Molecular & Cellular Toxicology

The Korean Society of Toxicogenomics and Toxicoproteomics (대한독성유전 단백체학회)
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Toxicoproteomic Analysis of Differentially Expressed Proteins in Rat Liver by DEHP

  • Son, Bu-Soon (Department of Environmental Health Scinece, SoonChunHyang University) ;
  • Seong, Ah-Reum (Department of Biotechnology, SoonChunHyang University) ;
  • Park, Seul-Ki (Department of Biotechnology, SoonChunHyang University) ;
  • Kim, Wan-Jong (Department of Life Science, SoonChunHyang University) ;
  • Ryu, Jae-Chun (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and Technology) ;
  • Lee, Mi-Young (Department of Biotechnology, SoonChunHyang University)
  • Published : 2007.12.31
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Abstract

The endocrine disrupting chemical, di (2-ethylhexyl) phthalate (DEHP) is a plasticizer used in polyvinyl chloride products ubiquitous in our daily lives. DEHP has potentially adverse effects on the liver, kidney, lung, heart, reproductive organs and endocrine systems. Many toxicological data on the DEHP toxicity have been stated, but complete protein profiles have not yet been reported. In this study, DEHP-induced oxidative DNA damage in rat lymphocyte was evaluated by Comet assay (single-cell gel electrophoresis) for the first time. Moreover, DEHP-induced protein profile alterations were examined in rat liver by using toxicoproteomic tools. 34 protein spots in the liver were identified to be significantly deregulated by DEHP on the 2-dimensional gel. Among them, 20 spots were up-regulated and 14 spots down-regulated by DEHP.

Keywords

References

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