Experimental and Molecular Medicine

Korean Society for Biochemistry and Molecular Biongy (생화학분자생물학회)
권호 표지

SIRT1 promotes DNA repair activity and deacetylation of Ku70

Jeong, Jae-Min;Juhn, Kyung-Mi;Lee, Han-Soo;Kim, Sang-Hoon;Min, Bon-Hong;Lee, Kyung-Mi;Cho, Myung-Haeng;Park, Gil-Hong;Lee, Kee-Ho

  • Published : 20070200
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Abstract

Human SIRT1 controls various physiological re-sponses including cell fate, stress, and aging, through deacetylation of its specific substrate protein. In processing DNA damage signaling, SIRT1 attenuates a cellular apoptotic response by deace-tylation of p53 tumor suppressor. The present study shows that, upon exposure to radiation, SIRT1 could enhance DNA repair capacity and deacetylation of repair protein Ku70. Ectopically over-expressed SIRT1 resulted in the increase of repair of DNA strand breakages produced by radiation. On the other hand, repression of endogenous SIRT1 expression by SIRT1 siRNA led to the decrease of this repair activity, indicating that SIRT1 can regulate DNA repair capacity of cells with DNA strand breaks. In addition, repair protein Ku70, leading to subsequent deace-tylation. The dominant-negative SIRT1, a cataly-tically inactive form, did not induce deacetylation of Ku70 protein as well as increase of DNA repair capacity. These observations suggest that SIRT1 modulates DNA repair activity, which could be regulated by the acetylation status of repair protein Ku70 following DNA damage.

Keywords

References

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