Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지

Molecular Genetics of Brassicaceae Based on AFLP Display

AFLP 분석을 이용한 배추과 식물의 종속간 분자유전연구

Kwon, Soo-Jin;Lim, Ki-Byung;Lim, Myung-Ho;Park, Jee-Young;Kim, Jin-A;Kim, Jung-Sun;Lee, Soo-Seong;Park, Beom-Seok;Jin, Yong-Moon
권수진;임기병;임명호;박지영;김진아;김정선;이수성;박범석;진용문

  • Published : 2007.06.30
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Abstract

Genetic diversity and relationship in the related plant taxa is a prerequisite for crop improvement. Amplified fragment length polymorphism (AFLP) fingerprinting is one of the powerful and widely used tools, which has found wide spread application in numerous studies ranging from genetic mapping to diversity surveys in plants and other organisms. Genome relationship among diploid and allotetraploid Brassica taxa, and between interspecific hybrid or intergeneric hybrid and their parental species were investigated using AFLP display. The two primer pairs for AFLP reactions and seven EcoRI (+2) and M5eI (+3) primer combinations were used in the measurement of genetic relationships. Genetic similarities among nine accessions were estimated by Nei’s distance equation, and cluster analysis was performed using the unweighed pair group method (UPGMA) in the computer program NTSYS-pc. One intergeneric hybrid between Brassica and Raphanus showed 2n=4x=38 chromosomes, of which Raphanus chromosome segment were translocated with B. rapa chromosome.

Keywords

References

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