) Dietary protein requirement of young Japanese flounder Paralichthys olivaceus fed isocaloric diets. Fisheries Science 68, 158-164 https://doi.org/10.1046/j.1444-2906.2002.00402.x
  2. Aoki, T., Kanazawa, T. and Kitao, T. (1985) Epidemiological surveillance of drug resistant Vibrio anguillarum strains. Fish Pathol. 29, 199-208
  3. Kwon, M. G., Kim, Y. C., Shon, Y. C. and Park, S. I. (1999) The dietary supplementing effects of Kugija, Lycium chinense, on immune responses of Nile tilapia, Oreochromis niloticus, to Edwardsiella tarda. J. Fish Pathol. 12, 73-81
  4. Nakagawa, H., Kasahara, S., Uno, E., Minami, T. and Akira, K. (1981) Effect of Chlorella-extract supplement in diet on resisting power against disease of cultured ayu. Aquaculture 29, 109-116 https://doi.org/10.1016/0044-8486(82)90038-2
  5. Satoh, K. I., Nakagawa, H. and Kasahara, S. (1987) Effect of Ulva meal supplementation on disease resistance of red sea bream. Nippon Suisan Gakkaishi 53, 1115-1120 https://doi.org/10.2331/suisan.53.1115
  6. Matuso, K. and Miyazano, I. (1993) The influence of long-term administration of peptidoglycan on disease resistance and growth of juvenile rainbow trout. Nippon Suisan Gakkashi 59, 1377-1379 https://doi.org/10.2331/suisan.59.1377
  7. Jang, S. I., Jo, J. Y. and Lee, J. S. (1992) Effects of vitamins and glycyrrhizin added to oxidized diets on the growth and on the resistance to Edwardsiella infection of Nile tilapia, Oreochromis niloticus. J. Aquacult. 5, 143-155
  8. Yasumoto, T., Watanabe, T. and Hashimoto, Y. (1964) Physiological activities of starfish saponin. Nippon Suisan Gakkaishi 30, 357-364 https://doi.org/10.2331/suisan.30.357
  9. Hashimoto, Y. and Yasumoto, T. (1960) Confirmation of saponin as a toxic principle of starfish. Nippon Suisan Gakkaishi 26, 1132-1138 https://doi.org/10.2331/suisan.26.1132
  10. Higashi, H., Murayama, S., Yanase, M. and Tabei, K. (1955) Studied on utilization of worthless marine animals for feed-I. Production of starfish solubles for feed. Nippon Suisan Gakkaishi 21, 271-279 https://doi.org/10.2331/suisan.21.271
  11. Kim, J. H. (1998) Effects of Obosan (dietary herbs) on growth, body compositions, hematological characteristics and textural properties in all-female olive flounder, Paralichthys olivaceus. Ph. D. Thesis, Pukyung National University, Busan
  12. Rubin, E. and Lieber, C. S. (1974) Fatty liver, alcoholic hepatitis and cirrhosis produced by alcohol in primate. N. Engl. J. Med. 290, 128-135 https://doi.org/10.1056/NEJM197401172900303
  13. Lieber, C. S., DeCarli, L. and Rubin, E. (1975) Sequential production of fatty liver hepatitis, and cirrhosis in sub-human primates fed ethanol with adequate. Proc. Natl. Acad. Sci. USA 72, 437-441
  14. Allgen, L. G., Izikowitz, S., Nauckhoff, B., Ordell, I. B. and Salum, I. (1958) Serum diphosphopyridine nucleotide linked enzymes in delirium tremens and allied conditions. Science 128, 304-305
  15. Bang, N. U., Iverson, K., Jagt, T. and Madson, S. (1958) Serum glutamic oxaloacetic transaminase activity in acute and chronic alcoholism. JAMA 168, 156
  16. Ellis, A. E. (2000) Imminity to bacteria in fish. Fish Shellfish Immunol. 9, 291-308 https://doi.org/10.1006/fsim.1998.0192
  17. Park, K. U., Wee, J. J., Kim, J. Y., Jeong, C. H., Kang, K. S., Cho, Y. S. and Seo, K. I. (2005) Anticancer and immunoactivities of edible crude saponin from soybean cake. J. Koean Soc. Food Sci. Nutr. 34, 1509-1513 https://doi.org/10.3746/jkfn.2005.34.10.1509
  18. Liener, I. E., Sharon, N. and Goldstein, I. J. (1986) Biological properties of Lectins. In The Lectin, pp. 266, Academic Press, New York
  19. Jung, S. H., Shon, Y. C. and Kim, Y. C. (2001) In vitro effect of water extract of medical herbs on antimicrobial activity against fish pathogenic bacteria and superoxide production of kidney phagocytes in olive flounder, Paralichthys olivaceus. J. Fish Pathol. 14, 3-10
  20. Park, S. U., Kwon, M. G., Lee, Y. H., Kim, K. D., Shin, I. S. and Lee, S. M. (2003) Effects of supplemental Undaria, Obosan and Wasabi in the experimental diets on growth, body composition, blood chemistry and non-specific immune response of juvendile flounder, Paralichthys olivaceus. J. Aquacult. 16, 210-215