Anatomy and Cell Biology

Korean Association of Anatomists (대한해부학회)
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The Effect of Yacon (Smallanthus sonchifolius) Extract against Dibutyltin Dichloride-induced Pancreatitis

야콘 (Smallanthus sonchifolius) 추출물이 Dibutyltin Dichloride로 유발된 췌장염에 미치는 영향

Choi, Nan-Hee;Choi, Seong-Hun;Lim, Seong-Woo;Park, In-Sick
최난희;최성훈;임성우;박인식

  • Published : 20070900
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Abstract

Yacon is used in folk medicines as a medicinal tea for hypoglycemia. In a recent study described herein, anti-oxidative, -bacterial, -fungal activities and cell protective functions of leaf extract of Smallanthus sonchifolius have been reported. In this study, to evaluate the effect on pancreatitis by using yacon extract (YE), we examined the efficacy of YE on DBTC (8 mg/kg)-induced pancreatitis in rats. After the treatment of DBTC on day 21, highly increased inflammatory cells, necrosis of acinar cells and connective tissues were observed. However, the inflammation was strongly inhibited in YE as compared to control group (CON). As a result of immunoreactions against HSP27, HSP70 and MAC387, weak or no possibilities of normal group (NOR) were observed in pancreas, whereas in immunoreactions against HSP27 and HSP70, strong expression of CON was observed in acinar and duct cells on day 14. However, the expression of HSP27 was decreased in acinar cells for CON treatment on day 21 as compared to 14 days observation, whereas strong expression of HSP27 was observed in duct cells and blood vessels on day 21. As regards the immunoreactions against HSP70, weak or no possibilities of CON were observed in duct cells and blood vessels as well as in acinar cells on day 21. In CON, time-dependent increase of MAC387 was observed. However, the expressions of HSP27 and HSP70 were weakly observed in YE as compared to CON on day 14 with strong positive reaction in acinar cells on day 21. Immunoreactions against MAC387 were decreased in YE on day 14 than day 7, which increased on day 21 as compared to day 14. These results suggested that yacon extract has inhibitory and mitigatory effects against DBTC-induced pancreatitis.

야콘은 민간 요법에서 고혈당에 대한 차로 이용되고 있으며, 최근 연구에서, 야콘잎은 항산화, 항균, 항 진균 효과 및 세포 보호기능이 있음이 보고되고 있다. 본 연구에서는 야콘 추출물에 의한 췌장염의 효과를 확인 해 보기 위하여 DBTC (8 mg/kg)로 유발된 췌장염에서 그 효과를 실험하였다. 췌장염 유발군은 DBTC 투여 21 일째 염증관련 세포의 급증과 샘꽈리세포의 괴사가 심해지면서 주변의 결합조직이 매우 증가된 반면, 1% 야콘 추출물을 음용한 처리군에서는 유발군에 비해 염증 진행이 매우 억제되어 있었다. HSP27, HSP70, MAC387에 대한 면역반응에서 정상군은 모두 양성반응이 거의 나타나지 않았으나, 유발군의 HSP27과 HSP70에 대한 면역 반응에서는 췌장염 유발 14일째까지 샘꽈리세포와 도관세포에서 양성반응이 증가되었으며, 21일째 HSP27에 대 한 면역반응은 샘꽈리세포에서는 오히려 감소되고 도관과 혈관에서는 강한 양성반응을 나타낸 반면, HSP70의 면역반응은 샘꽈리세포와 도관 및 혈관에서도 거의 나타나지 않거나 매우 약한반응을 보였다. MAC387에 대한 면역반응에서 유발군은 시간이 경과되면서 면역반응을 나타내는 세포가 증가하였다. 반면, 야콘 추출물을 음용 한 군의 HSP27과 HSP70에 대한 면역반응은 DBTC투여 후 14일째까지 약한반응을 보이다가 21일째 강한 양 성반응을 나타내는 세포가 다소 증가되었으며, MAC387에 대한 반응은 7일째보다 14일째 오히려 감소한 경향 을 보였으며 21일째 다시 증가되었으나 유발군에 비해 매우 낮은 정도였다. 이러한 결과로, 1% 야콘 추출물은 췌장염에 대한 염증진행을 억제 및 완화시키는 효과가 있음을 확인하였다.

Keywords

References

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