Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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HPLC Analysis of Saponins in Platycodi Radix

HPLC를 이용한 길경(桔梗) 사포닌 분석법(分析法)

  • Kim, Hyun-Ki (Korea Research Institute of Chemical Technology) ;
  • Choi, Jae-Seok (Korea Research Institute of Chemical Technology) ;
  • Yoo, Dae-Seok (Korea Research Institute of Chemical Technology) ;
  • Choi, Yeon-Hee (Korea Research Institute of Chemical Technology) ;
  • Yon, Gyu-Hwan (Korea Research Institute of Chemical Technology) ;
  • Hong, Kyung-Sik (Korea Research Institute of Chemical Technology) ;
  • Lee, Byung-Hoi (Korea Research Institute of Chemical Technology) ;
  • Kim, Hye-Jin (Korea Research Institute of Chemical Technology) ;
  • Kim, Eun-Ju (JangSaeng Doraji Research Institute of Biotechnology, JangSaeng Doraji Co. LTD) ;
  • Park, Byoung-Keun (JangSaeng Doraji Research Institute of Biotechnology, JangSaeng Doraji Co. LTD) ;
  • Jeong, Young-Chul (Division of Food science, Jinju International University) ;
  • Kim, Young-Sup (Korea Research Institute of Chemical Technology) ;
  • Ryu, Shi-Yong (Korea Research Institute of Chemical Technology)
  • Published : 2007.06.30
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Abstract

A rapid and practical HPLC assay was developed for quantitative analysis of saponins in Platycodi Radix. Seven saponin components in Platycodi Radix, i.e., deapioplatycoside E, platycoside E, deapioplatycodin D$_3$, platycodin D$_3$, polygalacin D$_2$, platycodin D$_2$ and platycodin D were successfully resolved on C18 column and detected by ELSD. Standard curves were linear over the concentration range 1 ${\sim}$2,000 ${\mu}$g/ml (r$^2$>0.992). Intra- and inter-day coefficients for variation of seven saponin components were < 10% and limit of quantification of them were around 0.7${\sim}$1.5 ${\mu}$g/ml, respectively. Using this method, contents of seven saponins in various plant materials under different cultivating conditions were estimated.

Keywords

References

  1. Kim, Y.P., Lee, E. B., Kim, S. Y., Li, D., Ban, H. S., Lim, S. S., Shin, K. H. and Ohuchi, K. (2001) Inhibition of prostaglandin E2 production by platycodin D isolated from the root of Platycodon grandiflorum. Planta Med. 67: 362-364 https://doi.org/10.1055/s-2001-14317
  2. Shin, C. Y., Lee, W. J., Lee, E. B., Choi, E. Y. and Ko, K. H. (2002) Platycodin D and D3 increase airway mucin release in vivo and in vitro in rats and hamsters. Planta Med. 68: 221-225 https://doi.org/10.1055/s-2002-23130
  3. Lee, K.J. and Jeong, H.G. (2002) Protective effect of Platycodi Radix on carbon tetrachloride-induced hepatotoxicity. Food Chem. Toxicol., 40: 517-525 https://doi.org/10.1016/S0278-6915(01)00104-1
  4. Lee, K.J., You, H.J., Park, S.J., Kim, Y.S., Chung, Y.C., Jeong, T.C. and Jeong, H.G.(2001) Hepatoprotective effects of Platycodon grandiflorum on acetaminophen -induced liver damage in mice. Cancer Lett., 174: 73-81 https://doi.org/10.1016/S0304-3835(01)00678-4
  5. Choi, C.Y., Kim, J.Y., Kim, Y.S., Chung, Y.C., Seo, J.K. and Jeong, H.G. (2001) Aqueous extract isolated from Platycodon grandiflorumelicits the release of nitric oxide and tumor necrosis factor-a from murine macrophages. International Immunopharmacology, 1: 1141-1151 https://doi.org/10.1016/S1567-5769(01)00047-9
  6. Choi, C.Y., Kim, J.Y., Kim, Y.S., Chung, Y.C., Hahm, K.S. and Jeong, H.G. (2001) Augmentation of macrophage functions by an aqueous extract isolated from Platycodon grandiflorum. Cancer Lett., 166: 17-25 https://doi.org/10.1016/S0304-3835(01)00440-2
  7. Saeki T, Koide K and Nikaide T (1999) A comparative study on commercial, botanical gardens and wild samples of the roots of Platycodon grandiflorum by HPLC analysis. Planta Med. 65: 428-431 https://doi.org/10.1055/s-1999-14021
  8. Kim, G.S., Kim, H.T., Seong. J.D., Park, H.S., Kim, S.D.(2002) Quantitative analysis of platycodin d from Platycodon grandiflorum by HPLC-ELSD. Korean J. Medicinal Crop Sci. 10: 200-205
  9. Kim, Y.S., Kim, J.S., Choi, S.U., Kim, J.S., Lee, H.S., Roh, S.H., Jeong, Y.C., Kim, Y.K. and Ryu, S.Y.(2005) Isolation of a new saponin and cytotoxic effect of saponins from the root of Platycodon grandiflorum on human tumor cell lines. Planta Med. 71: 566-568 https://doi.org/10.1055/s-2005-864161