Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지

Genetic Relationships of Korean Astringent Persimmon Varieties Using AFLP Analysis

AFLP 분석을 통한 국내 떫은감의 유연관계 분석

Cho, Doo-Hyun;Chun, Ik-Jo;Kwon, Soon-Tae;Song, Ye-Su;Chou, Yoen-Dae
조두현;전익조;권순태;송예수;추연대

  • Published : 2007.06.30
⟨ Previous Next ⟩

Abstract

Astringent type persimmon varieties have been cultivated from ancient times in Korea. In the long cultivation of the persimmon, various local varieties have been developed and cultivated throughout from the central to the Southern area. Therefore, the aim of this study was to reveal genetic relationships of local 22 persimmon varieties, including one Chinese cultivar ‘Damopan’ and four Japanese cultivar ‘Koshuhyakume’, ‘Hirataneanshi’, ‘Saijo’, and ‘Yokono’, using AFLP technique. Total bulk DNA of the persimmon cultivars were amplified with primer combinations based on the sequence of EcoRI and MseI adapters. The eight primer combinations amplified a total of 357 polymorphic fragments. According to AFLP calculated genetic similarities, ‘Godongsi’ and ‘Hakdongsi’ were closely related each other. In addition, ‘Durigam’ and ‘Yokono’ showed close genetic similarity, which were collected from Hamyang in Korea and Japan, respectively. However, Chinese persimmon ‘Dampon’ and ‘Wolhasi’ were poorly related with the other collected varieties.

Keywords

References

  1. Baird, W.V., R.E. Ballard, S. Rajapakse, and A.G. Abbott. 1996. Progress in Prunus mapping and application of molecular markers to germplasm improvement. HortScience 31: 10991106
  2. Barrete, B.A. and K.K. Kidwell. 1998. AFLP-based genetic diversity assesment among wheat cultivars from Pacific Northwest. Crop. Sci. 38:1261-271 https://doi.org/10.2135/cropsci1998.0011183X003800050025x
  3. Cho, D.H. 2005. Classification of Korean domestic persimmon varieties (Diospyros kaki Thunb.) using morphological and genetical characteristics. Andong Nat'l Univ. Ph.D. Disser. (in Korean)
  4. Cho, S.K. and T.H. Cho. 1965. Studies on the local varieties of persimmon in Korea (in Korean with English summary). Res. Rep. RDA 8:147-190
  5. Dirlewanger, E., A. Moing, C. Rothan, L. Svanella, V. Pronier, A. Guye, C. Plomion, and R Monte. 1999. Mapping QTLs controlling fruit quality in peach (Prunus persica (L.) Batsch). Theor. Appl. Genet. 98: 18-31 https://doi.org/10.1007/s001220051035
  6. Hagidimitriou, M., A. Katsiotis, G. Menexes, C. Pontikis, and M. Loukas. 2005. Genetic diversity of major Greek olive cultivars using molecular (AFLPs and RAPDs) markers and morphological traits. J. Amer. Sco. Hort. Sci. 130:21-217
  7. Helentijaris, T., G. King, M. Slocum, D. Siedenstang, and S. Wegman. 1985. Restriction fragment length polymorphisms as probes for plant diversity and their development as tools for applied plant breeding. Plant Mol. BioI. 5: 109-118 https://doi.org/10.1007/BF00020093
  8. Kim, T.C. 1993. Taxonomic studies of persimmon (Diospyros kaki Thunb.) by multivariate and isozyme analyses. Seoul Nat'l. Univ. Ph.D. Disser. (in Korean)
  9. Kumar, L.S. 1999. DNA markers in plant improvement: An overview. Biothehnol. Adv. 17:143-182 https://doi.org/10.1016/S0734-9750(98)00018-4
  10. Kwon, S.J., S.N. AIm, H.C. Hong, Y.K Kim, H.G. Hwang, H.C. Choi, and H.P. Moon. 1999. Genetic diversity of Korean japonica rice cultivars. Kor. J. Breed. 31:268-275. (in Korean)
  11. Kwon, Y., J. Moon, Y. Kwon, D. Park, W. Yoon, I. Song, and S. Yi. 2003. AFLP analysis for cultivar discrimination in radish and chinese cabbage. Kor. J. Breed. 35:319-328. (in Korean)
  12. Lu, Z.X., K Sossey-Alaoui, G.L. Reighard, W.V. Baird, and A.G. Abbott. 1999. Development and characterization of a codominant maker linked to root-knot nematode resistance, and its application to peach rootstock breeding, Theor. Appl. Genet. 99:115-122 https://doi.org/10.1007/s001220051215
  13. Oraguzie, N.C., S.E. Gardiner, H.C.M. Basset, M. Stefanati, RD. Ball, V.G.M. Bus, and A.G. White. 2001. Genetic diversity and relationships in Malus sp. germplasm collections as determined by randomly amplified polymorphic DNA. J. Amer. Soc. Hort. Sci. 126:318-328
  14. Prado, M.J., M.T. Herreara, R.A. Vazquez, S. Rome, and M.V. Gonzalez. 2005. Micropropagation of two selected male kiwifruit and analysis of genetic variation with AFLP markers. HortScience 40:740-746
  15. Song, Y., L. Hepting, G. Schweizer, L. Hartl, G. Wenzel, and A. Schwarzfischer. 2005. Mapping of extreme resistance to PVY ($RY_{sto}$) on chromosome XII using anther-culture-derived primary dihaploid potato lines. Theor, Appl. Genet. 111:879-887 https://doi.org/10.1007/s00122-005-0010-7
  16. Vos P., R. Hougers, M. Bleeker, M. Reijans, T. Vand de Lee, M. Homes, A. Frijters, J. Pot, J. Peleman, M. Kuiper, and M. Zabeau. 1995. AFLP: A new technique for DNA fingerprinting. Nucleic Acids Research 23:4407 -4414 https://doi.org/10.1093/nar/23.21.4407
  17. Williams, M.N.V., N. Pande, S. Nair, M. Mohan, and J. Bennett. 1991. Restriction fragment length polymorphism analysis of polymerase chain reaction products amplified from mapped loci of rice (Oryza sativa L.) genomic DNA. Theor. Appl. Genet. 82:489-498 https://doi.org/10.1007/BF00588604
  18. Yang, D.S. 1998. Researches on the distribution, genetic relation, and characteristics of local persimmon varieties grown in Jeollabuk-do. Jeju National University. Ph.D. Disser. (in Korean)