Laboraroty Animal Research

Korean Association for Laboratory Animal Science (한국실험동물학회)
권호 표지

Effects of Opuntia humifusa Extract on DNCB-Induced Allergic Contact Dermatitis in BALB/c Mice

Kim, Kil-Soo;Ahn, Mi-Jee;Kim, Gi-Sang;Park, Seung-Chun;Rhee, Man-Hee;In, Jun-Gyo;Kim, Bok-Hyun;Nah, Young-Lae;Kim, Hyun-Ho;Han, Seung-Ho

  • Published : 20070600
⟨ Previous Next ⟩

Abstract

This study was performed to assess the effects of Opuntia humifusa (OH) extract using a mouse model of allergic contact dermatitis. Allergic contact dermatitis (ACD) was induced on the ears of BALB/c mice caused by sensitized and repeated application of 2,4-dinitro-1-chlorobenzene (DNCB). Topical treatment of 10% OH solution was applied once a day for 3 days and ear swelling was measured daily. After 3 days of treatment the mice were sacrificed and ear samples were collected for cytokine determination and histological examination. The repeated application of DNCB induced a relatively steep increment of ear thickness. Significant reductions of swelling, erythema and histopathological inflammation in ears were observed after 3 days of treatment with 10% OH. OH significantly suppressed the levels of interleukin-1β (IL-1β), IL-2 and Interferon-ϒ (IFN-ϒ) in the ears and the levels of IgE in serum after DNCB rechallenge. This study demonstrates that OH inhibits DNCB-induced ACD in mice, and suppresses the levels of inflammatory cytokines, such as IL-1β, IL-2 and IFN-ϒ in the ear. Based on these findings, OH may have antiinflammatory and alleviatory effects in the ACD.

Keywords

References

  1. Abma E.M., Blanken R. and DeHeide L.J. (2002) Cushing syndrome caused by topical steroid therapy for psoriasis. Neth. J. Med. 60, 148-150
  2. Akhavan A. and Cohen S.R. (2003) The relationship between atopic dermatitis and contact dermatitis. Clin. Dermatol. 21, 158-162 https://doi.org/10.1016/S0738-081X(02)00372-3
  3. Brown, T. (2004) Strategies for prevention: occupational contact dermatitis. Occup. Med. (Lond.) 54, 450-457 https://doi.org/10.1093/occmed/kqh097
  4. Bruch-Gerharz D., Fehsel K., Suschek C., Michel G., Ruzicka T. and Kolba-Bachofen V. (1996) A proinflammatory activity of interleukin 8 in human skin: expression of the inducible nitric oxide synthase in psoriatic lesions and cultured keratinocytes. J. Exp. Med. 184, 2007-2012 https://doi.org/10.1084/jem.184.5.2007
  5. Bruch-Gerharz D., Ruzicka T. and Kolb-Bachofen V. (1998) Nitric Oxide in Human Skin: Current Status and Future Prospects. J. Invest. Dermatol. 110, 1-7 https://doi.org/10.1046/j.1523-1747.1998.00084.x
  6. Butera, D., Tesoriere, L., Di Gaudio, F., Bongiorno, A., Allegra, M., Pintaudi, A.M., Kohen, R. and Livrea, M.A. (2002) Antioxidant activities of sicilian prickly pear (Opuntia ficus indica) fruit extracts and reducing properties of its betalains: betanin and indicaxanthin. J. Agric. Food Chem. 50, 6895-6901 https://doi.org/10.1021/jf025696p
  7. Bwititi, P., Musabayane, C.T. and Nhachi, C.F. (2000) Effects of Opuntia megacantha on blood glucose and kidney function in streptozotocin diabetic rats. J. Ethnopharmacol. 69, 247-252 https://doi.org/10.1016/S0378-8741(99)00123-3
  8. Charman C., Morris A.D. and Williams H.C. (2000) Topical corticosteroid phobia in patients with atopic dermatitis. Br. J. Dermatol. 142, 931-936 https://doi.org/10.1046/j.1365-2133.2000.03473.x
  9. de La Barrera, E. and Nobel, P.S. (2004) Carbon and water relations for developing fruits of Opuntia ficus-indica (L.) Miller, including effects of drought and gibberellic acid. J. Exp. Bot. 55, 719-729 https://doi.org/10.1093/jxb/erh084
  10. Dok-Go, H., Lee, K.H., Kim, H.J., Lee, E.H., Lee, J., Song, Y.S., Lee, Y.H., Jin, C., Lee, Y.S. and Cho, J. (2003) Neuroprotective effects of antioxidative flavonoids, quercetin, (+)-dihydroquercetin and quercetin 3-methyl ether, isolated from Opuntia ficus-indica var. saboten. Brain Res. 965, 130-136 https://doi.org/10.1016/S0006-8993(02)04150-1
  11. Enk A.H. and Katz S.I. (1992) Early molecular events in the induction phase of contact sensitivity. Proc. Natl. Acad. Sci. USA. 89(4), 1398-1402
  12. Enk A.H., Angeloni V.L., Udey M.C. and Katz S.I. (1993) An essential role for Langerhans cell-derived IL-1 beta in the initiation of primary immune responses in skin. J. Immunol. 150(9), 3698-3704
  13. Galati, E.M., Tripodo, M.M., Trovato, A., Miceli, N. and Monforte, M.T. (2002) Biological effect of Opuntia ficus indica (L.) Mill. (Cactaceae) waste matter. Note I: diuretic activity. J. Ethnopharmacol. 79, 17-21 https://doi.org/10.1016/S0378-8741(01)00337-3
  14. Fisher D.A. (1995) Adverse effects of topical corticosteroids use. West. J. Med. 162, 123-126
  15. Florian, C.S. and Reinhold C. (2005) Cactus stems (Opuntia spp.): A review on their chemistry, technology, and uses. Mol. Nutr. Food Res. 49, 175-194 https://doi.org/10.1002/mnfr.200400071
  16. Fujii Y., Takeuchi H., Tanaka K., Sakuma S., Ohkubo Y. and Mutoh S. (2002) Effects of FK506 (tacrolimus hydrate) on chronic oxazoloneinduced dermatitis in rats. Eur. J. Pharmacol. 456(1-3), 115-121 https://doi.org/10.1016/S0014-2999(02)02554-2
  17. Kalish R.S. (1996) Recent developments in the pathogenesis of allergic contact dermatitis. Arch. Dermatol. 127, 1558-1563 https://doi.org/10.1001/archderm.127.10.1558
  18. Kim, Y.H., Park, S.C., Son, W.Y., Rhee, M.H., Kim, T.W., Han, S.H., Kim, B.H. and Kim, K.S. (2005a) Hepatoprotective effects of Opuntia humifusa against carbon tetrachloride-induced acute liver injury in rats. Laboratory Animal Research. 21(3), 263-266
  19. Kim, Y.H. and Park, T.S. (2006) Effect of Scutellaria baicalensis water extract on antioxidative activity and epidermal thickness in DNCB-induced allergic contact dermatitis animal model. J. Korean Soc. Food Sci. Nutr. 35(5), 543-548 https://doi.org/10.3746/jkfn.2006.35.5.543
  20. Kim, Y.R., Lee, M.R., Kim, Y.H., Jang, B.J., Park, S.C., Han, S.H., Kim, B.H., Ryoo, Z.Y. and Kim. K.S. (2005b) Effect of Opuntia humifusa extract on indomethacin-induced gastric ulcer in Sprague-Dawley rat. Lab. Anim. Res. 21(4), 375-378
  21. Kondo S. and Sauder D.N. (1995) Epidermal cytokines in allergic contact dermatitis. J. Am. Acad. Dermatol. 33, 786-800 https://doi.org/10.1016/0190-9622(95)91817-5
  22. Lee, E.B., Hyun, J.E., Li, D.W. and Moon, Y.I. (2002) Effects of Opuntia ficus-indica var. Saboten Stem on Gastric Damages in Rats. Arch. Pharm. Res. 25(1), 67-70 https://doi.org/10.1007/BF02975264
  23. Letko E., Bhol K., Pinar V., Foster C.S. and Ahmed A.R. (1999) Tacrolimus (FK 506). Ann. All. Asthma Immunol. 83, 179-190 https://doi.org/10.1016/S1081-1206(10)62636-1
  24. Maguire H.C., Jr. (1995) Murine recombinant IL-12 increases the acquisition of allergic contact dermatitis in the mouse. Int. Arch. Allergy Immunol. 106, 166-168 https://doi.org/10.1159/000236839
  25. Park, E.H., Kahng, J.H. and Paek, E.A. (1998) Studies on the pharmacological action of cactus: identification of its antiinflammatory effect. Arch. Pharm. Res. 21, 30-34 https://doi.org/10.1007/BF03216749
  26. Park, E.H. and Chun, M.J. (2001) Wound healing activity of Opuntia ficus-indica. Fitoterapia 72, 165-167 https://doi.org/10.1016/S0367-326X(00)00265-3
  27. Piguet P.F., Garu G.E., Hauser C. and Vassalli P. (1991) Tumor Necrosis Factor is a critical mediator in hapten-induced irritant and contact hypersensitivity reactions. J. Exp. Med. 173, 673-679 https://doi.org/10.1084/jem.173.3.673
  28. Retamo S., Remitz A. and Kyllchen H. (2002) Topical corticosteroid immunomodulation in the treatment of atopic dermatitis. Am. J. Clin. Dermatol. 3, 381-388 https://doi.org/10.2165/00128071-200203060-00002
  29. Schwarz T. (1995) Interleukin-12 and its role in cutaneous sensitization. Res. Immunol. 146, 494-499 https://doi.org/10.1016/0923-2494(96)83022-7
  30. Shornick L.P., De Togni P., Mariathasan S., Goellner J., Strauss- Schoenberger J., Karr RW., Ferguson T.A. and Chaplin D.D. (1996) Mice deficient in IL-1beta manifest impaired contact hypersensitivity to trinitrochlorobenzone. J. Exp. Med. 183(4), 1427-1436 https://doi.org/10.1084/jem.183.4.1427
  31. Stevens R.L. and Austen K.F. (1989) Recent advances in the cellular and molecular biology of mast cells. Immunol. Today 10, 381-386 https://doi.org/10.1016/0167-5699(89)90272-7
  32. Wiese, J., McPherson, S., Odden, M.C. and Shlipak, M.G. (2004) Effect of Opuntia ficus indica on symptoms of the alcohol hangover. Arch. Intern. Med. 164, 1334-1340 https://doi.org/10.1001/archinte.164.12.1334