Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Isolation and Characterization of an Alkaline Protease Produced by Bacillus subtilis JK-1

알칼리성 Pretease를 생산하는 Bacillus subtilis JK-1의 분리 동정 및 효소 특성

  • Kim, Ji-Yeon (Graduate School of Molecular & Biomedical Technology, College of General Education, Inje University)
  • Published : 2007.12.30
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Abstract

A bacterium producing the alkaline pretense was isolated from Chungkookjoug, and was identified as Bacillus subtilis JK-1 based on morphological, physiological and biochemical characteristics, as well as phylogenetic analysis using 16S rRNA gene sequence. The optimum pH and temperature of the pretense activity were pH 9.0 and $55^{\circ}C$, respectively. This enzyme was stable at the temperatures $40{\sim}80^{\circ}C$. The maximum alkaline pretense production was obtained when 1.0% (w/v) xylose, 1.0% (w/v) yeast extract and 0.3% (w/v) $CuSO_4$ were used as carbon source, nitrogen source and mineral source. Under the optimal condition, growth of the isolate was reached at stationary phase after 12 hr followed by incubation, the alkaline pretense production reached a maximum level with $16{\sim}20$ hr cultivation.

청국장으로부터 알칼리성 pretense 생성이 우수한 균주를 분리한 후 형태적, 생리.생화학적 특성 및 16S rRNA 유전자 염기서열을 통한 계통분석을 이용하여 동정을 실시한 결과 Bacillus subtilis JK-1으로 판명되었다. B. subtilis JK-1이 생산하는 pretense의 최적 활성 pH와 온도는 각각 9.0과 $55^{\circ}C$이었으며, $40{\sim}80^{\circ}C$의 sh도에서 안정하였다. 본 균주는 배지 중에 탄소원과 질소원, 무기염으로 1.0% (w/v) xylose와 1.0% (w/v) yeast extract, 0.3% (w/v) $CuSO_4$를 사통하였을 경우 최대의 알칼리성 protease 생산성을 나타내었다. B. subtilis JK-1의 생육은 배양 후 12시간만에 최대 성장을 나타냈으며, 효소 활성은 8시간부터 급격히 증가하여 $16{\sim}20$시간에 최대 활성을 나타내었다.

Keywords

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