Journal of the Korean Orthopaedic Association (대한정형외과학회지)

Korean Orthopaedic Association (대한정형외과학회)
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Agonist for the Control of Apotosis through the Study of Cytokine Expression after Spinal Cord Injury in Rats

쥐의 척수손상 후 사이토카인 발현 연구를 통한 세포 사멸을 조절하는 길항제의 연구

Yang, Jun-Young;Riew, K. Daniel
양준영

  • Published : 20070200
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Abstract

Purpose : To analyze the cytokines that appear after a spinal cord injury in rats and to determine the agonists that regulate apoptosis. Materials and Methods : Sixty female Sprague-Dawley rats were anesthetized, and a laminectomy was performed at the 9th thoracic vertebra. The spinal cord injury was induced by dropping a 10 gm weight at a height of 20 mm. The subjects were divided into 5 groups. Group I was administered aminoguanidine, group II was administered GM-CSF, group III was administered riluzole, group IV was administered erythropoietin, and group V was administered methylprednisolone. A control group was administered normal saline. The results were assessed using the Tarlov motor grading method. In 1, 3, 5 and 7 days after the spinal cord injury, the rats were sacrificed and evaluated using the syringomyelic cavity size. Immunohistochemical staining for e-NOS and RT-PCR for XIAP were also performed. Results : Groups I, III, and V showed significantly different results in terms of the motor recovery and inhibition of increase in the syringomyelic cavity compared with the other groups (p 0.05). The e-NOS activity was observed in groups I, III, and V. The other groups showed almost no e-NOS activity. On the RT-PCR, groups I, III, and V showed significantly different results in terms of XIAP expression with time compared with the other groups. Conclusion : Steroids, NOS inhibitors and sodium channel inhibitors appear to be important factors for regulating apoptosis in the early stage of a spinal cord injury. Further study will be needed to develop new pharmaceuticals with synergic effects on spinal cord injuries.

목적: 쥐의 척수 손상 후 발현되는 사이토카인을 분석하여 세포 사멸을 조절하는 길항제에 대하여 알아보고자 하였다. 대상 및 방법: 평균 16주 된 Sprague-Dawley rat, 암컷 60마리를 사용하였으며, 9번 흉추에서 추궁절제 후 신경 손상을 유도한 후 생리식염수를 투여한 대조군 및 aminoguanidine을 투여한 I군, GM-CSF를 투여한 II군, riluzole을 투여한 III군, erythropoietin을 투여한 IV군, 그리고 methylprednisolone을 투여한 V군으로 분류하였다. 운동 측정은 Tarlov 점수를 이용하였고, 술 후 1일, 3일, 5일, 그리고 7일에 각각 쥐를 희생시켜 척수 공동의 크기, e-NOS에 대한 면역조직화학염색 및 XIAP에 대한 RT-PCR을 실시하였다. 결과: 운동 회복 및 척수 공동 증가 억제는 V, I, III군에서 다른 군에 비해 유의한 결과를 보였다(p 0.05). e-NOS 활성도의 발현은 I과 V, III군에서 저명하였으나, 나머지 군에서는 e-NOS 활성도의 발현은 거의 없었다. RT-PCR상 XIAP의 발현은 시간의 경과에 따라 V, I, III군에서 다른 군에 비해 강한 발현을 보였다. 결론: 척수 손상 후 초기에 세포 사멸을 조절하는 인자로 steroid, NOS 억제제, Na 통로 억제제 등이 중요할 것으로 사료되며, 추후 연구는 이들 약물들이 synergic effect를 일으킬 수 있는 약물의 개발에 중점을 두고자한다.

Keywords

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