Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Effects of Cryoprotectants and Diluents on Cryopreservation of the Red Seabream, Pagrus major Sperm

참돔, Pagrus major 정자의 동결보존에 미치는 희석제와 동해방지제의 효과

  • Kho, Kang Hee (Faculty of Marine Technology, Chonnam National University)
  • 고강희 (전남대학교 수산해양대학 해양기술학부)
  • Received : 2007.04.02
  • Accepted : 2007.05.14
  • Published : 2007.06.30
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Abstract

A series of experiments were conducted to compare the effects of various diluents and cryoprotectants on the motility and survival rate in cryopreservation of the red seabream, Pagrus major sperm. Sperm was efficiently cryopreserved using 300 mM glucose as a diluent. Two cryoprotectant, dimethyl sulfoxide (DMSO) and glycerol, were added to 300 mM glucose to formulate the extenders at concentrations between 5% and 30% by volume for freezing. The highest post-thawed sperm motility and survival rate were obtained with 10% DMSO.

참돔 정자의 동결보존에 미치는 희석제와 동해방지제의 효과를 운동성과 생존율로 비교하였다. 300 mM glucose를 희석제로 사용하였을 때 보존 효과가 좋았다. 동해방지제로는 10% dimethyl sulfoxide (DMSO)를 동결보존에 사용하였을 때 해동 후의 운동성과 생존율이 가장 높았다.

Keywords

References

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