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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Induction of Growth Hormone Release by Dioscin from Dioscorea batatas DECNE

  • Lee, Ho-Young (Department of Drug Research & Development, Korea Institute of Oriental Medicine) ;
  • Jung, Dae-Young (Department of Drug Research & Development, Korea Institute of Oriental Medicine) ;
  • Ha, Hye-Kyung (Department of Drug Research & Development, Korea Institute of Oriental Medicine) ;
  • Son, Kun-Ho (Department of Food and Nutrition, Andong National University) ;
  • Jeon, Su-Jin (Department of Food and Nutrition, Andong National University) ;
  • Kim, Chung-Sook (Department of Drug Research & Development, Korea Institute of Oriental Medicine)
  • Published : 2007.11.30
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Abstract

In this study, dioscin was isolated from Dioscoreae Rhizoma (DR), which is the rhizome of Dioscorea batatas DECNE. that inhabits broad areas of Korea and Japan. To determine whether dioscin induced growth hormone (GH) release, we evaluated its induction effects on GH release both in vitro and in vivo. The 70% methanol extract of DR, and its n-hexane and n-BuOH fractions, induced rat GH (rGH) release in rat pituitary cells 10-fold, 8-fold, and 5-fold higher than the control ($0.36{\pm}0.02 nM$), respectively (p < 0.05 each). The dioscin-induced rGH release of the cells was concentration-dependent and its $ED_{50}$ was $1.14{\times}10^{-5} M$. Within 90 minutes after intravenous administration of $10{\mu}g$/kg (p < 0.05 at $t_{max}$), dioscin caused the greatest increase in rGH concentration ($C_{max}$) in the rat plasma ($34.16{\pm}14.10 ng/ml$) (n = 4), which was twice as high as the control group ($12.88{\pm}3.29 ng/ml$) (n = 27).

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References

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