Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Association of Sequence Variations in DGAT 1 Gene with Economic Traits in Hanwoo (Korea Cattle)

  • Kong, H.S. (Genomic Informatics Center, Hankyong National University) ;
  • Oh, J.D. (Genomic Informatics Center, Hankyong National University) ;
  • Lee, J.H. (Genomic Informatics Center, Hankyong National University) ;
  • Yoon, D.H. (National Livestock Research Institute, RDA) ;
  • Choi, Y.H. (National Livestock Research Institute, RDA) ;
  • Cho, B.W. (Pusan National University) ;
  • Lee, H.K. (Genomic Informatics Center, Hankyong National University) ;
  • Jeon, G.J. (Genomic Informatics Center, Hankyong National University)
  • Received : 2006.07.05
  • Accepted : 2007.01.04
  • Published : 2007.06.01
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Abstract

The effects of diacylglycerol O-acyltransferase (DGAT1) candidate gene polymorphism on the economic traits of Hanwoo were studied. Through sequencing analysis, two polymorphism sites at K232A and T11993C were established and were analyzed by PCR-RFLP. The PCR-RFLP analysis for K232A showed that the frequencies of alleles K and A were 0.75 and 0.25, respectively, and the frequencies of genotypes for K/K, K/A and A/A were estimated as 0.509, 0.491 and 0, respectively. In the PCR-RFLP analysis for T11993C, we found allele frequencies of 0.773 and 0.227 for T and A, respectively, and 0.546, 0.454 and 0 for the T/T, T/C and C/C genotype frequencies, respectively. No significant effects on economic traits in Hanwoo were found in the separate analysis of K232A and T11993C polymorphisms, but the interaction between K232A and T11993C showed a significant effect (p<0.005) on marbling score. The DGAT1 candidate gene was found to have a significant effect not only on milk yield and component traits but also on the metabolism of intramuscular fat.

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References

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