Journal of Korean Academy of Oral Health

The Korean Academy of Preventive Dentistry and Oral Health (대한예방치과·구강보건학회)
권호 표지

Improvement effect of oral environment after using mouthwash containing cetylpyridinium chloride or triclosan

Cetylpyridinium chloride 및 Triclosan을 배합한 양치액의 구강환경 개선효과에 관한 연구

Choi, Jae-Myung;Choi, Ji-Young;Hwang, Kyung-Sook;Park, Yong-Duk
최재명;최지영;황경숙;박용덕

  • Published : 2007.09.30
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Abstract

Objectives: The aim of this study was to investigate the effects of tea tree oil on apoptosis KB cell by the use of in vitro cytotoxicity assay, Nuclear fragmentation assay with Hoechst staining, and DNA fragmentation analysis by gel electrophoresis. Methods: KB cells, human squamous cell carcinoma cell line (KCLB 10017; Korean Cell Line Bank, South Korea) were grown. and Human gingivo-fibroblast tissue of extracted tooth was taken sample tissue in 1×HBSS (with 3×antibiotics) from oral surgery clinic. Tea-tree oil was provided by Korean Plantation Redpond Herb Farm, Je-ju, South Korea. Appropriate concentrations range of 0.001-0.1 (v/v) were set up in DMEM. In the cytotoxicity assay, survival rate of KB and HGF cells were determined by MTT assays, as described. For the nuclear fragmentation assay with Hoechst staining, morphological assessment of the apoptotic cells was performed using the Hoechst 33342 staining. For the DNA fragmentation analysis by gel electrophoresis, DNA purification process was performed by DNA Purification Kit (Promega, USA). Results: MTT assays showed significant differences at the level of 0.01, 0.05, 0.1 (v/v) concentration between KB cell and HGF cell (p<0.05). Nuclear fragmentation assay with Hoechst staining showed in tea-tree oil 0.05 (v/v) concentration for 12 hours. DNA fragmentation analysis by gel electrophoresis showed DNA fragmentation in 0.1 (v/v) concentration for 6 hours, 0.05 (v/v), 0.1 (v/v) for 12 hours, and 0.01 (v/v) 24 hours. Conclusions: Through this study, tea-tree essential oil is regarded to protect human gingivo-fibroblast cell and but to lead apoptosis of KB cell.

Keywords

References

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