Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
권호 표지
DOI QR코드

DOI QR Code

In vitro Peroxynitrite Scavenging Activity of Arctic Seaweed Extracts

  • Seo, Young-Wan (Division of Marine Environment and Bioscience Korea Maritime University) ;
  • Lee, Hee-Jung (Busan Regional Food & Drug Administration, Test & Analysis Center) ;
  • Kim, You-Ah (Division of Marine Environment and Bioscience Korea Maritime University) ;
  • Lee, Jung-Im (Division of Marine Environment and Bioscience Korea Maritime University) ;
  • Kim, Ji-Hee (Korea Polar Research Institute, KORDI) ;
  • Chung, Ho-Sung (Korea Polar Research Institute, KORDI) ;
  • Kang, Sung-Ho (Korea Polar Research Institute, KORDI)
  • Published : 2007.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, antioxidant activities of acetone/dichloromethane and methanol extracts of Arctic seaweeds were investigated. The antioxidant properties of both extracts of arctic seaweed were evaluated using two different peroxynitrite tests, including scavenging power on authentic peroxynitrite and inhibitory activities on peroxynitrite generation from 3-morpholinosydnonimine (SIN-1) producing superoxide anion and nitric oxide simultaneously in vitro. At concentration of $10\;{\mu}g/ml$, the acetone/dichloromethane and methanol extracts of Odonthalia dentata exhibited 54.6 and 64.2% inhibition against peroxynitrite generation from SIN-1 while they exhibited 24.3 and 23.0% scavenging activities on authentic peroxynitrite, respectively. On the other hand, the acetone/dichloromethane extract of Polysiphonia stricta exhibited 61.8% inhibition only against the induced peroxynitrite from SIN-1. Furthermore, the crude extracts of Odonthalia dentata and Polysiphonia stricta were fractionated into n-hexane, 85% aq. MeOH, n-BuOH, and $H_2O$ fractions, successively, and only 85% aq. MeOH fraction exhibited the best inhibition.

Keywords

References

  1. Aknin, M., A. Samb, J. Mirailles, V. Costantino, E. Fattorusso, and A. Mangoni. 1992. Polysiphenol, a new brominated 9,10-dihydrophenanthrene from the senegalese red alga polysyphonia ferulacea. Tetrahedron Lett., 33, 555-558 https://doi.org/10.1016/S0040-4039(00)93994-7
  2. Ali, M.S., M. Jahangir, M ., Saleen, M .K. Pervez, S . Hameed, and V.U. Ahmad. 2000. Metabolites of marine algae collected from Karachi-coasts of arabian sea. Nat. Prod. Sci., 6, 61-65
  3. Choi, J.S., H.Y. Chung, S.S. Kang, M.J. Jung, J.W. Kim, J.K. No, and H.A. Jung. 2002. The structure-activity relationship of flavonoids as scavengers of peroxynitrite. Phytochem. Res., 16, 232-235
  4. Chung, H.Y., H.R. Choi, H.J. Park, J.S. Choi, and W.C. Choi. 2001. Peroxynitrite scavenging and cytoprotective activity of 2,3,6-tribromo-4,5-dihydroxybenzyl methyl ether from the marine alga Symphyocladia latiuscula. J. Agr. Food Chem., 49, 3614-3621 https://doi.org/10.1021/jf0101206
  5. Fang, Y.Z., S. Yang, and G. Wu. 2002. Free radicals, antioxidants and Nutrition. Nutrition, 18, 872-879 https://doi.org/10.1016/S0899-9007(02)00916-4
  6. Flodin, C. and F.B. Whitfield. 2000. Brominated anisoles and cresols in the red alga Polysiphonia sphaerocarpa. Phytochemistry, 53, 77-80 https://doi.org/10.1016/S0031-9422(99)00429-X
  7. Grace, S.C., M.G. Salgo, and W.A. Pryor. 1998. Scavenging of peroxynitrite by a phenolic/peroxidase system prevents oxidative damage to DNA. FEBS Lett., 426, 24-28 https://doi.org/10.1016/S0014-5793(98)00298-1
  8. Jung, H.A., H.J. Lee, Y.A. Kim, K.E. Park, J.W. Ahn, B.J. Lee, S.G. Moon, and Y. Seo. 2004. Antioxidant activity of Artemisia capillaris Thunberg. Food Sci. Biotechnol., 13, 328-331
  9. Jung, H.A., S.K. Hyun, H.R. Kim, and J.S. Choi. 2006. Angiotensin-converting enzyme I inhibitory activity of phlorotannins from Ecklonia stolonifera. Fish. Sci., 72, 1292-1299 https://doi.org/10.1111/j.1444-2906.2006.01288.x
  10. Kooy, N.W., J.A., Royall, H. Ischiropoulos, and J.S. Beckman. 1994. Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Radic. Biol. Med., 16, 149-156 https://doi.org/10.1016/0891-5849(94)90138-4
  11. Kurata, K. and K. Taniguchii. 1997. Feeding-deterrent bromophenols from Odonthalia corymbifera. Phytochemistry, 45, 485-487 https://doi.org/10.1016/S0031-9422(97)00014-9
  12. Lee, H.J., Y.A. Kim, J.W. Ahn, B.J. Lee, S.G. Moon, and Y. Seo. 2004a. Screening of peroxynitrite and DPPH radical scavenging activities from salt marsh plants. Kor. J. Biotechnol. Bioeng., 19, 57-61
  13. Lee, H .J., K .E. Park, J.S. Y oo, J.W. A h n, B .J. Lee, a nd Y . Seo. 2004b. Studies on screening of seaweed extracts for peroxynitrite and DPPH radical scavenging activities. Ocean and Polar Res., 26, 59-64 https://doi.org/10.4217/OPR.2004.26.1.059
  14. Lee, H .-J., K.E. P ark, Y .A. Kim, J .W. Ah n, J .S. Yoo, a nd B.J. Lee. 2004. Peroxynitrite-scavenging constituents from the brown alga Sargassum thunbergii. Biotechnol. Bioprocess Eng., 9, 212-216 https://doi.org/10.1007/BF02942295
  15. Lee, H.J. and Y. Seo. 2006. Antioxidant properties of Erigeron annuus extract and its three phenolic constituents. Biotechnol. Bioeng., 11, 13-18 https://doi.org/10.1007/BF02931862
  16. Patel, R.P., J. McAndrew, H. Sellak, C. Roger White, H. Jo, B.A. Freeman, and V.M. Darley-Usmar. 1999. Biological aspects of reactive nitrogen species. Biochim. Biophys. Acta, 1411, 385-400 https://doi.org/10.1016/S0005-2728(99)00028-6
  17. Perdersen, M. 1978. Bromochlorophenols and a brominated diphenylmethane in red algae. Phytochemistry, 17, 291-293 https://doi.org/10.1016/S0031-9422(00)94167-0
  18. Reiter, R.J., D.X. Tan, and S. Burkhardt. 2002. Reactive oxygen and nitrogen species and cellular and organismal decline: amelioration with melatonin. Mech. Aging Dev., 123, 1007-1019 https://doi.org/10.1016/S0047-6374(01)00384-0
  19. Rice-Evans, C.A., N.J. Miller, and G. Paganga. 1996. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biol. Med., 20, 933-956 https://doi.org/10.1016/0891-5849(95)02227-9
  20. Seo, Y., H.J. Lee, K.E. Park, Y.A. Kim, J.W. Ahn, J.S. Yoo, and B.J. Lee. 2004. Peroxynitrite scavenging constituents from the brown Alga Sargassum thunbergii. Biotechnol. Bioeng., 9, 212-216 https://doi.org/10.1007/BF02942295
  21. Virag, L., E. Szabo, P. Gergely, and C. Szabo. 2003. Peroxynitrite- induced cytotoxicity: mechanism and opportunities for intervention. Toxicol. Lett., 140/141, 113-124 https://doi.org/10.1016/S0378-4274(02)00508-8
  22. Yokozawa, T., C.P. Chen, E. Dong, T. Tanaka, G.I. Nonaka, and I. Nishioka. 1998. Study on the inhibitory effect of tannins and flavonoids against the 1,1-diphenyl-2-picrylhydrazyl radicals. Biochem. Pharmacol., 56, 213-222 https://doi.org/10.1016/S0006-2952(98)00128-2
  23. Zou, Y., A.R. Kim, J.E. Kim, J.S. Choi, and H.Y. Chung. 2002. Peroxynitrite scavenging activity of sinapic acid (3,5-dimethoxyl-4-hydroxycinnamic acid) isolated from Brassica juncea. J. Agric. Food Chem., 50, 5884-5890 https://doi.org/10.1021/jf020496z

Cited by

  1. Antioxidant Capacity of Crude Extract and Its Solvent Fractions of Arctic Terrestrial Plant Ranunculus heperporeus vol.38, pp.3, 2016, https://doi.org/10.4217/OPR.2016.38.3.185
  2. Isolation and Antioxidant Activity of Methyl Aconitates from Arctic Red Alga Polysiphonia stricta vol.36, pp.3, 2014, https://doi.org/10.4217/OPR.2014.36.3.247