YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Inhibitors of Nitric Oxide Syntheasis from Phellinus pini in Murine Macrophages

낙엽송층버섯의 Nitric Oxide 생성저해 물질

  • 장현진 (숙명여자대학교 약학대학) ;
  • 김안근 (숙명여자대학교 약학대학) ;
  • 표명윤 (숙명여자대학교 약학대학) ;
  • 양기숙 (숙명여자대학교 약학대학)
  • Published : 2007.12.31
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Abstract

The anti-inflammatory activity of fruit body of Phellinus pini was investigated by activity-guided fractionation. From the screening of each fraction for the inhibitory activity of NO production in lipopolysaccaride (LPS) activated RAW 264.7 cells, methanol extract and its hexane soluble fraction of Phellinus pini exhibited inhibition of NO production compared with LPS control without toxicity. The hexane soluble fraction showed dose-dependent inhibition of NO production. The active hexane fraction was repeatedly chromatographed over silica gel, ergosta-7,24(28)-dien-3-ol(1) and ergosterol peroxide (2) were isolated and identified. Ergosterol derivatives were inhibited NOS activation, $IC_{50}$ of them were $18.9{\pm}3.9{\mu}M$ (1) and $20.4{\pm}4.5{\mu}M$ (2).

Keywords

References

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