The Korea Journal of Herbology (대한본초학회지)
- Volume 22 Issue 4
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- Pages.253-260
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- 2007
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- 1229-1765(pISSN)
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- 2288-7199(eISSN)
Microarray-Based Gene Expression Profiling to Elucidate the Effectiveness of Woowhangchongshim-won on ICH Model in Rats
Microarray 분석법 활용을 통한 뇌출혈 흰쥐에서의 우황청심원 효능 평가
- Kim, Hyung-Woo (College of Oriental Medicine, Dongshin University) ;
- Cho, Su-Jin (College of Medicine, Korea University) ;
- Kim, Bu-Yeo (College of Oriental Medicine, Dongshin University) ;
- Jeong, Byeong-Han (College of Oriental Medicine, Dong-Eui University) ;
- Bong, Sung-Jeon (College of Oriental Medicine, Dong-Eui University) ;
- Kim, Yong-Seong (College of Oriental Medicine, Dongshin University) ;
- Lee, Jang-Sik (College of Oriental Medicine, Dongshin University) ;
- Kwon, Jeong-Nam (College of Oriental Medicine, Dong-Eui University) ;
- Kim, Young-Kyun (College of Oriental Medicine, Dong-Eui University) ;
- Cho, Su-In (College of Oriental Medicine, Dongshin University)
- 김형우 (동신대학교 한의과대학) ;
- 조수진 (고려대학교 의과대학) ;
- 김부여 (동신대학교 한의과대학) ;
- 정병한 (동의대학교 한의과대학) ;
- 봉승전 (동의대학교 한의과대학) ;
- 김용성 (동신대학교 한의과대학) ;
- 이장식 (동신대학교 한의과대학) ;
- 권정남 (동의대학교 한의과대학) ;
- 김영균 (동의대학교 한의과대학) ;
- 조수인 (동신대학교 한의과대학)
- Published : 2007.12.31
Abstract
Objectives : Intracerebral hemorrhage (ICH) is characterized by breakdown of blood vessels within the brain parenchyma. Fundamental therapeutic strategies for ICH, particularly those aimed at neuroprotection, have to be established. So in this experiment, the effects of Woowhangchongshim-won, a traditional prescription formula for treating Cerebral Apoplexy in Asian countries, were investigated. Methods : After intraperitoneal injection of chloralhydrate, rats were placed in a stereotaxic frame. ICH was induced by injection of 1 U collagenase type IV and drug was administered orally for 10 days. The molecular profile of cerebral hemorrhage in rat brain tissue was measured using micro array technique to identify up- or down- regulated genes in brain tissue. These genes induced by brain damage were mainly concerned with general metabolic process such as primary metabolic process, cellular metabolic process, macromolecule metabolic process, and biosynthetic process. Results : The number of genes increased in control and not-changed in experiment was 374, and decreased in control and not-changed in experiment was 527. We are concerned with genes that can be recovered by treatment with medicine, it is especially interesting to above types of genes. Conclusions : Upon medicine treatment to the rat having cerebral hemorrhage, expressions of some genes were restored to normal level. Further analysis using protein interaction database identified some key molecules that can be used for elucidation of therapeutical mechanism of medicine in future.