Laboraroty Animal Research

Korean Association for Laboratory Animal Science (한국실험동물학회)
권호 표지

Effect of 2,3,3',4,4'-Pentachlorobiphenyl (PCB#105) on Rat Testes

Won, Chung-Kil;Park, Oh-Sung;Han, Dae-Yong;Koh, Phil-Ok;Cho, Jae-Hyeon;Kim, Eun-Hee;Kim, Gon-Sup

  • Published : 20070900
⟨ Previous Next ⟩

Abstract

This study was conducted to examine the effect of 2,3,3',4,4'-pentachlorobiphenyl (PCB#105) on the reproductive function of male rats. PCB#105 (0.01, 0.1, 0.5 ㎍/㎏/day) in corn oil was orally administrated to adult male rats for 10 weeks. The body and testicular weights, and the serum levels of luteinizing hormone (LH) and follicle-stimulating hormone (FSH), were measured at 2, 4, 6, 8 and 10 weeks of PCB treatment. The morphological changes in the rat testes were than analyzed by light microscopy (LM) and transmission electron microscopy (TEM). The results showed that PCBs caused no significant change in the body weights but caused a marked decrease in their testicular weights. A decrease in the serum LH and FSH levels of the PCB-treated rats was also observed. Moreover, the morphological studies that were conducted on the PCB-treated rats revealed that the size of their seminiferous tubules slightly decreased (LM), and that their nuclear membrane was damaged when 0.1 ㎍/㎏/day PCB was administered to them for 10 weeks (TEM). These results suggest that the reproductive function of the adult male rats is sensitive to PCB, and that may affect their LH and FSH secretion.

Keywords

References

  1. Ahmad, N., Haltmeyer, G.C. and Eik-Nes, K.B. (1973) Maintenance of spermatogenesis in rats with intra-testicular implants containing testosterone or dehydrotestosterone (DHT). Biol. Reprod. 8, 411-419 https://doi.org/10.1093/biolreprod/8.4.411
  2. Backlin, B.M. and Bergman, A. (1995) Histopathology of postpartum placental sites in mink (Mustela vison) exposed to polychlorinated biphenyls or fractions thereof. APMIS 103(12), 843-854 https://doi.org/10.1111/j.1699-0463.1995.tb01443.x
  3. Bremner, W.J., Millar, M.R., Sharpe, R.M. and Saunders, P.T. (1994) Immunohistochemical localization of androgen receptors in the rat testis: evidence for stage-dependent expression and regulation by androgens. Endocrinology 135(3), 1227-1234 https://doi.org/10.1210/en.135.3.1227
  4. Brezner, E., Terkel, J. and Perry, A.S. (1984) The effect of Aroclor 1254 (PCB) on the physiology of reproduction in the female rat--I. Comp. Biochem. Physiol. 77(1), 65-70 https://doi.org/10.1016/0742-8413(84)90131-2
  5. Colborn, T., Vom Saal, F.S. and Soto, A.M. (1993) Developmental effects of endocrine-disrupting chemicals in wildlife and humans. Environ. Health Perspect. 101(5), 378-384 https://doi.org/10.2307/3431890
  6. Colborn, T.C.C. (1992) Chemically Induced Alterations in Sexual and Functional Development. In The Wildlife/Human Connection (Princeton, N.J. ed.), pp. ??-??, Princeton Scientific Publishing, Birmingham
  7. Crisp, TM., Clegg, ED., Cooper, RL., Wood, WP., Anderson, D.G., Baetcke, K.P., Hoffmann, J.L., Morrow, MS., Rodier, D.J. and Schaeffer, J.E. (1998) Environmental endocrine disruption, an effects assessment and analysis. Environ. Health Perspect. 106(1), 11-56 https://doi.org/10.2307/3433911
  8. Desaulniers. D., Leingartner, K., Wade, M., Fintelman, E., Yagminas, A. and Foster, W.G. (1999) Effects of acute exposure to PCBs 126 and 153 on anterior pituitary and thyroid hormones and FSH isoforms in adult Sprague Dawley male rats. Toxicol. Sci. 47(2), 158-169 https://doi.org/10.1093/toxsci/47.2.158
  9. Faqi, A.S., Dalsenter, P.R, Merker, H.J. and Chahoud, I. (1998) Effects on developmental landmarks and reproductive capability of 3,3',4,4'-tetrachlorobiphenyl and 3,3',4,4',5-pentachlorobiphenyl in offspring of rats exposed during pregnancy. Human Exp. Toxicol. 17(7), 365-372 https://doi.org/10.1191/096032798678908954
  10. Goldman, D. and Yawetz, A. (1992) The interference of polychlorinated biphenyls (Aroclor 1254) with membrane regulation of the activities of cytochromes P-450C21 and P-450(17) alpha,lyase in guinea-pig adrenal microsomes. J. Steroid Biochem. Mol. Biol. 42(1), 37-47 https://doi.org/10.1016/0960-0760(92)90009-8
  11. Gray, L.E., Ostby. J.S. and Kelce. W.R. (1997) A dose-response analysis of the reproductive effects of a single gestational dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin in male Long Evans Hooded rat offspring. Toxicol. Appl. Pharmacol. 146(1), 11-20 https://doi.org/10.1006/taap.1997.8223
  12. Hagenas, L., Ritzen, E.M., Ploen, L., Hansson, V., French, F.S. and Nayfeh, S.N. (1975) Sertoli cell origin of testicular androgen binding protein. Mol. Cell. Endocrinol. 2, 339-350 https://doi.org/10.1016/0303-7207(75)90021-0
  13. Hansen, L.G. (1998) Stepping backward to improve assessment of PCB congener toxicities. Environ. Health Perspect. 106(1), 171-189 https://doi.org/10.2307/3433919
  14. Hardy, M.P., Kelce, W.R., Klinefelter, G.R. and Ewing, L.L. (1990) Differentiation of Leydig cell precursors in vitro: a role for androgen. Endocrinology 127(1), 488-490 https://doi.org/10.1210/endo-127-1-488
  15. Heckert, L.L. and Griswold, M.D. (1991): Expression of folliclestimulating hormone receptor mRNA in rat testes and Sertoli cells. Mol. Endocrinol. Baltimore 5(5), 670-677 https://doi.org/10.1210/mend-5-5-670
  16. Kangasniemi. M., Kaipia, A., Toppari, J., Perheentupa, A., Huhtaniemi, I. and Parvinen, M. (1990) Cellular regulation of follicle-stimulating hormone (FSH) binding in rat seminiferous tubules. J. Androl. 11(4), 336-343
  17. Kim, I.S., Ariyaratne, H.B. and Chamindrani Mendis-Handagama, S.M. (2001) Effects of continuous and intermittent exposure of lactating mothers to aroclor 1242 on testicular steroidogenic function in the adult male offspring. Tissue Cell 33(2), 169-177 https://doi.org/10.1054/tice.2000.0168
  18. Kovacevic, R., Vojinovic-Miloradov, M., Teodorovic, I. and Andric, S. (1995) Effect of PCBs on androgen production by suspension of adult rat Leydig cells in vitro. Journal Steroid Biochem. Mol. Biol. 52(6), 595-597 https://doi.org/10.1016/0960-0760(95)00060-D
  19. Mably, T.A., Moore, R.W., Goy, R.W. and Peterson, R.E. (1992b) In utero and lactational exposure of male rats to 2,3,7,8-tetrachlorodibenzo-p-dioxin. 2. Effects on sexual behavior and the regulation of luteinizing hormone secretion in adulthood. Toxicol. Appl. Pharmacol. 114(1), 108-117 https://doi.org/10.1016/0041-008X(92)90102-X
  20. Mably, T.A., Moore, R.W. and Peterson, R.E. (1992a) In utero and lactational exposure of male rats to 2,3,7,8-tetrachlorodibenzo-pdioxin. 1. Effects on androgenic status. Toxicol. Appl. Pharmacol. 114(1), 97-107 https://doi.org/10.1016/0041-008X(92)90101-W
  21. Meachem, S.J., Wreford, N.G., Stanton, P.G., Robertson, D.M. and McLachlan, R.I. (1998) Follicle-stimulating hormone is required for the initial phase of spermatogenic restoration in adult rats following gonadotropin suppression. J. Androl. 19(6), 725-735
  22. Nishihara, M., Winters, C.A., Buzko, E., Waterman, M.R. and Dufau, M.L. (1988) Hormonal regulation of rat Leydig cell cytochrome P-45017 alpha mRNA levels and characterization of a partial length rat P-45017 alpha cDNA. Biochem. Biophysi. Res. Comm. 154(1), 151-158 https://doi.org/10.1016/0006-291X(88)90663-8
  23. Pflieger-Bruss, S., Hanf, V., Behnisch, P., Hagenmaier, H. and Rune, G.M. (1999) Effects ofsingle polychlorinated biphenyls on the morphology of cultured rat tubuli seminiferi. Andrologia 31(2), 77-82 https://doi.org/10.1111/j.1439-0272.1999.tb02849.x
  24. Roman, B.L., Timms, B.G., Prins, G.S. and Peterson, R.E. (1998) In utero and lactational exposure of the male rat to 2,3,7,8-tetrachlorodibenzo-p-dioxin impairs prostate development. 2. Effects on growth and cytodifferentiation. Toxicol. Appl. Pharmacol. 150(2), 254-270 https://doi.org/10.1006/taap.1998.8395
  25. Safe, S. (1990) Polychlorinated biphenyls (PCBs), dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), and related compounds: environmental and mechanistic considerations which support the development of toxic equivalency factors (TEFs). Crit. Rev. Toxicol. 21(1), 51-88 https://doi.org/10.3109/10408449009089873
  26. Sager, D.B. (1983) Effect of postnatal exposure to polychlorinated biphenyls on adult male reproductive function. Environ. Res. 31(1), 76-94 https://doi.org/10.1016/0013-9351(83)90063-4
  27. Sager, D.B., Shih-Schroeder, W. and Girard, D. (1987) Effect of early postnatal exposure to polychlorinated biphenyls (PCBs) on fertility in male rats. Bull. Environ. Contam. Toxicol. 38(6), 946-953 https://doi.org/10.1007/BF01609078
  28. Shain, W., Overmann, S.R., Wilson, L.R., Kostas, J. and Bush, B. (1986) A congener analysis of polychlorinated biphenyls accumulating in rat pups after perinatal exposure. Arch. Environ. Contami. Toxicol. 15(6), 687-707 https://doi.org/10.1007/BF01054916
  29. Takagi, Y., Aburada, S., Hashimoto, K. and Kitaura, T. (1986) Transfer and distribution of accumulated (14C)polychlorinated biphenyls from maternal to fetal and suckling rats. Arch. Environ. Contami. Toxicol. 15(6), 709-715 https://doi.org/10.1007/BF01054917
  30. Tang, P.Z., Tsai-Morris, C.H. and Dufau, M.L. (1998) Regulation of 3beta-hydroxysteroid dehydrogenase in gonadotropin-induced steroidogenic desensitization of Leydig cells. Endocrinology 139(11), 4496-4505 https://doi.org/10.1210/en.139.11.4496
  31. Van den Berg, M., Birnbaum, L., Bosveld, A.T., Brunstrom, B., Cook, P., Feeley, M., Giesy, J.P., Hanberg, A., Hasegawa, R. and Kennedy, S.W. (1998) Toxic equivalency factors (TEFs) for PCBs, PCDDs, PCDFs for humans and wildlife. Environ. Health Perspect. 106(12), 775-792 https://doi.org/10.2307/3434121
  32. Wilker, C., Johnson, L. and Safe, S. (1996) Effects of developmental exposure to indole-3-carbinol or 2,3,7,8-tetrachlorodibenzo-pdioxin on reproductive potential of male rat offspring. Toxicol. Appl. Pharmacol. 141(1), 68-75 https://doi.org/10.1016/S0041-008X(96)80010-X
  33. Yamamoto, M., Shirai, M., Tamura, A., Kobayashi, T., Kohara, S., Murakami, M. and Arishima, K. (2005) Effects of maternal exposure to a low dose of diethylstilbestrol on sexual dimorphic nucleus volume and male reproductive system in rat offspring. J. Toxicol. Sci. 30, 7-18 https://doi.org/10.2131/jts.30.7