Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Rapid diagnosis of bovine tuberculosis in slaughter cattle using PCR

PCR 기법을 이용한 도축 소의 결핵병 신속진단

  • Koh, Ba-Ra-Da (Gwangju Metropolitan Health & Environment Research Institute) ;
  • Kim, Hyun-Joong (Gwangju Metropolitan Health & Environment Research Institute) ;
  • Park, Duk-Woong (Gwangju Metropolitan Health & Environment Research Institute) ;
  • Park, Seong-Do (Gwangju Metropolitan Health & Environment Research Institute) ;
  • Kim, Jae-Ik (Gwangju Metropolitan Health & Environment Research Institute) ;
  • Park, Jong-Tae (Gwangju Metropolitan Health & Environment Research Institute) ;
  • Kim, Yong-Hwan (Gwangju Metropolitan Health & Environment Research Institute)
  • 고바라다 (광주광역시 보건환경연구원) ;
  • 김현중 (광주광역시 보건환경연구원) ;
  • 박덕웅 (광주광역시 보건환경연구원) ;
  • 박성도 (광주광역시 보건환경연구원) ;
  • 김재익 (광주광역시 보건환경연구원) ;
  • 박종태 (광주광역시 보건환경연구원) ;
  • 김용환 (광주광역시 보건환경연구원)
  • Published : 2007.09.30
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Abstract

Bovine tuberculosis is an important zoonosis worldwide. Mycobacterium bovis, the causative agent of this disease in cattle, is also a pathogen for humans and several economically important animals. The cases of tuberculosis are reported in two cow found at slaughter house located in Gwangju city. Histopathologically, in the lymph nodes, granulomas consisted of large areas of necrosis surrounded by variable thick bands of cellular infiltrate containing macrophages, Langhans-type multinucleated giant cells and lymphocytes. Lesions in the lung followed the same developmental pattern as did lesions in the lymph nodes with some exceptions. With the acid-fast staining, numerous mycobacteria were revealed in the lung and lymph nodes. M bovis was confirmed as a causative agent in these cattle using bacterial isolation and PCR and restriction fragment length polymorphism method based on a unique 12.7 kb fragment insertion sequence from the Mycobacterium tuberculosis genome and the pncA polymorphism, The insertion element IS6110 and IS1081 were present M bovis isolated from lungs and lymph nodes of cattle using PCR assay. These cases are interesting and important in public health aspect that M bovis-infected cattle were found during a routine post-mortem inspection at slaughter house.

Keywords

References

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