Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Effect of Glycerol Concentration, Freezing Rate and Thawing Rate on Semen Characteristics in PoongSan-dog

풍산개 정자의 동결보존에 있어서 Glycerol 농도, 동결 및 융해속도가 정자성상에 미치는 영향

  • Ji, D.Y. (Seoul Grand Park Zoo) ;
  • Yoon, T.C. (Korea University Institute of life Science and Natural Resources) ;
  • Rho, J.R. (Seoul Grand Park Zoo) ;
  • Cho, S.R. (National Institute of Animal Science, RDA) ;
  • Kim, C.K. (Department of Animal Science & Technology, Chung-Ang University) ;
  • Pang, M.G. (Department of Animal Science & Technology, Chung-Ang University) ;
  • Kim, Bo-Sook (Seoul Grand Park Zoo)
  • Published : 2007.10.31
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Abstract

This research was carried out in order to establish the production technique for Poong-san dog’s frozen semen, by examining the semen characteristic and the volume of glycerol added to the dilution solution, thawing temperature and sperm motility and viability as well as the motility using CASA according to time variation. Average semen volume was 5.9ml, sperm concentration 116.3×106 sperm/ml, total sperm number 789.3×106 sperm, motility 88.7±1.7% and viability 87.6±7.8%. When it was cryopreservation and thawed at different glycerol concentrated extender, it showed 52.7% motility and 57.7±10.3% viability at 7% glycerol, compared to other treatments. For semen cryogeny, at conditions of 5, 7cm and a height of 10cm for pre-cryogeny and maintaining the semen at 7cm from the surface of liquid nitrogen resulted in profitable motility and viability.

본 연구는 풍산개 동결정액 제조기술을 정립하기 위하여 정액성상과 정액 동결 시 희석액에 첨가되는 Glycerol 농도, 동결속도, 융해온도와 시간에 따라 정액의 운동성과 생존율 및 CASA를 이용한 운동성 등에 대하여 조사하여 최적의 동결조건을 확립하기 위해 실시하였다.1.풍산개의 평균 정액량 5.9ml, 정액농도 116.3 ×106sperm/ml 총정자수 789.3×106sperm, 운동성 88.7±1.77% 및 생존율 87.6±1.85% 였다. 2.1차 희석액을 상온에서 희석 후 상온에서 4°C까지 하강시킨 후 glycerol 농도가 3%, 5% 및 7% 첨가된 2차 희석액을 희석 후 6일간 운동성을 측정한 결과 5일째 3%일 때 46.2±9.3%, 5% glycerol에서는 48.1±8.5% 및 7%일 때 52.7±8.2%로 glycerol 7%가 유의적으로 높은 운동성을 나타냈다.3. 각기 다른 glycerol 농도를 함유한 동결보존액에서 동결보존 후 융해하였을 때 7%의 glycerol 농도에서 각각 52.7%와 57.7±10.3%로서 다른 처리구에 비해 유의적인(P<0.01) 운동성 및 생존율을 나타냈다.4.정액을 동결함에 있어 예비동결시 57 및 10cm의 높이에서 정치시킨 후 동결을 실시하였을 때 액체질소의 표면 7cm의 높이에서 동결을 실시한 처리구에서 전체적으로 유의한 운동성과 생존율을 나타냈다.

Keywords

References

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