Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Postnatal Expression Pattern of Adipose Type Fatty Acid Binding Protein in Different Adipose Tissues of Porcine

  • Xu, C.L. (Institute of Feed Science, the Key Laboratory of Molecular Animal Nutrition, Ministry of Education Zhejiang University) ;
  • Wang, Y.H. (Institute of Feed Science, the Key Laboratory of Molecular Animal Nutrition, Ministry of Education Zhejiang University) ;
  • Huang, Y.H. (Institute of Feed Science, the Key Laboratory of Molecular Animal Nutrition, Ministry of Education Zhejiang University) ;
  • Liu, J.X. (Institute of Feed Science, the Key Laboratory of Molecular Animal Nutrition, Ministry of Education Zhejiang University) ;
  • Feng, J. (Institute of Feed Science, the Key Laboratory of Molecular Animal Nutrition, Ministry of Education Zhejiang University)
  • Received : 2006.10.05
  • Accepted : 2007.01.04
  • Published : 2007.06.01
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Abstract

Adipocyte fatty acid-binding protein (A-FABP), which belongs to the FABP family, plays an essential role in long-chain fatty acid uptake and metabolic homeostasis, especially in adipose tissue. The pattern of A-FABP gene mRNA expression in different growth stages and its relation to intramuscular fat (IMF) accretion in pigs was studied. Fifteen female $Duroc{\times}Landrace{\times}Yorkshire$ pigs in five groups of three pigs each, weighing 1, 30, 50, 70 and 90 kg were used to study developmental gene mRNA expression of A-FABP in various adipose tissues by means of semi-quantitative RT-PCR. Results showed that A-FABP mRNA levels in subcutaneous and ventral adipose tissues first increased from 1 to 50 kg, then gradually declined from 50 to 90 kg. Moreover, the rank order of A-FABP mRNA levels determined in three adipose tissues was as follows: subcutaneous adipose>ventral adipose>mesenteric adipose. A-FABP mRNA expression in mesenteric adipose tissue was constant during development. In addition, a positive correlation from 1 to 50 kg BW pigs and a negative correlation from 50 to 90 kg BW between A-FABP mRNA levels in subcutaneous and ventral adipose and IMF content were found.

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