The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)
- Volume 20 Issue 3
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- Pages.13-34
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- 2007
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- 1229-4292(pISSN)
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- 2508-3619(eISSN)
The Effect of Guibitang(歸脾湯) on the Ovarian Functions and Differential Gene Expression of Caspase-3, MAPK and MPG in Female Mice
귀비탕(歸脾湯)이 자성생쥐의 생식능력과 Caspase-3, MAPK, MPG유전자 발현에 미치는 영향
- Jung, Yeon-Chul (Dept. of OB&GY, Oriental Medicine, Daeguhaany University) ;
- Baek, Seung-Hee (Odri women's Traditional Korean Medical Clinic) ;
- Kim, Eun-Ha (Dept. of Original text and Medical history, Oriental Medicine, Daeguhaany University) ;
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Kim, Dong-Chul
(Dept. of OB&GY, Oriental Medicine, Daeguhaany University)
- Published : 2007.08.29
Abstract
Purpose: To evaluate the effect of administration of Guibitang on ovarian functions and differential gene expressions related cell viabilities caspase-3, MAPK and MPG in female mice. Methods: We administered the Guibitang to 6-week-old female ICR mice for 4, 8, or 12 days. Then, the female mice were injected PMSG and hCG for ovarian hyperstimulation. The mice divided into 3 different groups for each experiment. To compare the differences, we set a control group treated with plain water at the same volume by the same way. Results: administration of Guibitang, the mean number of total ovulated oocytes and the number of morphologically normal oocytes increased significantly compared to control group. And the rates of blastocyst formation from 2-cell stages alsa increased significantly compared to control group. Capase-3 gene expression which is known to maker gene for cell apoptosis were significantly lower than that of control group. And MAPK and MPG gene expressions for cell viability and DNA repair were same that of control group. Conclusion: From our results suggested that the medication of Guibitang has beneficial effect on reproductive functions of female mice via prevention of cell apoptosis and DNA damaging and promotion of cell proliferation.