Anatomy and Cell Biology

Korean Association of Anatomists (대한해부학회)
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Changes of BDNF (Brain Derived Neurotrophic Factor) Expression Associated with Urodynamic Changes in Rat Spinal Cord Injury Animal Model

흰쥐 척수손상(spinal cord injury) 동물모델에서 뇨역동 (urodynamics)의 변화와 연관된 BDNF의 발현변화

Lee, Young-Il;Hyun, Jung-Keun
이영일;현정근

  • Published : 20070600
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Abstract

Spinal cord injury results in dramatic changes in the neurochemistry of peripheral and central micturition reflex pathways. We studied an animal model of spinal cord contusion injury using Sprague Dawley rats. Recoveries of motor and bladder functions were recorded, along with the changes in brain-derived neurotrophic factor (BDNF) in regions rostral and caudal to the injury site. Results are as follows: 1. Motor functions examined by the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale were fully recovered 28 d after spinal cord injury. 2. Bladder functions, monitored urodynamically, changed from flaccid paralysis at 4 d after spinal cord injury to spastic paralysis at 14, 18, and 28 d. 3. BDNF immunoreactive neurons and glial cells were found in both gray and white matters of the normal spinal cord, and the numbers decreased gradually after spinal cord injury 4. BDNF enzyme linked immunosorbent assay (ELISA) results were almost the same as for immunohistochemistry, but the intensity of decrease was more prominent in the caudal than in the rostral regions. Distinguishing between the beneficial or detrimental effects of neurotrophic factors in the context of micturition reflexes or regenerative responses will be a challenge, but is essential to understanding the effects of therapies directed at blocking the effects of neuroactive compounds or neurotrophic factors.

본 연구의 목적은 척수의 손상에 따른 방광의 기능적 변화와 척수에서의 신경화학적 변화, 그리고 운 동기능의 소실과 회복에 대해 시기별로 동시에 비교분석하는 데 있다. 먼저 실험동물의 척수에 가해지는 충격의 양을 표준화할 수 있는 MASCIS 충격기(Multicenter Animal Spinal Cord Injury Study impactor, Rutgers university of New Jersey, USA)를 사용하여 흰쥐의 척수에 타박손상을 가하였다. 손상 후에는 시기별로 1) 행동학적 변화 관찰, 2) 뇨역동학검사(urodynamic study)를 통한 방광의 기능변화 관찰, 3) BDNF의 발현변화를 모두 한 개체에 서 각각 시행하였으며, 결과는 다음과 같다. 1) BBB (Basso, Beattie, Bresnahan) 이동평가척도(locomotor rating scale)를 기준으로 척수손상 후에 나타난 운동기능의 상실과 회복을 측정한 결과, 손상 후 약 한 달 정도의 시간 을 거치면서 서서히 회복되는 양상을 보였다. 2) 뇨역동학검사를 통해 척수손상 후의 방광기능을 측정한 결과, 운동기능이 회복되는 시점에서도 여전히 강직마비(spastic paralysis)의 양상을 보여 자율신경계통은 회복되지 않은 것으로 나타났다. 3) 척수손상 후 BDNF의 발현은 손상 후의 시간에 따라 점차 감소하였으며, 손상부위의 머리쪽(rostral)보다 꼬리쪽(caudal) 부위에서의 감소가 더욱 현저하였다. 결론적으로 척수 손상 후의 자율신경기 능의 회복은 이와 관련된 신경화학적 변화와 더불어 운동기능의 회복보다 더디게 진행되는 것을 확인하였다. 척 수손상 후의 운동기능 변화와 방광기능의 변화, 그리고 척수에서의 신경화학적 변화에 대한 신뢰성 있는 비교분 석을 통해 얻은 본 연구의 결과는 척수손상 동물모델을 통한 병태생리적 기전과 치료법 연구를 활성화하는 계 기가 될 것이다.

Keywords

References

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