Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
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Development of Species-specific Molecular Marker as a Tool for Discrimination between Crucian Carp Gengorobuna (Carassius cuvieri) Introduced from Japan and Korean Native One (C. auratus)

국내 자연산 붕어와 일본에서 도입된 떡붕어를 구분하기 위한 종특이적 분자마커 개발

  • Song, Kyo-Hong (School of Biological Sciences, Seoul National University) ;
  • Jung, Jong-Woo (School of Biological Sciences, Seoul National University) ;
  • Koo, Hye-Young (Department of Biological Science, College of Natural Science and Engineering, Sangji University) ;
  • Kim, Won (School of Biological Sciences, Seoul National University)
  • 송교홍 (서울대학교 생명과학부) ;
  • 정종우 (서울대학교 생명과학부) ;
  • 구혜영 (상지대학교 생명과학과) ;
  • 김원 (서울대학교 생명과학부)
  • Published : 2007.03.30
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Abstract

The introduced exotic species has often caused severe problems to the native ecosystem. One of such species is the freshwater fish gengorobuna (Carassius cuvieri) introduced from Japan. The first step to assess harmful effects of this species on the Korean freshwater ecosystem is to discriminate it from the most similar native crucian carp (Carassius auratus). Because traditional morphological identification often gives unreliable results due to their highly similar phenotype, a new more efficient method is needed. For this purpose, molecular markers produced by the efficient one-step PCR method using three primers (DDF, DDR and DDR1) were developed and tested in the present study. This molecular marker will play an important role in monitoring fish community of Korean freshwater ecosystem.

외래 도입종은 국내 생태계에 빈번히 심각한 문제점들을 일으켜왔고, 이러한 종 중의 하나가 일본에서 도입된 담수어류인 떡붕어 (Carassius cuvieri)이다. 국내 담수생태계에서 떡붕어의 해로운 영향들을 평가하기 위해 우선적으로 선행되어야 할 연구는 형태적으로 가장 유사한 종(Corassius auratus)으로부터 이들을 구분해야 하는 것이다. 전통적인 형태적 동정은 이들 종의 매우 유사한 표현형으로 인해 신뢰할 수 없는 결과들을 자주 보여주기 때문에 더욱 효과적인 방법이 필요하다. 이를 위해 본 연구에서는 3종류의 프라이머(DDF, DDR 그리고 DDR1)를 이용한 효율적인 one-step PCR분자마커를 개발 적용하였다. 이 분자마커는 국내 담수 생태계의 어류 군집 모니터링에 중요한 역할을 할 것으로 기대된다.

Keywords

References

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