Journal of the Korean Society of International Agriculture (한국국제농업개발학회지)

The Korean Society of International Agriculture (한국국제농업개발학회)
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Antioxidant and Anticancer Activities of Extracts from Grains of the Barley Germplasms

보리 유전자원 종실 추출액의 항산화 및 항암 활성

Lee, Dong-Jin;Kim, Heon-Woong;Park, Sang-Gu;Chu, Sang-Mi;Lee, Jae-Sung
이동진;김헌웅;박상구;추상미;이재성

  • Published : 20070900
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Abstract

DPPH(1, 1-diphenyl-2-picrylhydrazyl) free radical scavenging activity assay and MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium) assay were used to analyze antioxidant and anticancer activities in 405 barley germplasms. In more than antioxidant activities(70%), the covered and naked barley having colors showed similar distribution of 28(14.5%) and 8(15.1%) accessions whereas the covered and naked barley landraces are 3(2.9%) and 14(25%) accessions, respectively. Against B16-F10 mouse melanoma cell lines the covered and naked barley landraces are showed distribution of 0-70% and 0-58%, respectively. And all the covered and naked barley having colors showed distribution of 0-70%. In antioxidant activities of native and colored barley, the covered and naked barley are showing a normal distribution in the center of 10-50%, In anticancer activities naked barley showed the tendency of distribution lowly as activities are higher whereas the covered barley are showing a normal distribution in the center of 10-50%.

보리 유전자원 405점에 대해 항산화 및 항암 활성을 검정한 결과는 다음과 같다. 1. 70% 이상의 항산화 활성에서 재래종 겉보리 및 쌀보리는 각각 103점 중 3점(2.9%), 56점 중 14점(25%)으로 쌀보리에서 활성이 높은 품종이 더 많이 조사되었다. 반면에 유색종 겉보리 및 쌀보리는 각각 193점 중 28(14.5%), 53점 중 8점(15.1%)의 비슷한 분포를 나타냈다. 2. 쥐 흑색종 세포인 B16-F10 cell lines에 대하여 재래종 겉보리 및 쌀보리는 각각 활성이10% 이하부터 70% 미만, 10% 이하부터 58% 미만인 분포를 나타냈으며, 유색종 겉보리 및 쌀보리는 모두 10% 이하에서 70% 미만까지의 분포를 나타냈다. 3. 재래종 및 유색종 보리의 항산화 활성에서 겉보리 및 쌀보리는 10-50%의 활성을 중심으로 정규 분포를 나타내고 있으며, 항암 활성에서는 겉보리의 경우 10-50%의 활성을 중심으로 정규 분포를 보이는 반면, 쌀보리의 경우 활성이 높아질수록 낮게 분포하는 경향을 보이고 있다.

Keywords

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