Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Antioxidant Effect of Garlic Supplement against Exercise-Induced Oxidative Stress in Rats

운동으로 유발된 산화 스트레스와 마늘의 항산화 작용

  • Yoon, Gun-Ae (Department of Food and Nutrition, Dongeui University)
  • 윤군애 (동의대학교 생활과학대학 식품영양학과)
  • Published : 2007.12.30
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Abstract

This study was to investigate lipid peroxidation, antioxidant enzyme activity and DNA damage after exercise, and the protective effect of garlic against exercise-induced oxidative stress. Male Sprague-Dawley rats(4 weeks old) were randomly divided into three groups of 6 rats each; control group(Con) without garlic and exercise, Ex group with exercise alone, and Ex-G group with 2% garlic and exercise. For 4 weeks, rats were given diets containing 15% corn oil and 1% cholesterol with or without garlic. The swimming was selected as a model for exercise performance. Rats swam for 40 min a day, for 5 days a week. Group Ex and Ex-G showed significant lowering in body weight gain and fat accumulation compared to control. No significant changes were observed in levels of plasma cholesterol and triglyceride among three groups, demonstrating that exercise and garlic had no effects on changes of blood lipid. This finding of blood lipid seems to be due to higher plant sterol content in corn oil. The DNA tail moment of lymphocytes showed greater tendency in Ex and Ex-G than in control, but garlic supplements failed to suppress DNA damages. Compared to control, Ex had higher plasma TBARS which was lowered to the control's level in Ex-G with 2% garlic supplementation(p<0.05). Ex-G led to a higher hepatic superoxide dismutase(SOD) activity than control and Ex(p<0.05). Activity of hepatic catalase was also increased in Ex-G, while in Ex it was significantly low(p<0.05). It seemed that TBARS levels were related to the activities of SOD and catalase, and that garlic contributed to increasing the enzyme activities and led to decrease of TBARS. These results demonstrate that lipid peroxidation and DNA damage occur as a consequences of oxidative stress after exercise, and that antioxidant defense against oxidative stress could be enhanced by garlic supplementation through the induction of antioxidant enzymes. However, further investigations should be done on the garlic effect on DNA damage.

Keywords

References

  1. Amagase H, Petesch BL, Matsuura H, Kasuga S. Intake of Garlic and its bioactive compounds. J Nutr 2001; 131: 955S-962S
  2. Block E. The chemistry of garlic and onion. Sci Am 1985; 252: 114-119
  3. Neil H, Sigali C. Garlic, its cardioprotective properties. Curr Top Lipodol 1994; 5: 6-10 https://doi.org/10.1097/00041433-199402000-00002
  4. Ali M, Thomson M. Consumption of garlic clove a day could be beneficial in preventing thrombosis. Prostaglandins Leukotr EssentI Fatty Acids 1995; 53: 211-212 https://doi.org/10.1016/0952-3278(95)90118-3
  5. Borek C. Free radical processes in multistage carcinogenesis. Free Radic Res Commun 1991; 12: 745-750
  6. Borek C. Antioxidants and cancer. Sci Med 1997; 4: 51-62
  7. Gutteridge JMC. Free radicals in disease precesses: a compilation of cause and consequence. Free Radic Res Commun 1993; 19: 141-158 https://doi.org/10.3109/10715769309111598
  8. Borek C. Antioxidant health effects of aged garlic extract. J Nutr 2001; 131: 1010S-1015S
  9. Stavric B. Role of chemopreventers in human diet. Clinical Biochemistry 1994; 27: 319-332 https://doi.org/10.1016/0009-9120(94)00039-5
  10. Wei Z, Lau BHS. Garlic inhibits free radical generation and augments antioxidant enzyme activity in vascular endothelial cells. Nutr Res 1998; 18: 61-70 https://doi.org/10.1016/S0271-5317(97)00200-5
  11. Ide N, Lau BHS. Garlic compounds protect vascular endothelial cells from oxidized low density lipoprotein-induced injury. J Pharm Pharmacol 1997; 49: 908-911 https://doi.org/10.1111/j.2042-7158.1997.tb06134.x
  12. Friedwald WT, Levy RI, Fredrickson DS. Estimation of concentration of low density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clin Chem 1972; 18: 499-502
  13. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95: 351-358 https://doi.org/10.1016/0003-2697(79)90738-3
  14. Fidanza F. Erythrocyte superoxide dismutase. In: Nutritional status assessment, p.398-401, Chapman & Hall, 1991
  15. Aebi H. Catalase. In: Bergmeyer HU, ed. Methods of enzymatic analysis, Chemie, Weinheim, FRG p.673-684, 1974
  16. Flohe L, Gunzler WA. Assays of glutathione peroxidase. In: Packer L, ed. Methods in enzymology vol 105, p.114-121, Academic press, 1984
  17. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantification of low levels of DNA damage in individual cells. Exp Cell Res 1988; 175: 184-191 https://doi.org/10.1016/0014-4827(88)90265-0
  18. Burneiko RCM, Diniz YS, Faine LA, Galhardi CM, Padovani CR, Novelli ELB, Cicogna AC. Impact of training program on lipid profile and cardic health. Biological Research 2004; 37: 53-59
  19. Bumeiko RCM, Diniz YS, Galhardi CM, Rodrigues HS, Ebaid GMX, Faine LA, Padovani CR, Cicogna AC, Novelli ELB. Interaction of hypercaloric diet and physical exercise on lipid profile, oxidative stress and antioxidant defenses. Food Chem Toxicology 2006; 44: 1167-1172 https://doi.org/10.1016/j.fct.2006.01.004
  20. Yeh YY, Liu L. Cholesterol-lowering effect of garlic extracts and organosulfur compounds: Human and animal studies. J Nutr 2001; 131 : 989S-993S
  21. Yeh YY, Yeh SM. Garlic reduces plasma lipids by inhibiting hepatic cholesterol and triacylglycerol synthesis. Lipids 1944; 29: 189-193 https://doi.org/10.1007/BF02536728
  22. Yoon GA. Effect of garlic supplement and exercise on plasma lipid and antioxidant enzyme system in rats. Korean J Nutr 2006; 39(1): 3-10
  23. Chetty KN, Calahan L,Harris KC, Dorsey W, Hill D, Chetty S, Jein SK. Garlic attenuates hypercholesterolemic risk factors in olive oil fed rats and high cholesterol fed rats. Pathophysiol 2003; 9: 127-132 https://doi.org/10.1016/S0928-4680(03)00002-6
  24. Liu L, Yeh YY. Inhibition of cholesterol biosynthesis by organosulfur compounds derived from garlic. Lipids 2000; 35: 197-203 https://doi.org/10.1007/BF02664770
  25. Takeuchi H, Kato T, Ikegarni H, Imai H, Takeuchi HI. REgulation of plasma and liver total cholesterol levels by dietary oleic acid in rats fed a high cholesterol diet. J Nutr Sci Vitaminol 1999; 45: 63-77 https://doi.org/10.3177/jnsv.45.63
  26. Lin EC, Fernandez ML, McNamara DZ. Dietary fat type and cholesterol quantity interact to affect cholesterol metabolism in guinea pig. J Nutr 1992; 122: 2019-2022 https://doi.org/10.1093/jn/122.10.2019
  27. Astley SB. Dietary antioxidants-pasr, present and future? Trends in Food Science and Technology 2003; 14: 93-98 https://doi.org/10.1016/S0924-2244(02)00281-9
  28. Jenkins RR. Free radical chemistry: relationship to exercise. Sports Med 1988; 5: 156-170 https://doi.org/10.2165/00007256-198805030-00003
  29. Zebra E, Komorowski TE. Free radical injury to skeletal muscle of young adult and old mice. Am J Physiol 1990; 258: S429-S435
  30. JI LL, Dillon D, Wu E. Alteration of antioxidant enzymes with aging in rat skeletal muscle and liver. Am J Physiol 1990; 258: R918-R923
  31. Buczynski A Kedziora J, Tkaczewski W, Wachowicz B. Effect of submaximal physical exercise on antioxidative protection of human blood platelets. In J Sports Med 1991; 12: 52-54 https://doi.org/10.1055/s-2007-1024655
  32. Laughglin MH, Simpson T, Sexton WL, Brown OR, Smith JK, Korthuis RJ. Skeletal muscle oxidative capacity, antioxidant enzymes and exercise training. J Appl Physiol 1990; 68: 2337-2343 https://doi.org/10.1152/jappl.1990.68.6.2337
  33. Shin MS, Kim WS, Kim YS, Kim SS. Exercise and oxidative stress. Korean J Exercise Nutr 1998; 2: 1-23
  34. Criswell D, Powers S, Dodd S. High intensity training-induced in skeletal muscle antioxidant enzyme activity. Med Sci sports exercise 1993; 25: 1135-1140
  35. Yamasaki Y, Li L, Lau BHS. Garlic compounds protect vascular endothelial cells from hydrogen peroxide-induced oxidant injury. Phytother Res 1994; 8: 408-412 https://doi.org/10.1002/ptr.2650080706
  36. Helen A, Rajasree CR, Krishnakumar K, Augusti KT, Vijayammal PL. Antioxidant role of oils isolated from garlic (Allium sativum Linn) and onion (Allium cepa Linn) on nicotine-induced lipid peroxidation. Vit Hum Toxicol 1999; 41: 316-319
  37. Hatman A, NieB AM, Grunert-Fuchs M, Poch B, Speit G. Vitamin E prevents exercise-induces DNA damage. Mutation Res 1995; 346: 195-202 https://doi.org/10.1016/0165-7992(95)90035-7
  38. Hartman A, Plappert U, Raddatz K. Grunert-Fuchs M, G Speit. Does physical activity induce DNA damage? Mutagenesis 1994; 9: 269-272 https://doi.org/10.1093/mutage/9.3.269