Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Variation of the Sensitivity of Botrytis cinerea causing Ginseng Grey Mold to Fungicides Inhibiting β Assembly

β 단백질의 중합을 억제하는 살균제에 대한 인삼 잿빛곰팡이병균의 감수성 변화

  • Kim, Joo-Hyung (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Min, Ji-Young (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Baek, Young-Soon (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Bae, Yeoung-Seuk (Ginseng & Medicinal Plants Research Institute, National Institute of Crop Science(NICS), RDA) ;
  • Kim, Heung-Tae (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University)
  • 김주형 (충북대학교 농업생명환경대학 응용생명환경학부 식물의학) ;
  • 민지영 (충북대학교 농업생명환경대학 응용생명환경학부 식물의학) ;
  • 백영순 (충북대학교 농업생명환경대학 응용생명환경학부 식물의학) ;
  • 배영석 (농촌진흥청 작물과학원 인삼약초연구소) ;
  • 김흥태 (충북대학교 농업생명환경대학 응용생명환경학부 식물의학)
  • Published : 2007.12.01
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Abstract

In this experiment, 236 isolates of Botrytis cinerea isolated from the lesions of ginseng grey mold in 2005 and 2006 were examined for their sensitivity to fungicide inhibiting ${\beta}-tubulin$ assembly. The baselines of fungicide resistance were determined as 10.0 and $0.2{\mu}g/ml$ of $EC_{50}$ values for carbendazim and the mixture of carbendazim and diethofencarb, respectively. The ratios of isolates resistant to carbendazim in 2005 and 2006 was investigated to be 87.6 and 96.6%, respectively. In the case of the mixture of carbendazim and diethofencarb, the ratio of the resistant isolates was 23.6% for 2005 and 24.5% for 2006. The ratio of the resistant isolates to the mixed fungicide was fluctuated according to regions where isolates of B. cinerea were obtained. In Yeoncheon of Gyeonggi Province, 4.3% of the isolates used in the experiment was resistant in 2005 and no resistant isolate was obtained in 2006. Among 5 regions, the ratio of resistant isolates was the highest as 70.0% in Yecheon of Gyeongbuk Province.

2005년과 2006년에 인삼 잿빛곰팡이병균을 각각 89개와 147개씩을 분리하여 실험에 사용하였다. Carbendazim은 $EC_{50}$값이 $10.0{\mu}g/ml$ 이상을, carbendazim/diethofencarb 합제는 $0.2{\mu}g/ml$ 이상을 저항성 검정의 기준으로 하였다. 이 기준을 가지고서 두 종류의 살균제에 대한 저항성 균주의 분리 빈도를 조사하면 carbendazim의 경우, 2005년에 87.6%, 2006년에는 96.6%로 나타나, 포장에서 분리되는 대부분의 Botrytis cinerea가 저항성균인 것으로 밝혀졌다. Carbendazim/diethofencarb 합제의 경우에는 저항성 균주의 분리빈도는 2005년과 2006년에 각각 23.6%와 24.5%로 나타났다. Carbendazim/diethofencarb 합제는 지역에 따라서 저항성균의 분리 빈도가 크게 달랐다. 경기도 연천 지역의 경우 저항성 균주의 분리빈도가 2005년에는 4.3%였고, 2006년에는 저항성 균주가 전혀 분리되지 않았다. 이에 비하여 다른 지역의 경우 저항성 균주의 빈도가 크게 높았는데, 2005년에는 경북 봉화와 예천 그리고 경기도 파주의 저항성 균주의 빈도가 각각 35.5%, 70.0% 및 40.0%였고, 2006년에는 전북 정읍의 저항성 균주의 빈도가 47.1%였다. 따라서 carbendazim/diethofencarb 합제에 대한 저항성 검정이 포장에서 지속적으로 수행되어야 한다.

Keywords

References

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