Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

Biological Activity of Varieties, Isolation and Purification of antioxidants components in sweetpotato

고구마 품종별 생리활성 및 항산화 성분 분리 정제

Lee, Joon-Seol;Ahn, Young-Sup;Chung, Mi-Nam;Kim, Hag-Sin
이준설;안영섭;정미남;김학신

  • Published : 2007.09.30
⟨ Previous Next ⟩

Abstract

　This study was conducted to investigate nitrite scavenging and Angiotensin Converting Enzyme (ACE) effects by antioxidant ingredient and the contents of phenolic compounds is isolated and purified from various sweetpotato cultivars. Various sweetpotato cultivars contained 4 phenol compounds including gallic acid, chlorogenic acid, gentisic acid and caffeic acid. The range of total phenol compound contents within cvs. Saengmi, Seonmi, Hongmi and Zami was at 23.0~48.8mg/100g and cv. Zami containing anthocyanins pigment whose total phenol compound content was at 48.8mg/100g was by far higher than the other cultivars. The assay of antioxidant activity using solvent fractionated extraction methods was investigated in various sweetpotato cultivars. Antioxidant activity of ethyl acetate (41.2~86.2%) was much higher than chloroform and butanol. Antioxidant activity from cvs. Sangmi, Seonmi, Hongmi, Shinyulmi, Zami and Sinchunmi was much higher than α-tocopherol well-known as natural antioxidant. Rancidity inhibitory effect about soybean oil of extracts fractionated by ethyl acetate from cvs. Saengmi, Seonmi, Hongmi and Zami was high considerably. Ethyl acetate layer among various solvent fractionated extraction had a good effect on nitrite scavenging rate but not had a effect on AEC inhibitory. Solvent of column chloromatography to isolate antioxidant contents from ethyl acetate layer was even better on 30% (MeOH) : 70% (Water) condition. There are 5 ingredients at HPLC chromatogram and antioxidant activity effect of individual ingredients was slightly better on Rt 11.9 and Rt 16.9 fraction.

Keywords

References

  1. Ahn YS, Jeong BC, Chung MN, Lee JS, Jeong KB, Oh YB, Jeong DH, Cho SY, Min KS, Lee JS, Kim ES, Kim GT. 2000. A new sweetpotato (Ipomoea batatas (L.) Lam.) cultivar 'Jinhongmi' for the edible and the starch. Korean J. Breed. 32(1 ):95-97
  2. Ahn YS, Jeong BC, Chung MN, Lee JS, Oh VB. 2002. A new purple- tlesh and high anthocyanin sweetpotato variety 'Sinjami'. Korean J. Breed. 34(4):379-380
  3. Brand W, Cuvelier ME, Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. Lebensm. Wiss. Technol. 28:25-30 https://doi.org/10.1016/S0023-6438(95)80008-5
  4. Cushman DW, Cheung HS, Sabo EF, Ondetti MA. 1997. Design of potent competitive inhibitors of angiotensin converting enzyme, carboxyalkanoyl and mercapoalkanoyl amino acids. Biochem. 16:54-84
  5. Gilbert S, Gary E, Mark D, John B, Mark R, Andrew G, James P, Frank L, Barbara Y, James H, Scott B, Samuel H. 1996. Effects of A combination of beta carotene and vitamin a on lung cancer and cardiovascular disease. England J. Medicine. 334(18):1150-1155 https://doi.org/10.1056/NEJM199605023341802
  6. Huang MT, Ferraro T. 1991. Phenolic compounds in food and cancer prevention. Chemical Society. Washington D.C. pp. 220-238
  7. Jeong BC, Ahn YS, Chung MN, Lee JS, Jeong KB, Oh YB, Jeong DH, Cho SY, Min KS, Lee JS, Kim ES, Kim GT. 2000. A new orange colored sweetpotato (Ipomoea batatas (L.) Lam.) cultivar 'Sinhwangmi' for the fresh and processing. Korean J. Breed. 32( I):93-94
  8. Kato H, Lee JE, Chuyen NV, Kim SB, Hayase F. 1987. Inhibition of oitrosamioe forrnmation by nondialyzable melanoidins. Agric. Biol. Chem. 51:1333-1338
  9. Lee JS, Park YK, Ahn YS, Kim HS, Jung MN, Jeong BC, Bang JK. 2007. Antioxidative and biological activites of extracts of swectpotato tips. Korean J. Crop. Sci. 52(2):228-238
  10. Lee JS, Ahn YS, Kim HS, Chung MN, Kim JJ, Kim JM, Jeong BC, Bang JK. 2006. A new sweetpotato cultivar for table use 'Yeonhwangmi'. Korean J. Breed. 38(2):149-150
  11. Peto R, Doll R, Buckley JD, Sporn MB. 1981. Can dietary beta-carotene materially reduce human cancer rates? Nature. 8:201-290 https://doi.org/10.1038/008201a0
  12. Rhodes D, Handa S, Bressan RA. 1986. Metabolic changes associated with adaptation of plant cells to water stress. Plant Physiol. 82:890-903 https://doi.org/10.1104/pp.82.4.890
  13. Shahidul Islam MD, Yoshimoto M, Yahara S, Okuno S, Ishiguro K, Yamakawa O. 2002. Identification and characterization of foliar polyphenolic composition in sweetpotato (Ipomoea batatas L.) genotypes. J. Agric. Food Chem. 50:3718-3722 https://doi.org/10.1021/jf020120l
  14. Sohn SM. 1996. Nitrate and overuse of organic fertilizer. Organic Agriculture Copenhagen. 11th IFOAM Sci. Confcrcnce. pp. 36
  15. Son S, Lewis BA. 2002. Free radical scavenging and antioxidative activity of caffeic amide and ester analogues (structure-activity relationship). J. Agric food. Chem. 50:468-472 https://doi.org/10.1021/jf010830b
  16. Woolfe JA. 1992. Sweet potato. Cambridage University Press. New York. USA
  17. 농촌진흥청. 2006. 고구마 재배. 표준영농교본 28. 농촌진흥청
  18. 연세대학교 근대한국학연구소. 2004. 영호 조엄과 고구마 전래. 연세대학교
  19. 정병춘, 안영섭, 2004. 고구마의 한국 전래와 발전 과정, 100주년 기념 세미나 목포시험장 농업연구 성과 및 발전방향. 농촌진흥청 작물과학원목포시험장. pp. 81-113