Horticulture, Environment, and Biotechnology : HEB

Korean Society of Horticultural Science (한국원예학회)
권호 표지

Degree of Abortion and Germination Percentage in Seeds Derived from Interploid Crosses between Diploid and Tetraploid Grape Cultivars

Heo, Jae-Yun;Park, Kyo-Sun;Yun, Hae-Keun;Park, Sung-Min

  • Published : 2007.04.10
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Abstract

This experiment was conducted to demonstrate the degree of seed abortion and germination percentage in seeds derived from interploid crosses between diploid and tetraploid grape cultivars as the first step for triploid breeding. In the crosses between three diploid and six tetraploid grape cultivars, a total of 11,015 pollens was pollinated and percentage of seed set was 21.7% on average. In the crosses between five tetraploid and twelve diploid grape cultivars, a total of 9,107 pollens were pollinated and percentage of seed set was 9.0% on average. In 2x × 4x and 4x × 2x crosses, the frequency of sinker seeds reflecting the presence of embryo and endosperm was 71.2 (2,551 seeds) and 58.7% (603 seeds), respectively. The sinker seeds derived from 2x × 4x and 4x × 2x crosses were treated with low temperature, and then were sowed for determining germination rates. In 2x and 4x crosses, 194 seeds were germinated and the percentage of seed germination was 7.6% on average. In 4x and 2x crosses, 77 seeds were germinated and the percentage of seed germination was 12.8% on average. The percentages of seed germination were not different between the 4x × 2x and 2x × 4x crosses. Chromosome number observation in root tip cells indicated that all seedlings obtained from 2x × 4x and 4x × 2x crosses were triploid plants with 57 chromosomes. In the 2x × 4x and 4x × 2x crosses, most seeds derived from crosses did not have perfectly developed endosperm. Although frequency of seed with embryo in interploid crosses was high, cause of low germination percentage was judged by the result of regeneration of embryo.

Keywords

References

  1. Brink, R.A. and D.C. Cooper. 1947. The endosperm in seed development. Bot. Rev. 13:423-541 https://doi.org/10.1007/BF02861548
  2. Cain, D.W., R.L. Emershad, and R.E. Tarailo. 1983. In-ovulo embryo cultrue and seedling development of seeded and seedless grape (Vilis vinifera L.). Vitis 22:9-14
  3. Ehlenfeldt, M.K. and R.E. Hanneman. 1988. Genetic control of endosperm balance number (EBN) three additive loci in a threshold-like system. Theor. Appl. Genet. 75:825-832
  4. Einset, J. and C. Pratt. 1975. Grapes, p. 130-153 In: J. Janick and J.N. Moore (eds.). Advances in fruit breeding. Purdue Univ. Press, West Lafayette, IN, USA
  5. Emershad, R.L., D.W. Ramming, and M.D. Serpe. 1989. In ovulo embryo development and plant formation from stenospermic genotypes of Vitis vinifera. Amer. J. Bot. 76:397-402 https://doi.org/10.2307/2444609
  6. Esen, A. and R.K. Soost. 1973. Seed development in Citrus with special reference of 2x ${\times}$ 4x crosses. Amer. J. Bot. 60:448-462 https://doi.org/10.2307/2441501
  7. Esen, A., R.K. Soost, and G. Gerace. 1978. Seed set, size, and development after 4x${\times}$2x and 4x ${\times}$ 4x crosses in Citrus. Euphytica 27:283-294 https://doi.org/10.1007/BF00039144
  8. Goldy, R., R. Emershad, D.W. Ramming, and J. Chaparo. 1988. Embryo culture as a means of introgressing seedlessness from Vitis vinifera to V. rotundifolia. HortScience 23:886-889
  9. Gribaudo, I., R. Zanetti, R. Botta, R. Vallania, and I. Eynard. 1993. In ovulo embryo culture of stenospermocarpic grapes. Vitis 32:9-14
  10. Johnston, S.A. and R.E. Hanneman. 1996. Genetic control of endosperm balance number (EBN) in the Solanaceae based on trisomic and mutation analysis. Genome 39:314-321 https://doi.org/10.1139/g96-042
  11. Johnston, S.A. and R.E. Hanneman. 1999. The nature of the genetic control of endosperm balance number based on aneuploid analysis of Datura. Sex. Plant. Rpt. 12:71-75 https://doi.org/10.1007/s004970050174
  12. Johnston, S.A., T.P.M. den Nijs, S.J. Peloquin, and R.E. Hanneman. 1980. The significance of genic balance to endosperm development in interspecific crosses. Theor. Appl. Genet. 57:5-9 https://doi.org/10.1007/BF00276002
  13. Kim, J.H., B.J. Ham, D.J. Lim, and K.C. Lee. 1996a. Cross compatibility seed germinability, and embryo rescue following 2x ${\times}$ 4x crosses in Hibiscus syriacus. J. Kor. Soc. Hort. Sci. 37:713-718
  14. Kim, J.H., R.J. Ham, H.T. Lim, and K.C. Lee. 1996b. Development of immature embryo and abnormal endosperm after reciprocal crosses between diploids and tetraploids in Hibiscus syriacus. J. Kor. Soc. Hort. Sci. 37:462-467
  15. Liu, S.M., S.R. Sykes, and P.R. Clingeleffer. 2003. Improved in ovulo embryo culture for stenospermocarpic grapes (Vitis vinifera L.). Austral. J. Agr. Res. 54:869-876 https://doi.org/10.1071/AR03053
  16. Park, S.M., A. Wakana, M. Hiramatsu, and K. Uresino. 2002. A tetraploid hybrid plant from 4x ${\times}$ 2x crosses in Vitis and its origin. Euphytica 126:345-353 https://doi.org/10.1023/A:1019931502788
  17. Park, S.M. and J.H. Kim. 2001. Development of endosperm and embryo of aneuploid seeds obtained from 3x ${\times}$ 2x and 3x ${\times}$ 4x crosses and its rescue in grapes. J. Kor. Soc. Hort. Sci. 42:315-318
  18. Sanford, J.C. 1983. Ploidy manipulations, p. 100-123 In: J.N. Moors and J. Janick (eds.), Methods in fruit breeding. Purdue Univ. Press, West Lafayette, IN, USA
  19. Wakana, A., M. Hiramatsu, S.M. Park, N. Hanada, I. Fukudome, and B.X. Ngo. 2002. Degree of abortion and germination rates in triploid seeds from crosses between diploid and tetraploid grapes (Vitis vinifera L. and V. complex). J. Fac. Agr. Kyushu Univ. 46:281-294
  20. Wakana, A., M. Hiramatsu, S.M. Park, N. Hanada, I. Fukudome, and K. Yasukochi. 2003. Seed abortion in crosses between diploid and tetraploid grapes (Vitis vinifera and V. complex) and recovery of triploid plants through embryo culture. J. Fac. Agr. Kyushu Univ. 48:39-50
  21. Wakana, A., S.M. Park, M. Hiramatsu, N. Hanada, I. Fukudome, and K. Yasukochi. 2005. Characteristics of seedless berries of triploid hybrid grape from reciprocal crosses between diploid 'Muscat Bailey A' and tetraploid 'Red Pearl'. J. Fac. Agr. Kyushu Univ. 50:49-59
  22. Yamashita, H., I. shigehara, and H. Haniuda. 1998. Production of triploid grapes by in oval embryo culture. Vitis 37:113-117
  23. Yamashita, H., S. Horicuchi, and T. Taira. 1993. Development of seeds and the growth of triploid seedlings obtained from reciprocal crosses between diploids and tetraploids grapes. J. Jpn. Soc. Hort. Sci. 63:249-255