Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Development of the Eggs and Pre-Leptocephalus Larvae by Natural Spawning of Artificially-Matured Japanese Eel, Anguilla japonica

인위적 성숙 유도된 뱀장어 Anguilla japonica의 자연산란에 의한 난발생과 Pre-leptocephalus 자어의 발달

  • 김대중 (국립수산과학원 양식연구본부 양식관리팀) ;
  • 강언종 (남부내수면연구소) ;
  • 배준영 (국립수산과학원 양식연구본부 양식관리팀) ;
  • 박민우 (국립수산과학원 양식연구본부 양식관리팀) ;
  • 김응오 (남부내수면연구소)
  • Published : 2007.08.31
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Abstract

Embryonic and pre-leptocephalic larvae development of the eel, Anguilla japonica, are described following natural fertilization in the indoor tank of $23^{\circ}C$ water temperature. Following a routine hormone treatment technique for the brood stock, female eels were artificially matured by weekly intramuscular injections of salmon pituitary extracts (SPE) at a dosage of 20 mg/kg body weight (BW) for a total of 10-14 doses to induce ovarian maturation, while male eels received weekly intramuscular injections of human chorionic gonadotropin (HCG) at a dosage of 1 IU/g BW for a total of 6-10 doses to induce testicular maturation in a separate aquarium and induced natural spawning. Fertilized eggs of about 1.0 mm in diameter were pelagic and showed a typical discoidal cleavage. Hatching occurs 38 hrs after fertilization at a water temperature of $23^{\circ}C$. The newly hatched larvae measured about 3.0 mm in total length and the number of myomeres averages 42. Their mouths and anuses were opened at 4.5 days and the yolk sacs of the pre-leptocephalic larvae were almost absorbed at 6.5 days after hatching. Pre-leptocephalic larvae survive for 14.5 days. At this time they are $5.87{\pm}0.25mm$ in total length and have about 98 myomeres. However, morphological characterization of embryonic and pre-leptocephalic larvae were not different between natural fertilization and artificial fertilization by the dry method.

극동산 뱀장어의 자연 수정란을 이용하여 $23{\pm}0.5^{\circ}C$의 여과 해수가 공급되는 실내수조에서 난발생과 pre-leptocephalus 자어발달을 관찰하였다. 친어 관리에 있어서 암컷은 연어 뇌하수체 추출물(20 mg/fish)과 수컷은 HCG (1 IU/g BW)를 매주 복강 주사하여 인위적인 성성숙 및 자연산란을 유도하였다. 수정란은 직경 1.0 mm 정도의 분리 부성란으로, 전형적인 반상분할(discoidal cleavage)을 통해 분할이 이루어졌다. 부화는 $23^{\circ}C$의 여과 해수에서 수정 후 38시간부터 개시되었다. 부화 직후의 자어는 전장 약 3.0 mm, 근절수는 평균 42개였다. 부화자어의 입과 항문은 부화 후 4.5일째에 열렸고, 난황은 부화 후 6.5 일째에 거의 흡수되었다. 자어는 14.5일 동안 생존하였고, 이때 전장 $5.87{\pm}0.25mm$, 근절은 98개였다. 그러나 수정란과 prelaptocephalus 자어발생의 형태학적 특징은 자연수정과 건식법에 의한 인공수정과 큰 차이점은 없었다.

Keywords

References

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