Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Swinging Effect of Salicylic Acid on the Accumulation of Polyhydroxyalkanoic Acid (PHA) in Pseudomonas aeruginosa BM114 Synthesizing Both MCL- and SCL-PHA

  • Rho, Jong-Kook (Division of Applied Life Sciences (BK21), Graduate School, Gyeongsang National University) ;
  • Choi, Mun-Hwan (Division of Applied Life Sciences (BK21), Graduate School, Gyeongsang National University) ;
  • Shim, Ji-Hoon (Division of Applied Life Sciences (BK21), Graduate School, Gyeongsang National University) ;
  • Lee, So-Young (Nano-Biomaterials Science Laboratory, Graduate School, Gyeongsang National University) ;
  • Woo, Myeong-Ji (Division of Applied Life Sciences (BK21), Graduate School, Gyeongsang National University) ;
  • Ko, Bong-Sung (Department of Chemistry, University of Ulsan) ;
  • Chi, Ki-Whan (Department of Chemistry, University of Ulsan) ;
  • Yoon, Sung-Chul (Division of Applied Life Sciences (BK21), Graduate School, Gyeongsang National University)
  • Published : 2007.12.31
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Abstract

A bacterium, Pseudomonas aeruginosa BM114, capable of accumulating a blend of medium-chain-length (MCL)- and short-chain-length (SCL)-polyhydroxyalkanoic acid (PHA), was isolated. Salicylic acid (SA), without being metabolized, was found to specifically inhibit only the accumulation of MCL-PHA without affecting cell growth. An addition of 20 mM SA selectively inhibited the accumulation of MCL-PHA in decanoate-grown cells by 83% of the control content in one-step cultivation, where overall PHA accumulation was inhibited by only ${\sim}11%$. Typically, the molar monomer-unit ratio of the PHA for 25 mM decanoate-grown cells changed from 46:4:25:25 (=[3-hydroxybutyrate]:[3-hydroxycaproate]: [3-hydroxyoctanoate]:[3-hydroxydecanoate]) at 0 mM SA (dry cell wt, 1.97 g/l; PHA content, 48.6 wt%) to 91:1:4:4 at 20 mM SA (dry cell wt, 1.85 g/l; PHA content, 43.2 wt%). Thus, the stimulation of SCL-PHA accumulation was observed. Growth of P. aeruginosa BM114 on undecanoic acid also produced a PHA blend composed of 47.4% P(3HB-co-3-hydroxyvalerate) and 52.6% P(3-hydroxyheptanoate-co-3-hydroxynonanoate-co-3-hydroxyundecanoate). Similar to the case of even-carboxylic acids, SA inhibited the accumulation of only MCL-PHA, but stimulated the accumulation of SCL-PHA. For all medium-chain fatty acids tested, SA induced a stimulation of SCL-PHA accumulation in the BM114 strain. SA could thus be used to suppress only the formation of MCL-PHA in Pseudomonas spp. accumulating a blend of SCL-PHA and MCL-PHA.

Keywords

References

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