Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Isolation of Brucella spp from sere-positive native bulls and calves below twelve months old

브루셀라병 혈청검사 양성 수소와 12개월령 이하 소에서의 균 분리 및 동정

  • Ryu, Jae-Yun (Jechun branch, Chungbuk Livestock and Veterinary Research Institute) ;
  • Bun, Jung-Hyun (Jechun branch, Chungbuk Livestock and Veterinary Research Institute) ;
  • Lee, Hee-Young (Jechun branch, Chungbuk Livestock and Veterinary Research Institute) ;
  • Lee, Yong-Chang (Jechun branch, Chungbuk Livestock and Veterinary Research Institute) ;
  • Lee, Jong-Jin (Jechun branch, Chungbuk Livestock and Veterinary Research Institute) ;
  • Song, Young-Gak (Jechun branch, Chungbuk Livestock and Veterinary Research Institute) ;
  • Nam, Hyang-Mi (National Veterinary Research Quarantine Service)
  • 류재윤 (충청북도 축산위생연구소 제천지소) ;
  • 변정운 (충청북도 축산위생연구소 제천지소) ;
  • 이희영 (충청북도 축산위생연구소 제천지소) ;
  • 이용창 (충청북도 축산위생연구소 제천지소) ;
  • 이종진 (충청북도 축산위생연구소 제천지소) ;
  • 송영각 (충청북도 축산위생연구소 제천지소) ;
  • 남향미 (국립 수의과학검역원)
  • Published : 2007.09.30
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Abstract

This study was conducted to investigate the characteristics of brucellosis in Korean native cattle in a farm where bovine brucellosis was confirmed 3 times from September 2006 to March 2007. Of 74 bulls serum samples examined, 21 (28.4%) were positive by Rose-Bengal test (RBT) and Standard tube agglutination test (STAT). In the isolation test from seropositive bulls, B abortus was isolated and identified from 2 specimens (testis, intestinal lymph node) among 6 kinds of specimens including blood, urine, feces and soil. Isolation rate of intestinal lymph node and testis was 25% (3/12 cases) and 16.7% (2/12), respectively. B abortus was also isolated from calves below 12 months old, i.e., 1 isolate (25.0%) was confirmed from testis, 4 (40.0%) from supra-mammary lymph nodes and 1 (25.0%) from intestinal lymph node. All isolates had Brucella specific 16s r-RNA with 905-bp band detected by PCR assay. For the more effective control of bovine brucellosis in korea, this paper would like to suggest that all of bulls and calves should be included in the screening tests.

Keywords

References

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