Laboraroty Animal Research

Korean Association for Laboratory Animal Science (한국실험동물학회)
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Comparison with 3 High-Fat Diet for Studying Obesity in C57BL/6 Mouse

Yun, Jun-Won;Lee, Byoung-Seok;Kim, Chae-Wook;Kim, Bae-Hwan

  • Published : 20070900
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Abstract

Obesity is an abnormal condition in adipose tissue, which is the main site of lipid storage and plays a critical role in the regulation of energy balance. Although obesity is caused by various environmental and genetic factors, the consumption of high-fat diet (HFD) is thought to be one of the main factors. This study was designed to evaluate 3 HFD in C57BL/6 mouse for studying obesity. The final body weight in all HFD groups was higher than those in all control groups. And, all HFD groups had a significant increment of epididymal fat pad weights than all control groups. Therefore, all 3 HFD in model of diet-induced obesity are useful diets for studying obesity. However, when it was compared with ALH (23.9% HFD supplemented with lard) group and ASH (40% HFD supplemented with shortening) group, DH (23.6% HFD supplemented with lard) group had significant increment of body weight in the early stage of treatment, and markedly showed high increment rate of body weight. And, AC group, control group, showed increasing tendency in serum AST levels and revealed slight altered liver structure with vacuolation disseminated in the portal space. This result may be correlation with slight accumulation of fat due to AIN93G diet itself intake. Consequently, considering results of body weight and epididymal fat weight, 3 HFD are useful diets for diet-induced obesity model. However, 23.6% HFD supplemented with lard (DH group) is better choice for obesity studies with laboratory rodents.

Keywords

References

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