Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Expression of Human Lactoferrin Gene in Transgenic Rice (Oryza sativa L.)

  • Lee, Yong-Eok (Department of Biotechnology, Dongguk University) ;
  • Oh, Seong-Eun (College of Applied Life Science, Cheju National University) ;
  • Nishiguchi, Satoshi (College of Applied Life Science, Cheju National University) ;
  • Riu, Key-Zung (College of Applied Life Science, Cheju National University) ;
  • Song, In-Ja (College of Applied Life Science, Cheju National University) ;
  • Park, Shin-Young (Department of Clinical Pathology, Cheju Halla College) ;
  • Lee, Jin-Hyoung (Department of Biomechical Science, College of Natural Science, Hallym University) ;
  • Kim, Il-Gi (Department of Biomechical Science, College of Natural Science, Hallym University) ;
  • Suh, Suk-Chul (National Agricultural Science and Technology Institute, RDA) ;
  • Rhim, Seong-Lyul (Department of Biomechical Science, College of Natural Science, Hallym University) ;
  • Lim, Pyung-Ok (Department of Science Education, Cheju National University) ;
  • Lee, Hyo-Yeon (College of Applied Life Science, Cheju National University)
  • Published : 2007.06.30
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Abstract

Lactoferrin is an 80-kDa iron-binding glycoprotein known to exert many biological activities, such as facilitating iron absorption and having antimicrobial and anti-inflammatory effects. Rice can be a useful target for edible food plants to introduce human lactoferrin, because it has lower allergenicity and is likely to be safer than microorganisms or transgenic animals. A cDNA fragment encoding human lactoferrin (HLF) driven by the maize polyubiquitin promoter, along with herbicide resistance gene (bar) driven by CaMV 35S promoter, was introduced into rice (Oryza sativa L. cv. Dong Jin) using the Agrobacterium -mediated transformation system. Putative transformants were initially selected on the medium containing bialaphos. The stable integration of the bar and HLF genes into transgenic rice plants was further confirmed through polymerase chain reaction (PCR) and Southern blot analyses. The expression of the full length HLF protein from various tissues such as grains and young leaves of transgenic rice was verified by Western blot analysis. Analysis of progeny also demonstrated that introduced genes were stably inherited to the next generation at the Mendelian fashion.

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References

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