Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Comparison of Immune modulatary and Anticancer Activities according to the Parts of the Styrax japonica Sieb. et Zucc.

때죽나무의 부위별 면역 및 항암활성 비교

  • Kwon, Oh-Woung (Korea Forest Research Institute) ;
  • Kim, Cheol-Hee (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Kim, Hyo-Sung (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Kwon, Min-Chul (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Ahn, Ju-Hee (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Lee, Hak-Ju (Korea Forest Research Institute) ;
  • Kang, Ha-Young (Korea Forest Research Institute) ;
  • Lee, Hyeon-Yong (College of Bioscience and Biotechnology, Kangwon National University, Research Institute of Bioscience and Biotechnology, Kangwon National Uniersity)
  • 권오웅 (국립산림과학원) ;
  • 김철희 (강원대학교 BT특성화학부대학) ;
  • 김효성 (강원대학교 BT특성화학부대학) ;
  • 권민철 (강원대학교 BT특성화학부대학) ;
  • 안주희 (강원대학교 BT특성화학부대학) ;
  • 이학주 (국립산림과학원) ;
  • 강하영 (국립산림과학원) ;
  • 이현용 (강원대학교 BT특성화학부대학, 강원대학교 생명공학연구소)
  • Published : 2007.06.30
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Abstract

This study was performed to anticancer activities and immune modulatary activities according to the parts of the S. japonica Sieb. et Zucc. The cytotoxicity on human kidney cell (HEK 293) was showed below 27.4% in adding the methanol extracts. The anticancer activity were increased in over 60% by barks extracts in AGS and MCF-7 cells. The immune cell growth using human immune B and T cells was improved by the barks extracts of S. japonica Sieb. et Zucc. in adding 1.0mg/ml concentration. The secretion of the IL-6 and TNF-${\alpha}$ from human immune B and T cells was showed secretion for the amount of cytokines by bark extracts of S. japonica Sieb. et Zucc. NK cell growth was increased against control all of the extracts of S. japonica Sieb. et Zucc. Densitometric analysis of Bcl-2 revealed that possible to decrease potentialities of taking cancer in adding of extracts from S. japonica Sieb. et Zucc. From the results, the roots and barks extracts of S. japonica Sieb. et Zucc. were showed useful biological activities.

B cell과 T cell을 이용한 면역세포의 생육 촉진 실험에서, B${\cdot}$T cell은 배양 6일째 수피 추출물이 각각 $4.2{\times}10^4$cells/ml, $5.0{\times}10^4$cells/ml로 가장 높은 세포농도를 나타냈다. 면역세포를 이용한 cytokine의 분비량 측정실험에서는 수피추출물이 배양 시간에 따른 cytokine의 분비가 가장 높게 나타나는 것을 확인할 수 있었다. 각 세포의 TNF-${\alpha}$의 분비량은 6일째 최고의 분비량을 나타내었고, 수피 추출물의 경우 대조군에 비해 7-8배 가량 더 높은 분비량을 나타낸 것을 확인하였다. IL-6의 경우 6일째 최고의 분비량을 나타내었고, 수피 추출물에서 가장 높은 분비량을 나타내었다. 또한 때죽나무 추출물을 첨가한 배지에 의한 NK cell의 면역활성을 측정하였으며, 모든 추출물에서 배양시간에 따라 유의적으로 증가하는 것을 확인하였다. 그 중 때죽나무 수피 추출물에서 B cell의 경우 $14.1{\times}10^4$cells/ml, T cell의 경우 $15.3{\times}10^4$cells/ml 으로 가장 높은 생육 활성을 나타내었다. 인간 정상 신장세포인 HEK293을 이용한 세포 독성을 살펴본 결과, 각 추출물은 1.0mg/ml의 농도에서 최고 27.4%의 세포독성을 나타내었다. 또한 항암활성 효과를 살펴보기 위하여 2가지의 암세포를 이용하였는데, AGS와 MCF-7에서 뿌리추출물의 경우 1.0mg/ml의 농도에서 65정도의 높은 억제활성을 나타내었고, 또한 암세포의 생육활성에 대한 정상 세포의 세포독성의 비로 나타낸 선택적 사멸도는 고농도에서는 모두 1.5이상으로 나타나 모두 암세포에 대한 선택성이 있는 것으로 나타났다. Bcl-2 단백질 정량을 통한 항암활성 실험에서는, IOD 값이 수피, 목부, 잎 순으로 각각 72, 186, 200의 결과를 나타내었다. 본 실험 결과를 통하여 때죽나무의 기능성 소재로서의 가능성을 엿볼 수 있었고, 유용성분 분리와 생리활성에 대한 연구가 보다 다각적으로 이루어져야 할 것으로 생각된다.

Keywords

References

  1. Andersen MH, Svane IM, Kvistborg P, Nielsen OJ, Balslev, Reker S, Becker JC, Straten PT (2005) Immunogenicity of Bcl-2 in patients with cancer. Immunobiology 105(2):728-734
  2. Campos L, Rouault JP, Sabido O, Oriol P, Roubi N, Vasselon C, Archimbaud E, Magaud JP, Guyotat D (1993) High expression bcl-2 protein in acute myeloid leukemia cells is associated with poor response to chemotherapy. Blood 18:3091-3096
  3. Castle VP, Heidelberger KP, Bromberg J, Ou X, Dole M, Nunez G (1993) Expression of the apoptosis-suppressing protein bcl-2, In neuroblastoma is associated with unfavorable histology and N-myc amplication. The American journal of pathology 143:1543-1550
  4. Clark WH (1991) Tumor progression and the nature of cancer. British journal of cancer 64:631-644 https://doi.org/10.1038/bjc.1991.375
  5. Colombel M, Symmans F, Gil S, O'Toole KM, Chopin D, Benson M, Olsson CA, Korsmeyer S, Buttyan R (1993) Detection of the apoptosis-suppressing oncoprotein bcl-2 in hormone refractory human prostate cancers. The American journal of pathology 143:390-400
  6. Doll R, Peto R (1981) The Causes of Cancer, Quantitative Estimates of Avoidable Risks of Cancer in the United States today. Journal of the National Cancer Institute 66(6):1192
  7. Han BH, Park MH, Cho JY, Park JS, Yoo ES, Baik KU (1998) Effect of Ginsenosides from Panax ginseng on TNF-a production and T cell proliferation. Pharmaceutical Society of Korea 42(3):296-301
  8. Hirano T, Goto M, Oka K (1994) Natural flavonoids and lignans are potent cytosaticc agents against human leukemic HL-60 cells. Life Sciences 55:1061-1069 https://doi.org/10.1016/0024-3205(94)00641-5
  9. Hockenbery D, Nunez G, Milliman C, Schreiber RD, Korsmeyer SJ (1990) Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 348:334-336 https://doi.org/10.1038/348334a0
  10. Kerr JF, Willie AH, Currie AR (1972) Apoptosis; a basic biologic phenomenon with wide-ranging implications in tissue kinetics. British journal of cancer 26:239-257 https://doi.org/10.1038/bjc.1972.33
  11. Korsmeyer (1992) Bcl-2 indicates a new category of oncogenes; Regulations of cell death. Blood 80:879
  12. Lee DH, Kim JH, Huh JR, Kim DJ, Kim YM, Kim YT, Nam JH, Mok JE (1998) Expression and Prognostic Significance of bcl-2 in Epithelial Ovarian Tumors. Korean Journal of Obstetrics and Gynecology 41(5):1304-1314
  13. Lee JM, Cho HI, Kang JS (1999) The Studies for Usefilness of Overexpression of bcl-2, p53 Gene and Apoptosis as a Response Predictor to neoadjuvant Chemotherapy in Cervical Cancer. Korean Journal of Obstetrics and Gynecology 42(5):1096-1101
  14. Lee SH, Lee HS, Park YS, Hwang B, Kim JH, Lee HY (2002) Screening of Immune Activation Activation Activities in the Leaves of Dendropanax morbifera Lev. Korean Journal of Medicinal Crop Science 10:109-115
  15. Lee YN (1996) Flora of Korea. Kyohak Pub. Co., Seoul. p. 606
  16. Leek RD, Kaklamanis L, Pezzela F, Garter KC, Harris AL (1994) Bcl-2 in normal human breast and carcinoma, association with estrogen receptor-positive, epidermal growth factor receptornegative tumors and in situ cancer. British joumal of cancer 69:135-139 https://doi.org/10.1038/bjc.1994.22
  17. Limdbolum CK (2002) IL-2 receptor signaling through the Shb adapter protein in T and NK cells. Biochemical and Biophysical Research Communications 296:929-936 https://doi.org/10.1016/S0006-291X(02)02016-8
  18. McDonnell TJ, Deane N, Platt FM, Nunez G, Jaeger U, McKearn JP, Korsmeyer SJ (1989) Bcl-2 immunoglobulin transgenic mice demonstrate extended B cell survival and follicular Iymphoproliferation. Cell 57:79-88 https://doi.org/10.1016/0092-8674(89)90174-8
  19. Pezzelia F, Turley H, Kuzu I et al. (1994) Bcl-2 protein in nonsmall-cell lung carcinoma The New England journal of medicine 329:690-694 https://doi.org/10.1056/NEJM199309023291003
  20. Silvestrini R, Veneroni S, Daidone MG, Benini E, Boracchi P, Mezzetti M, Di Fronzo G, Rilke F, Veronesi U (1994) The bcl-2 protein; a prognostic indicator strongly related to p53 protein in lymph node-negative breast cancer patients. Journal of the National Cancer Institute 86:49-504 https://doi.org/10.1093/jnci/86.1.49
  21. Takanashi M, Tajizawa Y. (1998) New benzofurans related to egonol from immature seeds of Styrax obassia, Phytochemistry 27:1224-1226 https://doi.org/10.1016/0031-9422(88)80314-5
  22. Vaux DL, Cory S, Adams JM (1988) Bcl-2 gene promotes hematopoietic cell survival and cooperates with c-myc to immortalizepre-B cells. Nature 335:440-442 https://doi.org/10.1038/335440a0
  23. Yueran Z, Rui S, You L, Chunyi G, Zhigang T (2003) Expression of leptin recrptors and response to leptin stimulation of human natural killer cell lines. Biochemical and Biophysical Research Communications 300:247-252 https://doi.org/10.1016/S0006-291X(02)02838-3
  24. Yunis JJ, Mayor MG, Arnesen MA, Aeppli DP, Oken MM, Frizzera G (1989) Bcl-2 and other genomic alternations in the prognosis of large-cell lymphoma. The New England journal of medicine 320:1047-1054 https://doi.org/10.1056/NEJM198904203201605
  25. 김갑태 (1989) 종자의 전처리가 몇 수종의 포장 발아율에 미치는 영향, 한국임학회지, 78(1):26-29
  26. 오오진 (1991) 쇠비름이 세포성 및 체액성 면역반응계에 끼치는 영향. 숙명여자대학교 대학원 석사학위논문