Inactivation Effect of Cryptosporidium by Ozone and UV

Ozone과 UV를 이용한 Cryptosporidium의 불활성화 효과

  • Kim, Yun-Hee (Department of Environmental Engineering, Yeungnam University) ;
  • Lee, Chul-Hee (Department of Environmental Engineering, Yeungnam University) ;
  • Lee, Shun-Hwa (Department of Environmental Engineering, Yeungnam University)
  • Published : 2007.01.31

Abstract

The objective of this study was to investigate the inactivation characteristics of Cryptosporidium oocysts by ozone and UV and to suggest the better, disinfection method. The inactivation CT value of 1 log(90%) and 2 log(99%) in of one disinfection, which is an index of disinfection for inactivation effect by ozone, were respectively 5.77 $mg{\cdot}min/L$ and 21.30 $mg{\cdot}min/L$. The inactivation in UV disinfection was not affected by pHs(5, 7 and 9), low turbidity(5 and below NTU) and UV intensity(0.2 and 0.6 $mWs/cm^2$) but obviously decreased at high turbidity over 20 NTU. Therefore UV disinfection capacity can be obtained when a good turbidity removal in drinking water treatment process is achieved. And if oocysts is exposed by high UV over 0.6 mWs/cm2 during enough time, the better inactivation effect will be obtained.

본 연구에서는 오존 및 UV 소독을 통해 Cryptosporidium 불활성화 특성을 파악하고 소독의 방향성을 제시하고자 하였다. ozone을 이용한 Cryptosporidium 불활성화 효과는 소독지표의 하나인 CT값$(mg{\cdot}min/L)$으로 평가할 수 있으며, 1 log(90%) 불활성화 CT값은 5.77 $mg{\cdot}min/L$이고 2 log(99%) 불화성화 CT값은 21.30 $mg{\cdot}min/L$인 것으로 나타났다. UV 소독을 통한 Cryptosporidium oocysts 불활성화 효과의 경우 pH(5, 7, 9)와 저탁도(5 NTU 이하) 그리고 UV 강도(0.2, 0.6 $mWs/cm^2$)에 따른 효과는 뚜렷하게 나타나지 않았다. 그러나 20 NTU 이상의 고탁도인 경우에는 불활성 효과가 뚜렷하게 감소하였다. 따라서 정수처리공정에서 UV 소독을 실시할 경우 탁도를 충분히 낮춰야만 만족할 만한 소독 능을 얻을 수 있으며 높은 UV 강도에서 충분한 시간 동안 UV가 조사된다면 더 높은 Cryptosporidium oocysts의 불활성화 효과를 얻을 수 있을 것으로 사료된다.

Keywords

References

  1. Mac-Kenzi, W. R., 'A massive outbreak in Milwaukee of Cryptosporidium infection transmitted through the public water supply,' The New England Journal of Medicine, 333(3), 161-167(1994)
  2. Betancourt, W. Q., Rose, J. B., 'Drinking water treatment processes for removal of Cryptosporidium and Giardia,' Veterinary Parasitology, 26, 219-234(2004)
  3. Corona-Vaszuez, B., Samuelson, A., Rennecker, J. L., Marinas, B. J., 'Inactivation of Cryptosporidium parvum oocysts with ozone and free chlorine,' Water Res., 36, 4053-4063(2002) https://doi.org/10.1016/S0043-1354(02)00092-1
  4. Cho, M., Kim, R. H., Cho, S. H., Yoon, J. Y, 'Investigation of ozone reaction in river waters causing instantaneous ozone demand,' Ozone Science & Engineering, 25, 251-259(2003) https://doi.org/10.1080/01919510390481577
  5. USEPA, 'Alternative disinfectants and oxidants guidance manual,' EPA 815-R-99-0(1999)
  6. Rochelle, P. A., Upton, S. J., Montelone, B. A., and Woods, K., 'The response of Cryptosporidium parvum to UV light,' Trends in Parasitology, TREPAR 286(2004)
  7. 류호돈, '21세기 선진국형 음용수, 우리의 건강을 위협하 는 수인성 미생물 Cryptosporidum', 한국과학기술정보 연구원, 최종보고서(2005)
  8. Craik, S. A., Finch, G. R, Bolton, J. R., and Belosevic, M., 'Inactivation of Giardia muris cysts using medium-pressure ultraviolet radiation in filtered drinking water,' Water Res., 34(18), 317-326(2000)
  9. Craik, S. A., Weldon, D., Finch, G. R., Bolton, J. R., Belosevic, M., 'Inactivation of Cryptosporidium parvum using medium-and low-pressure ultraviolet radiation,' Water Res., 35(6), 1387-1398(2001) https://doi.org/10.1016/S0043-1354(00)00399-7
  10. Huffman, D. E., Slifko, T. R., Salisbury, K., and Rose, J. B., 'Inactivaton of bacteria, Virus and Cryptosporidium by a point-of-use device using pulsed broad spectrum white light,' Water Res., 34(9), 2491-2498(2000) https://doi.org/10.1016/S0043-1354(00)00014-2
  11. Qian, S. S., Donnelly, M., Schmelling, D. C, Messner, M., Linden, K. G, Cotton, C, 'Ultraviolet light inaciva-tion of protozoa in drinking water: a Bayesian metaanalysis,' Water Res., 38, 317-326(2004) https://doi.org/10.1016/j.watres.2003.10.007
  12. 麻布大學環境保健學部健康環境科學科 水環境學硏究室, クリプトスポリジウム試驗法, Ver.3.2(1999)
  13. Campbell, A. T., Robertson, L. J., and Smith, H. V., 'Viability of Cryptosporidium parvum Oocysts : Correlation of In Vitro Excystation with Inclusion or Exclustion of Fluorogenic Vital Dyes,' Applied and Environmental Microbiology, 58(11), 3488-3493(1992)
  14. Black, E. K., Finch, G. R., Taghi-Kilanni, R., Belosevic, M., 'Comparison of assays for Cryptosporidium parvum oocysts viability after chemical disinfection,' FEMS Microbiology Letters, 135, 187-189(1996) https://doi.org/10.1111/j.1574-6968.1996.tb07987.x
  15. Hirata, T., Chikuma, D., Shimura, A., Hashimoto, A., Motoyama, N., Takahashi, K., Moniwa, T., Kaneko, M., Saito, S., and Maede, S., 'Effects of ozonation and chlo-rination on viability and infectivity of Cryptosporidium parvum oocysts,' Water Sci. Technol., 41(7), 39-46 (2000)
  16. 田中義行, 高和橋孝, 本山信行, 'クリプトスポリジウム對策としてのオジン消毒技術', 富士時報, 71(6), 336-341(1998)
  17. 本山信行, 小澤克行, 平田强, 星川 寬, 茂庭竹生, 金子光美, 'オジンによる Cryptosporidium parvum オ-シスト不活化能に關する實驗的檢討', 水道協會雜誌, 69(1)(1998)
  18. Hirata, T., Shimura, A., Morita, S., Suzuki, M., Motoyama, N., Hoshikawa, H., Moniwa, T., and Kaneko, M., 'The effect of temperature on the efficacy of ozonation for inacivating Cryptosporidium parvum oocysts,' Water Sci., Technol., 43(12), 163-166(2001)
  19. Vasquez, B. C, Samuelson, A., Rennecker, J. L., Marinas, B. J., 'Inactivation of Cryptosporidium parvum oocysts with ozone and free chlorine,' Water Res., 36, 4053-4063(2002) https://doi.org/10.1016/S0043-1354(02)00092-1
  20. 김윤희,'낙동강 중류수계에서 Cryptosporidium 분포와 정수처리공정에서의 제거 효율', 영남대학교 대학원 박사학위논문(2005)
  21. Hancock, C. M., Ward, J. V., Hancock, K. W., and Klonicki, P. T., 'Assessing plant performance using MPA : Knowledge of microbiotic particulate composition provides valuable information for assessing treatment plant performance and enhancing plant operation,' J. AWWA, 88(12), 24-34(1996) https://doi.org/10.1002/j.1551-8833.1996.tb06659.x
  22. 염철민, 조순행, 정해웅, 정현미, 윤제용, '하수소독을 위한 자외선의 Bacillus subtilis spore 불활성화 특성', 상하수도학회지, 15(4), 293-301(2001)
  23. Mano, S., Eugene, W. R., Benito, J. M., 'A Bayesian method of estimating kinetic parameters for the inac-tivation of Cryptosporidium parvum oocysts with chlorine dioxide and ozone,' Water Res., 37, 4533-4543(2003) https://doi.org/10.1016/S0043-1354(03)00412-3
  24. Biswas, K., Craik, S., Smith, D. W., Belosevic, M., 'Synergistic inactivation of Cryptosporidium parvum using ozone followed by free chlorine in natural water,' Water Res., 37, 4737-4747(2003) https://doi.org/10.1016/S0043-1354(03)00435-4