Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Enhancement of Immune Activities of Kadsura Japonica Dunal. through Conventional Fermentation Process

오미자의 전통발효에 의한 면역활성 증진

  • Kim, Cheol-Hee (School of Biotechnology & Bioengineering, Kangwon Natl. Univ., Research Institute of Bioscience and Biotechnology, Kangwon National Uniersity) ;
  • Kwon, Min-Chul (School of Biotechnology & Bioengineering, Kangwon Natl. Univ.) ;
  • Kim, Hyo-Sung (School of Biotechnology & Bioengineering, Kangwon Natl. Univ.) ;
  • Ahn, Ju-Hee (School of Biotechnology & Bioengineering, Kangwon Natl. Univ.) ;
  • Chio, Geun-Pyo (Department of Food and Life Science, Gangwon Provincial Univ.) ;
  • Choi, Young-Beom (Jeong Woo Foods Co.) ;
  • Ko, Jung-Rim (Jeong Woo Foods Co.) ;
  • Lee, Hyeon-Young (School of Biotechnology & Bioengineering, Kangwon Natl. Univ.)
  • 김철희 (강원대학교 BT특성화학부대학) ;
  • 권민철 (강원대학교 BT특성화학부대학) ;
  • 김효성 (강원대학교 BT특성화학부대학) ;
  • 안주희 (강원대학교 BT특성화학부대학) ;
  • 최근표 (강원도립대학 식품생명과학과) ;
  • 최영범 (정우식품) ;
  • 고정림 (정우식품) ;
  • 이현용 (강원대학교 BT특성화학부대학, 강원대학교 생명공학연구소)
  • Published : 2007.06.30
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Abstract

Immune activities of two different extracts of Kadsura japonica Dunal. by typical extraction processes using water and ethanol at 60 $^{\circ}$C and 100 $^{\circ}$C were compared to them by ultrasofication system and through traditional fermemtation process. The fermented broth Kadsura japonica Dunal. definitely improved the growth of human B and T cell up to 30% and 22%, respectively, compared to the control. The secretion of TNF-${\alpha}$ and IL-60 was also enhanced by the addition of the fermented broth, up to 35%. NK cell activation was significantly improved up to 1.4 times higher than the case of adding other extracts. It was also found that this broth could yield higher nitric oxide production from macrophage than Lipopolysaccharides (LPS). It can be concluded that Kadsura japonica has immune activities and, in general, the culture broth from a conventional fermentation has higher immune activities, possibly by yielding immuno-modulatory compounds, not existed in typcial extraction systems as the result of HPLC analysis.

여러 가지 추출조건을 통해 얻은 오미자 추출물과 전통발효공정을 통해 얻은 오미자 발효액의 면역증진 활성을 비교하였다. 오미자 발효액이 B cell과 T cell에서 시료를 첨가하지 않은 대조군과 비교하여 각각 30%와 22%이상의 수치를 나타내며 가장 좋은 활성을 나타내었다. B, T cell의 cytokine 분비량 측정을 통한 실험에서도 오미자의 전통발효액이 생육증진과 유의적으로 가장 많은 분비량을 나타내었다. 대식세포를 이용한 NO$^-$ 생성능 측정에서 오미자 발효액 및 추출물의 활성이 LPS와 비교해 비슷하거나 높은 것을 확인하였다. 또한 시료를 LPS와 같이 첨가하였을 때 시료만을 첨가했을 때보다 높은 활성을 나타내어 농도에 따른 유의적 활성 증가가 이루어질 것으로 사료된다. NK cell의 활성측정에서 모든 시료 조건에서 6일 동안 생육이 증가하였으며 오미자 발효액을 첨가한 것이 아무것도 첨가하지 않은 대조군과 비교하여 약 1.4배의 생육도 증진을 나타내어 1.2배의 생육도 증진을 보인 100$^{\circ}$C에서 초음파를 병행한 물 추출물보다 높은 활성을 나타내었다. HPLC를 이용한 추출물들의 성분 분석에서 전통발효 공정을 통해 추출공정에서 확인할 수 없었던 특정 성분의 생성 및 기존 성분의 용출 증진이 이루어지는 것을 확인하였고, 이에 따라 면역활성의 증진이 이루어지는 것으로 추정할 수 있었다. 이상의 결과에서 오미자는 면역활성을 가지는 성분을 함유하고 있으며 전통발효 공정을 통해 생성되는 대사산물이 면역실험에서 유효활성 증진을 나타내는 것을 확인하였다. 이는 전통발효 공정을 통해 생성된 성분의 활성으로 세포독성이 감소하고 세포활성이 증가하기 때문인 것으로 생각된다. 이상의결과는 기존에 면역활성 증진 효과를 나타내는 초음파병행 공정 보다 높은 증진 효과로 전통발효 공정 및 활성성분에 대한 다각적인 연구가 필요할 것으로 사료된다.

Keywords

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