The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)
- Volume 20 Issue 3
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- Pages.1-12
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- 2007
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- 1229-4292(pISSN)
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- 2508-3619(eISSN)
The Effect of Houttuynia cordata thunberg(魚腥草) on the inhibition of growth of leiomyomas and apoptosis
어성초(魚腥草)가 자궁근종 세포의 성장억제와 세포자멸사에 미치는 영향
- Jeong, Byung-Chun (Dept. of OB&GY, Oriental Medicine, Daeguhaany University) ;
- Baek, Seung-Hee (Odri women's Traditional Korean Medical Clinic) ;
- Kim, Eun-Ha (Dept. of Original text and Medical history, Oriental Medicine, Daeguhaany University) ;
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Kim, Dong-Chul
(Dept. of OB&GY, Oriental Medicine, Daeguhaany University)
- Published : 2007.08.29
Abstract
Purpose: Uterine leiomyomas (fibroids) are common estrogen-dependent uterine tumors. Houttuynia cordata thunberg has cancer-preventing properties and often used in Chinese medicine. In the present study we used Houttuynia cordata thunberg to determine its effect on cell proliferation and apoptosis in human uterine leiomyoma cells. Methods: Primary cultured human uterine leiomyoma cells were treated with Houttuynia cordata thunberg. Cell viability analysis was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTS) assay and Flow cytometry was performed to ascertain the effects Houttuynia cordata thunberg. Expression of cell cycle related proteins and apoptosis related proteins were evaluated by Western blot analysis. Results: Cell viability was significantly influenced by Houttuynia cordata thunberg treatment in a dose-dependent manner in leiomyoma cells compare to normal myometrial cells. Flow cytometric analysis showed that Houttuynia cordata thunberg induced Sub G1 arrest. DNA fragmentation assay was carried out and apoptosis was detected. Activation of caspase-3, down-regulation of Bcl-2, with concomitant increase in p21 was observed. Houttuynia cordata thunberg treatment of uterine leiomyoma cellsresulted in a concentration-dependent cell death induced via the caspase dependent mechanism. Conclusion: These results suggest that Houttuynia cordata thunberg treatment in uterine leiomyoma cells leads to growth inhibition and induced apoptosis. These results suggest that Houttuynia cordata thunberg will be a promising agent for use in therapeutics agents against human uterine endometrial cancer.