Horticulture, Environment, and Biotechnology : HEB

Korean Society of Horticultural Science (한국원예학회)
권호 표지

The Use of Molecular Markers to Certify Clubroot Resistant Cultivars of Chinese Cabbage

Piao, Zhong Yun;Choi, Su-Ryun;Lee, Young-Mi;Kim, Hong-Gi;Lim, Yong-Pyo

  • Published : 2007.06.10
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Abstract

Chinese cabbage cultivated in most area of Korea is subjected to the most serious clubroot disease caused by Plasmodiophora brassicae Wor. A number of clubroot resistant (CR) cultivars were released or in development. We have previously tagged CR locus (CRb) within 3 cM flanked by two co-dominant markers (TCR01 and TCR05) and one dominant marker (TCR09). Thirty-seven CR cultivars and ten non-CR cultivars were collected to study the utility of these DNA markers. In addition, markers (BRMS096, BRMS088, and OPC11-2S) linked to Crr1, Crr2, and Crr3, respectively, were evaluated. Of the surveyed CR cultivars, 18 carried marker fragment TCR01₂₀₀ and TCR05₂₇₉ linked to CRb in coupling. Evaluation of these cultivars to race 4 of clubroot revealed that all of them conferred resistance. Other CR cultivars absent of TCR01₂₀₀ and TCR05₂₇₉ were either resistant or susceptible. Only OPC11-2S linked to Crr3 can explain the two CR cultivars showing susceptible, while others cannot. Analysis of European Clubroot Differential set 01 to 04 indicate that marker fragment TCR05₂₇₉ with the CRb locus, possibly also TCR01₂₀₀, was introgressed from CR turnip into partial CR cultivars of Chinese cabbage. The usefulness of these markers in marker-assisted selection was also discussed.

Keywords

References

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