Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Effects of BSA, PVA, Gonadotropins and Follicle Shell on In Vitro Maturation and In Vitro Fertilization of Porcine Oocytes

  • Cong, Pei-Qing (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Song, Eun-Sook (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Kim, Eui-Sook (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Li, Zhao-Hua (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Zhang, Yong-Hua (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Yi, Young-Joo (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Park, Chang-Sik (Research Center for Transgenic Cloned Pigs, Chungnam National University)
  • Published : 2007.06.30
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Abstract

This study was designed to evaluate effects of BSA, PVA, gonadotropins and follicle shell during IVM of porcine oocytes and subsequent development to the blastocyst stage after IVF. Cumulus oocyte complexes (COCs) were cultured in TCM-199 media containing 4 mg/ml BSA and 1 mg/ml PVA during IVM for 44 hr. To compare the effect of gonadotropins on oocyte maturation, COCs were cultured with FSH+LH, FSH, LH and FSH-LH-free media during IVM. respectively. Also, different number of follicle shells (0, 2, 4 and 6) was used to examine whether the presence of follicle shell in culture medium affects oocyte maturation. The percentages of fertilization and blastocyst formation, respectively, were higher in the medium containing the PVA (49.0 and 17.9%) than those containing the BSA (40.0 and 12.2%). Significantly higher rates of Mil oocytes were in the presence of FSH+LH and FSH (88.6 and 85.1 %) compared to other treatments (64.0 and 53.4% at LH and FSH-LH-free media). Co-culture with inverted follicle shells in 2 ml maturation medium enhanced the developmental competence of porcine oocytes. In conclusion, PVA could be used as a macromolecules instead of BSA, and FSH and follicle shell played important roles in maturation of porcine oocytes.

Keywords

References

  1. Abeydeera LR, Day BN (1997): Fertilization and subsequent development in vitro of pig oocytes inseminated in a modified tris-buffered medium with frozen-thawed ejaculated spermatozoa. Biol Reprod 57: 729-734 https://doi.org/10.1095/biolreprod57.4.729
  2. Abeydeera LR, Wang WH, Cantley TC, Rieke A, Day BN (1998a): Coculture with follicular shell pieces can enhance the developmental competence of pig oocytes after in vitro fertilization: relevance to intracellular glutathione. Biol Reprod 58:213-218 https://doi.org/10.1095/biolreprod58.1.213
  3. Abeydeera LR, Wang WH, Prather RS, Day BN (1998b): Maturation in vitro of pig oocytes in protein-free culture media: fertilization and subsequent embryo development in vitro. Biol Reprod 58:1316-1320 https://doi.org/10.1095/biolreprod58.5.1316
  4. Abeydeera LR (2002): In vitro production of embryos in swine. Theriogenology 57:257-273 https://doi.org/10.1016/S0093-691X(01)00670-7
  5. Bavister BD (1981): Analysis of culture media for in vitro fertilization and criteria of success. In: Mastroianni L, Biggera JD (eds.). Fertilization and Embryonic Development In Vitro. Plenum Press, New York, USA. pp 41-60
  6. Brad AM, Bormann CL, Swain JE, Durkin RE, Johnson AE, Clifford AL, Krisher RL (2003): Glutathione and adenosine triphosphate content of in vivo and in vitro matured porcine oocytes. Mol Reprod Dev 64:492-498 https://doi.org/10.1002/mrd.10254
  7. Coy P, Romar R, Ruiz S, Canovas S, Gadea J, Garcia VF, Matas C (2005): Birth of piglets after transferring of in vitro-produced embryos pre-matured with R-roscovitine. Reproduction 129:747-755 https://doi.org/10.1530/rep.1.00691
  8. De Matos DG, Furnus CC, Moses DF, Baldassarre H (1995): Effect of cysteamine on glutathione level and developmental capacity of bovine oocyte matured in vitro. Mol Reprod Dev 43:432-436
  9. Ding J, Foxcroft GR (1992): Follicular heterogeneity and oocyte maturation in vitro in pigs. Biol Reprod 47:648-655 https://doi.org/10.1095/biolreprod47.4.648
  10. Ding J, Foxcroft GR (1994): Epidermal growth factor enhances oocyte maturation in pigs. Mol Reprod Dev 39:30-40 https://doi.org/10.1002/mrd.1080390106
  11. Funahashi H, Cantley TC, Stumpf TT, Terlouw SL, Day BN (1994a): In vitro development of in vitromatured porcine oocytes following chemical activation or in vitro fertilization. Biol Reprod 50:1072-1077 https://doi.org/10.1095/biolreprod50.5.1072
  12. Funahashi H, Stumpf TT, Terlouw SL, Cantley T, Rieke A, Day BN (1994b): Developmental ability of pig oocytes matured and fertilized in vitro. Theriogenology 41:1425-1433 https://doi.org/10.1016/0093-691X(94)90193-M
  13. Funahashi H, Kim NH, Stumpf TT, Cantley TC, Day BN (1996): Presence of organic osmolytes in maturation medium enhances cytoplasmic maturation of porcine oocytes. Biol Reprod 54:1412-1419 https://doi.org/10.1095/biolreprod54.6.1412
  14. Funahashi H, Cantley TC, Day BN (1997): Synchronization of meiosis in porcine oocytes by exposure to dibutyryl cyclic adenosine monophosphate improves developmental competence following in vitro fertilization. Biol Reprod 57:49-53 https://doi.org/10.1095/biolreprod57.1.49
  15. Grupen CG, Nagashima H, Nottle MB (1995): Cysteamine enhances in vitro development of porcine oocytes matured and fertilized in vitro. Biol Reprod 53:173-178 https://doi.org/10.1095/biolreprod53.1.173
  16. Grupen CG, Nagashima H, Nottle MB (1997): Role of epidermal growth factor and insulin-like growth factor-I on porcine oocyte maturation and embryonic development in vitro. Reprod Fertil Dev 9:571-575 https://doi.org/10.1071/R96115
  17. Hagen DR, Prather RS, Sims MM, First NL (1991): Development of one-cell porcine embryos to the blastocyst stage in simple media. J Anim Sci 69: 1147-1150 https://doi.org/10.2527/1991.6931147x
  18. Hoshino Y, Uchida M, Shimatsu Y, Miyake M, Nagao Y, Minami N, Yamada M, Mai H (2005): evelopmental competence of somatic cell nuclear transfer embryos reconstructed from oocytes matured in vitro with follicle shells in miniature pig. Cloning Stem Cells 7:17-26 https://doi.org/10.1089/clo.2005.7.17
  19. Iwasaki T, Kimura E, Totsukawa K (1999): Studies on a chemically defined medium for in vitro culture of in vitro matured and fertilized porcine oocytes. Theriogenology 51:709-720 https://doi.org/10.1016/S0093-691X(99)00020-5
  20. Kano K, Miyano T, Kato S (1998): Effects of glycosaminoglycans on the development of in vitro-matured and - fertilized porcine oocytes to the blastocyst stage in vitro. Biol Reprod 58:1226-1232 https://doi.org/10.1095/biolreprod58.5.1226
  21. Liu L, Dai Y, Moor RM (1997): Role of secreted proteins and gonadotropins in promoting full maturation of porcine oocytes in vitro. Mol Reprod Dev 47:191-199 https://doi.org/10.1002/(SICI)1098-2795(199706)47:2<191::AID-MRD10>3.0.CO;2-3
  22. Mattioli M, Galeati G, Seren E (1988): Effect of follicle somatic cells during pig oocyte maturation on egg penetrability and male pronuclear formation. Gamete Res 20:177-183 https://doi.org/10.1002/mrd.1120200208
  23. Mattioli M, Bacci ML, Galeati G, Seren E (1989): Developmental competence of pig oocytes matured and fertilized in vitro. Theriogenology 31:1201-1207 https://doi.org/10.1016/0093-691X(89)90089-7
  24. Mattioli M, Bacci ML, Galeati G, Seren E (1991): Effects of LH and FSH on the maturation of pig oocytes in vitro. Theriogenology 36:95-105 https://doi.org/10.1016/0093-691X(91)90438-J
  25. Miyano T, Hiro-oka RE, Kano K, Miyake M, Kusunoki H, Kato S (1994): Effects of hyaluronic acid on the development of 1- and 2-cell porcine embryos to the blastocyst stage in vitro. Theriogenology 41:1299-1305 https://doi.org/10.1016/0093-691X(94)90488-5
  26. Naito K, Fukuda Y, Toyoda Y (1988): Effect of procine follicular fluid on male pronucleus formation in porcine oocytes matured in vitro. Gamete Res 21: 289-295 https://doi.org/10.1002/mrd.1120210310
  27. Nasr-Esfahani MH, Johnson MH (1992): Quantitative analysis of cellular glutathione in early preimplantation mouse embryos developing in vivo and in vitro. Hum Reprod 7:1281-1290 https://doi.org/10.1093/oxfordjournals.humrep.a137843
  28. Park KW, Lai L, Cheong HT, Cabot R, Sun QY, Wu GM, Rucker EB, Durtschi D, Bonk A, Samuel M, Rieke A, Day BN, Murphy CN, Carter DB, Prather RS (2002): Mosaic gene expression in nuclear transfer-derived embryos and the production of cloned transgenic pigs from ear-derived fibroblasts. Biol Reprod 66:1001-1005 https://doi.org/10.1095/biolreprod66.4.1001
  29. Park CS, Kim MY, Yi YJ, Chang YJ, Lee SH, Lee JJ, Kim MC, Jin DI (2004): Liquid boar sperm quality during storage and in vitro fertilization and culture of pig oocytes. Asian-Aust J Anim Sci 17:1369-1373 https://doi.org/10.5713/ajas.2004.1369
  30. Shimada M, Terada T (2002): FSH and LH induce progesterone production and progesterone receptor synthesis in cumulus cells: a requirement for meiotic resumption in porcine oocytes. Mol Hum Reprod 8:612-618 https://doi.org/10.1093/molehr/8.7.612
  31. Slott VL, Hales BE (1987): Effect of glutathione depletion by buthionine sulfoximineon rat embryonic development in vitro. Biochem Pharmacol 36: 683-688 https://doi.org/10.1016/0006-2952(87)90719-2
  32. Tatemoto H, Muto N, Sunagawa I, Shinjo A, Nakada T (2004): Protection of porcine oocytes against cell damage caused by oxidative stress during in vitro maturation: Role of superoxide dismutase activity in porcine follicular fluid. Biol Reprod 71:1150-1157 https://doi.org/10.1095/biolreprod.104.029264
  33. Wang WH, Nika K, Okuda K (1991): In vitro penetration of pig oocyts matured in culture by frozen-thawed ejaculated spermtozoa. J Reprod Fertil 93:491-496 https://doi.org/10.1530/jrf.0.0930491
  34. Wang WH, Niwa K (1995): Synergetic effects of epidermal growth factor and gonadotropins on the maturation of pig oocytes in a serum-free medium. Zygote 3:345-350 https://doi.org/10.1017/S096719940000277X
  35. Yamashita Y, Shimada M, Okazaki T, Maeda T, Terada T (2003): Production of progesterone from de novo-synthesized cholesterol in cumulus cells and its physiological role during meiotic resumption of porcine oocytes. Biol Reprod 68:1193-1198 https://doi.org/10.1095/biolreprod.102.010934
  36. Yi YJ, Kim MY, Lee SH, Min TS, Jin DI, Park CS (2003): Effect of cysteamine on in vitro maturation, fertilization and culture of porcine oocytes. Korean J Anim Reprod 27(4):275-280
  37. Yoshida M, Ishigaki K, Pursel VG (1992a): Effects of maturation media on male pronucleus formation in pig oocytes matured in vitro. Mol Reprod Dev 31:68-71 https://doi.org/10.1002/mrd.1080310112
  38. Yoshida M, Ishizaki Y, Kawagishi H, bamba K, Kojima Y (1992b): Effects of pig follicular fluid on maturation and after fertilization of pig oocytes in vitro and on their subsequent fertilizing and developmental capacity in vitro. J Reprod Fertil 95:481-488 https://doi.org/10.1530/jrf.0.0950481
  39. Yoshida M (1993): Role of glutathione in the maturation and fertilization in pig oocytes in vitro. Mol Reprod Dev 35:76-81 https://doi.org/10.1002/mrd.1080350113