Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지

Simultaneous determination of aminoglycoside antibiotics by HPLC/MS

HPLC/MS을 이용한 aminoglycoside 항생제 동시 분석에 관한 연구

  • Roh, Young-Sun (Jeonbuk Livestock Development and Research Institute) ;
  • Baek, Gui-Jung (Jeonbuk Livestock Development and Research Institute) ;
  • Kim, Seung-Yong (Jeonbuk Livestock Development and Research Institute) ;
  • Choi, Eun-Young (Jeonbuk Livestock Development and Research Institute) ;
  • Seo, Heyng-Seok (Jeonbuk Livestock Development and Research Institute) ;
  • Hur, Boo-Hong (Jeonbuk Livestock Development and Research Institute) ;
  • Joung, Dong-Suk (Jeonbuk Livestock Development and Research Institute)
  • 노영선 (전라북도축산진흥연구소) ;
  • 백귀정 (전라북도축산진흥연구소) ;
  • 김승용 (전라북도축산진흥연구소) ;
  • 최은영 (전라북도축산진흥연구소) ;
  • 서형석 (전라북도축산진흥연구소) ;
  • 허부홍 (전라북도축산진흥연구소) ;
  • 정동석 (전라북도축산진흥연구소)
  • Published : 2007.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

A liquid chromatographic method has been developed for the analysis of aminoglycoside antibiotics (AMGs) using Heptafluorobutyric acid (HFBA) as a ion-pairing reagent. AMGs (amikacin, apramycin, dihydrostreptomycin, gentamicin, hygrosin B, kanamycin, neomycin, spectinomycin and tobramycin) were formed by reaction with HFBA as ion-pairing reagent. HFBA was attached to corresponding amino group of AMGs. These AMGs compounds were separated and detected by electrospray ionization mass spectrometry (ESI-MS). The experimental conditions for separation of AMGs were optimized and validated. A simple liquid chromatographic method for the determination of AMGs was demonstrated.

Keywords

References

  1. 정희영. 1985. 항생제의 길잡이. 수문사. 서울:238-265
  2. 홍사덕. 1987. 이우주의 약리학강의. 의학문화사. 서울:547-5512
  3. 이장락. 1987. 수의약리학. 서울대학교 출판부. 서울:392-394
  4. Brown SA, Newkirk DR, Hunter RP, et al. 1990. Extraction methods for quantitation of gentamicin residues from tissues using fluorescence polarization immunoassay. J Assoc Off Anal Chem 73(3):479-483
  5. Kim BH, Lee SC, Lee HJ, et al. 2003. Reverse-phase liquid chromatographic method for the analysis of aminogly‐coside antibiotics using pre-column derivation with phenyisocyanate. Biomed Chromatogr 17(6):396-403 https://doi.org/10.1002/bmc.254
  6. Posyniak A, Zmudzki J, Niedzielska J. 2001. Sample preparation for residue determination of gentamicin and neo‐mycin by liquid chromatography. J Chro‐matogr A 914(1-2):59-66 https://doi.org/10.1016/S0021-9673(01)00607-0
  7. Isoherranen N, Soback S. 2000. Determination of gentamicins C1, C1a and C2 in plasma and urine by HPLC. Clin Chem 46(6 Pt 1):837-842
  8. Shaikh B, Jackson J. 1992. Improved liquid chromatograhic determination of neomycin B in bovine kidney. J AOAC Int 76(3):543-548
  9. Per H, Sonja K. 1982. Improved high-performance liquid chromatogra-phic method for simultaneous determination of neamine, neomycin B and neomycin C in neomycin sulfate. J Chromatogr 235:215-220 https://doi.org/10.1016/S0021-9673(00)95802-3
  10. Claes PJ, Busson R, Vanderhaeghe H. 1984. Determination of the component ratio of commercial gentamicins by high-performance liquid chromatogra‐phy using pre-column derivatization. J Chromatogr 298(3):445-457 https://doi.org/10.1016/S0021-9673(01)92742-6
  11. Maitra SK, Yoshikawa TT, Hansen JL, et al. 1977. Serum gentamicin assay by high-performance liquid chromatogra‐phy. Clin Chem 23(12):2275-2278
  12. Reid JA, MacNeil JD. 1999. Determination of neomycin in animal tissues by liquid chromatography. J AOAC Int 82(1):61-67
  13. Clarot I, Chaimbault P, Hasdenteufel F, et al. 2004. Determination of gentamicin sulfate and related compound by high-performance liquid chromatogra‐phy with evaporative light scattering detection. J Chromatogr A 1031(1-2):281-287 https://doi.org/10.1016/j.chroma.2003.08.108
  14. Megoulas NC, Koupparis MA. 2004. Development and validation of a novel LC/ELSD method for the quantitation of gentamicin sulfate components in pharmaceuticals. J Pharm Biomed Anal 36(1):73-79 https://doi.org/10.1016/j.jpba.2004.05.018
  15. Kubo H, Kobayashi Y, Nishikawa T. 1985. Rapid method for determination of kanamycin and dibekacin in serum by use of high-pressure liquid chromato‐graphy. Antimicrob Agents Chemother 28(4):521-523 https://doi.org/10.1128/AAC.28.4.521
  16. Frank J. Shenck, Patrick SC. 1998. Chromatographic method of analysis of antibiotics in milk. J AOAC Int A 812:99-109
  17. Getek TA, Vestal ML. 1991. Analysis of gentamycin sulfate by high-performance liquid chromatography combine with thermospray mass spectrometry. J Chromatogr 554:191-203 https://doi.org/10.1016/S0021-9673(01)88449-1
  18. United State Department of Agriculture. 2003. Confirmation of Aminoglycosides by HPLC-MS/MS. United State Department of Agriculture Food Safety Inspection Service, Office of Public Health and Science. CLG-AMG 1.00:1-18
  19. Oertel R, Neumeister V, Kirch W. 2004. Hydrophilic interaction chromatography combined with tandem-mass spectro‐metry to determine six AMGs in serum. J Chromatogr A 1058(1-2):197-201 https://doi.org/10.1016/S0021-9673(04)01570-5
  20. 식품의약품안정청. 2005. 식품의 기준 및 규격(식품의약품안정청고시 2005-27호) : 59-69