Horticulture, Environment, and Biotechnology : HEB

Korean Society of Horticultural Science (한국원예학회)
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Identification of Zygotic and Nucellar Mandarin Seedlings Using Randomly Amplified Polymorphic DNA

Yun, Jin-Ung;Yang, Hee-Beom;Jung, Yong-Hwan;Yun, Su-Hyun;Kim, Kwang-Sik;Kim, Chan-Shick;Song, Kwan-Jeong

  • Published : 2007.06.10
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Abstract

The study aimed at evaluating an efficiency of cross breeding in ‘Miyagawa Wase’ and ‘Okitsu Wase’ satsuma mandarin and ‘Shiranuhi’ mandarin through investigating the seed and seedlings formation, the frequency and position of zygotic seedlings, and the influence of pollen parents using randomly amplified polymorphic DNA (RAPD). Seedlings were in vitro cultured with seeds obtained from four crosses of ‘Miyagawa Wase’ (Citrus unshiu) × ‘Ponkan’ (C reticulata), ‘Okitsu Wase’ (C unshiu) × ‘Swingle Citrumelo’ (C. paradisi × Poncirus trifoliate), ‘Shiranuhi’ [(C unshiu × C. sinensis) × C. reticulata] × ‘Ponkan’ (C. reticulata), and ‘Shiranuhi’ [(C. unshiu × C. sinensis) × C. reticulata] × ‘Swingle Citrumelo’ (C. paradisi × P. trifoliate). RAPD analysis was conducted with five primers selected from screening 77 primers based on DNA amplification of parent plants. Seed formation including total seeds and full sized seeds per fruit between ‘Miyagawa Wase’ and ‘Okitsu Wase’ was not different statistically. However, seed formation of ‘Shiranuhi’ mandarin was significantly affected by pollen genotype. Average seedlings number per seed was not different effectively. The frequency and positioning patterns of zygotic seedling were different in each cultivar. The results suggest that seed formation, polyembryony, and frequency and positioning of zygotic seedling in some citrus having polyembryony might be affected by seed and pollen parents.

Keywords

References

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