Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Identification of Fungal Strains Producing Gibberellins from the Root of plants

식물뿌리내부에 존재하는 지베렐린 생산균 분리와 동정

  • Rim, Soon-Ok (Department of Microbiology, Kyungpook National University) ;
  • Lee, Jin-Hyung (Department of Microbiology, Kyungpook National University) ;
  • Khan, Sumera Afzal (Department of Microbiology, Kyungpook National University) ;
  • Lee, In-Jung (Department of Agromony, Kyungpook National University) ;
  • Rhee, In-Koo (Department of Agricultural Chemistry, Kyungpook National University) ;
  • Lee, Kyung-Soo (Division of Food, Beverage and Culinary Art, Yeungnam College of Science and Technology) ;
  • Kim, Jong-Guk (Department of Microbiology, Kyungpook National University)
  • Published : 2007.12.28
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Abstract

249 fungal strains were isolated from the roots of 26 plants, and the production of GAs was spectrophotometric ally examined. As a result 76 fungal strains were shown to produce GAs. Bioassay of culture broth from seventy six fungal strains producing GAs was carried out with waito-c rice, that is dwarf rice. The seventy six fungi with GAs-producing activity were incubated for seven days in 40 mL of Czapek's liquid medium at $30^{\circ}C$ and 180 rpm, and the culture broth of fungi were treated on the 2-leaf rice sprout. Fifteen of these showed plant growth promoting activity and the amount of each GAs in the medium was measured by Gas chromatographymass spectrometer (GC-MS). Nine of these fungi were also identified by genetic analysis of the nucleotide sequences in the internal transcribed spacer region of the ribosomal DNA.

Keywords

References

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