Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Contamination and Detoxification of Aflatoxins

아플라톡신 오염 및 저감화 방안

  • Cho, So-Yean (Herbal Medicine Evaluation Team, Korea Food & Drug Administration) ;
  • Kang, In-Ho (Herbal Medicine Evaluation Team, Korea Food & Drug Administration) ;
  • Shim, Young-Hoon (Herbal Medicine Evaluation Team, Korea Food & Drug Administration) ;
  • Yang, Dong-Hyug (Herbal Medicine Evaluation Team, Korea Food & Drug Administration) ;
  • Oh, Seh-Wook (Herbal Medicine Evaluation Team, Korea Food & Drug Administration) ;
  • Lee, Byung-Hee (Herbal Medicine Evaluation Team, Korea Food & Drug Administration) ;
  • Hyeon, Seong-Ye (Herbal Medicine Evaluation Team, Korea Food & Drug Administration) ;
  • Chang, Seung-Yeup (Herbal Medicine Evaluation Team, Korea Food & Drug Administration) ;
  • Jeong, Choon-Sik (College of Pharmacy, Duksung Women's University) ;
  • Lee, Yong-Soo (College of Pharmacy, Duksung Women's University) ;
  • Kim, Young-Shik (Natural Products Research Institute, Seoul National University) ;
  • Kang, Shin-Jung (Herbal Medicine Evaluation Team, Korea Food & Drug Administration)
  • 조소연 (식품의약품안전청 의약품본부 생약평가부 한약평가팀) ;
  • 강인호 (식품의약품안전청 의약품본부 생약평가부 한약평가팀) ;
  • 심영훈 (식품의약품안전청 의약품본부 생약평가부 한약평가팀) ;
  • 양동혁 (식품의약품안전청 의약품본부 생약평가부 한약평가팀) ;
  • 오세욱 (식품의약품안전청 의약품본부 생약평가부 한약평가팀) ;
  • 이병희 (식품의약품안전청 의약품본부 생약평가부 한약평가팀) ;
  • 현성예 (식품의약품안전청 의약품본부 생약평가부 한약평가팀) ;
  • 장승엽 (식품의약품안전청 의약품본부 생약평가부 한약평가팀) ;
  • 정춘식 (덕성여자대학교 약학대학) ;
  • 이용수 (덕성여자대학교 약학대학) ;
  • 김영식 (서울대학교 약학대학) ;
  • 강신정 (식품의약품안전청 의약품본부 생약평가부 한약평가팀)
  • Published : 2007.09.30
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Abstract

South Korea is the representative consumption country of herbal medicines and most of herbal medicines circulating in Korea have been importing from the developing countries of Southeast Asia such as China, Vietnam, Indonesia and so forth. Domestic hygiene and safety are continuously proposed because herbal medicines which are circulating have the possibility could remain contaminants or residues. Physicochemical contaminants such as heavy metals, persistent organic pollutants, radionucleosides, microbial toxins, biological contaminants such as microorganisms and animals, agrochemical residues such as pesticides, substances used for fumigation, antiviral agents, and solvent residues are classified as major contaminants and residues in herbal medicines from 2005 September WHO.$^{1)}$ Currently our administration have established a permission standard and the inspection criteria against the heavy metal, the residual pesticides and a residual sulfur dioxide. Furthermore our administration is continuously monitoring and conducting researches for the policies and their scientific ground against herbal medicines. But the appearances or discoveries of the harmful new species due to environmental and industrial developments are becoming social problems. Therefore it may be necessary to continuously consider and investigate regarding hereupon. Recently, the contamination of the mycotoxins against foods such as cereals, nuts and the powdered red pepper have developed and started became problematic issue, and possibility of contamination against the herbal medicine is proposed. And since populations who are using the herbal medicines very limited to several nations, recognition and researches about contamination of mycotoxins in herbal medicines are very insufficient. Therefore it will be need to more focus on the international regulation of quality control and safety for herbal medicines. Now on, we are going to introduce the importance, occurrence, characteristic properties, World-wide research trends and detoxification of aflatoxins, which is known as the most potent mutagen, carcinogen and teratogen mycotoxins.

Keywords

References

  1. Quality control methods for medicinal plant materials, World Health Organization draft, (2005)
  2. Sargeant, K., Sheridan, A., O'Kelly, J. and Carnaghan, R. B. A. (1961) Toxicity associated certain samples of groundnuts. Nature 192: 1095-1097 https://doi.org/10.1038/1921095a0
  3. International Agency for Research on Cancer, (1993) IARC Monographs on the Evaluation of Carcinogenic Risk to Humans : Heterocyclic Amines and Mycotoxins. World Health Organisation and International Agency for Research on Cancer, Lyon, Vol. 56, 245-395, IARC, Lyon France
  4. Roy, A. K., Sinha, K. K. and Chourasia, H. K. (1988) Aflatoxin contamination of some common drug plants. Appl. Environ. Microbiol. 54: 842-843
  5. Reif, K. and Metzger, W. (1995) Determination of aflatoxins in medicinal herbs and plant extracts. J. Chromatgr. A. 692: 131-136 https://doi.org/10.1016/0021-9673(94)00841-V
  6. Ventura, M., Gomez, A., Anaya, I., Diaz, J., Broto, F., Agut, M. and Comellas, L. (2004) Determination of aflatoxins $B_1,\;G_1,\;B_2\;and\;G_2$ in medicinal herbs by liquid chromatography tandem mass spectrometry. J. Chromatgr. A. 1048: 25-29
  7. D'Mello, J. P. F. and Macdonald, A. M. C. (1997) Mycotoxins. Animal Feed Sci. Technol. 69: 155-166 https://doi.org/10.1016/S0377-8401(97)81630-6
  8. van Egmond, H. P. (1989) Aflatoxin M1 : occurrence, toxicity, regulation. In van Egmond, H. P. (Ed.), Mycotoxins in diary products. Elservir Applied Science, London, 11-55
  9. Santin E, (2005) Mould growth and mycotoxin production, In: Diaz D. E. (Ed.). The mycotoxin blue book, Nottingham University Press, Nottingham, UK, 225-234
  10. Smith J. E. and Moss, M. O. (1985) Mycotoxins : Formation, Analysis and Significance. John Wiley and Sons, Chichester, 36-41
  11. Yoshizawa, T. (1991) Natural occurrence of mycotoxins in small grain cereals(wheat, barley, rye, oats, sorghum, millet, rice). In: Smith, J. E., Henderson, R. S.(Eds.), Mycotoxins and Animal Foods. CRC Press, Boca Raton, FL, 301-324
  12. van Egmond, H. P. (2004) Natural toxins : risk, regulations and analytical situation in Europe. Anal. Bioanal. Chem. 378: 1152-1160 https://doi.org/10.1007/s00216-003-2373-4
  13. Shotwell, O. L. (1991) Natural occurrence of mycotoxins in corn. In: In: Smith, J. E., Henderson, R. S.(Eds.), Mycotoxins and Animal Foods. CRC Press, Boca Raton, FL, 325-340
  14. Strange, R. N. (1991) Natural occurrence of mycotoxins in groundnuts, cottonseed, soya and cassava. In: In: Smith, J. E., Henderson, R. S.(Eds.), Mycotoxins and Animal Foods. CRC Press, Boca Raton, FL, 341-362
  15. Peers, F. G. and Linsell, C. A. (1973) Dietary aflatoxins and liver cancer- A population based study in Kenya. Br. J. Cancer. 27: 473-484 https://doi.org/10.1038/bjc.1973.60
  16. Shank, R. C., Wogan, G. N., Gibson, J. B. and Nondasuta, A, (1972) Dietary aflatoxins and human liver cancerII. Aflatoxins in market foods and foodstuffs of Thiland and Hong Kong. Food Cosmet. Toxicol. 10: 61-69 https://doi.org/10.1016/S0015-6264(72)80047-6
  17. 서정운 (1991) 미생물 독소, 형설출판사
  18. Council for Agricultural Science and Technology, 2003. Mycotoxins: Risks in Plant, Animal, and Human Systems. Task Force Report No. 139. Ames, Iowa
  19. Robens, J. F. and Richard, J. L. (1992) Aflatoxins in animal and human health. Rev. Environ. Contam. Toxicol. 127: 69- 94
  20. Kirk, G. D., Bah, E. and Montesano, R. (2006) Molecular epidemiology of human liver cancer: insights into etiology, pathogenesis and prevention from The Gambia, West Africa. Carcinogenesis 27: 2070-2082 https://doi.org/10.1093/carcin/bgl060
  21. Adhikari, M., Ramjee, G and Berjak, P. (1994) Aflatoxin, kwashiorkor, and morbidity. Nat. Toxins 2: 1-3 https://doi.org/10.1002/nt.2620020102
  22. Palmgren, M. S. and Hayes, A. W. (1987) Aflatoxins in foods, in Mycotoxins in Food, ed. P. Krogh., Academic Press, London, pp.65-96
  23. 하덕모 (1995) 최신식품미생물학, 신광출판사
  24. Brown, R. L., Cleveland, T. E., Payne, G. A., Woloshuk, C. P., Cambel, K. W. and White, D. G. (1995) Determination of resistance to aflatoxin production in maize kernels and detection of fungal colonization using an Aspergillus flavus transformant expressing Escherchia coli $\beta$-glucuronidase. Phytopathology 85: 983-989 https://doi.org/10.1094/Phyto-85-983
  25. Saleemullah, A. I., Iqtidar, A. and Hamidullah S. (2006) Aflatoxin contents of stored and artificially inoculated cereals and nuts, Food Chem. 98: 699-703 https://doi.org/10.1016/j.foodchem.2005.06.034
  26. Lewis, L., Onsongo, M., Njapau, H., Schurz-Rogers, H., Luber, G., Kieszak, S., Nyamongo, J., Baker, L., Dahiye, A., Misore, A., DeCock, K. and Rubin, C. (2005) Aflatoxin contamination of commercial maize products during an outbreak of acute aflatoxicosis in Eastern and Central Kenya. Environ. Health Persp. 113: 1762-1767
  27. Diener, U. L., Pettit, R. E. and Cole, R. J. (1982) Aflatoxins and other mycotoxins in peanuts. In Peanut Science and Technology, eds H.E. Pattee and C.T. Young. American Peanut Research and Education Society, Yoakum, TX, 486-519
  28. Brackett, R. E. (1989) Strategies for dealing with aflatoxins in peanuts. In trend in Food Product Development, eds Yam, T. C. and Tan. C. Singapore, 83-91
  29. Ruker, K. S., Kvien, C. K., Calhoun, K., Henning, R. J., Koeler, P. E., Ghate, S. R., and Holbrook, C. C. (1994) Sorting peanuts by pod density to improve quality and kernel maturity distribution and to reduce aflatoxin. Peanut Sci. 21: 147-152 https://doi.org/10.3146/i0095-3679-21-2-17
  30. Manabe, M., Tsuruta, O., Goto, T. and Matsuura, S. (1978) Study on distribution of mycotoxin-producing fungy : (part 4) Mycotoxin-producing ability of Aspergillus strains inhabited in Southeast Asia. Rept. Natl. Food Res. Inst. 33: 49-56
  31. Cotty, P. J. (1991) Effect of harvest date on aflatoxin contamination of cottonseed. Plant Disease 75: 312-314 https://doi.org/10.1094/PD-75-0312
  32. Lewis, L., Onsongo, M., Njapau, H., Schurz-Rogers, H., Luber, G., Kieszak, S., Nyamongo, J., Baker, L., Dahiye, A., Misore, A., DeCock, K. and Rubin, C. (2005) Aflatoxin contamination of commercial maize products during an outbreak of acute aflatoxicosis in Eastern and Central Kenya. Environ. Health Persp. 113: 1762-1767
  33. Sanders, T. H., Blankenship, P. D., Cole, R. J. and Hill, R .A. (1984) Effects of soil temperature and drought on peanut pod and stem temperatures relative to Aspergillus flavus invasion and aflatoxin contamination. Mycopathologia 86: 51-54 https://doi.org/10.1007/BF00437229
  34. D'Mello, J. P. E., Macdonald, A. M. C. and Cochrane, M. P. (1993) A preliminary study of the potential for mycotoxin production in barley grain. Aspects Appl. Biol. 36: 375-382
  35. Porter, D. M., Wright, F. S. and Steele, J. L. (1986) Relationship to microscopic shell damage to colonization of peanut by Aspergillus flavus. Oleagneux. 41: 23-28
  36. Chiou, R. Y. Y., Koehler, P. E. and Beuchat, L. R. (1984) Hygroscopic characteristics of penut components and their influence on growth and aflatoxin production by Aspergillus parasiticus. J. Food Protect. 47: 791-794 https://doi.org/10.4315/0362-028X-47.10.791
  37. Viquez, O. M., Castell Perez, M. E., Shelby, R. A. and Brown, G. (1994) Aflatoxin contamination in corn samples due to environmental conditions, aflatoxin-producing strains, and nutrients in grain grown in Costa Rica. J. Agric. Food Chem. 42: 2551-2555 https://doi.org/10.1021/jf00047a033
  38. Diener, U. L., Pettit, R. E. and Cole, R. J. (1982) Aflatoxins and other mycotoxins in peanuts. In Peanut Science and Technology, eds H.E. Pattee and C.T. Young. American Peanut Research and Education Society, Yoakum, TX, 486-519
  39. Sanders, T. H., Cole, R. J., Blankenship, P. D., and Dorner, J. W. (1993) Aflatoxin contamination of peanuts from peanuts drought stressed in pod or root zones. Peanut Sci. 20: 5- 8 https://doi.org/10.3146/i0095-3679-20-1-2
  40. Badii, F. and Moss, M. O. (1988) The effect of the fungicides tridemorph, fenpropimorph and fenarimol on growth and aflatoxin production by Aspergillus parasiticus Speare. Lett. Appl. Microbiol. 7: 37-39 https://doi.org/10.1111/j.1472-765X.1988.tb01247.x
  41. Rustom, I. Y. S., Lopez-Leiva, M. H. and Nair, B. M. (1993) Effect of pH and heat treatment on the mutagenic activity of peanut beverage contaminated with aflatoxin B1. Food Chem. 46: 37-42 https://doi.org/10.1016/0308-8146(93)90072-N
  42. Park, D. L. and Liang, B. (1993) Perspectives on aflatoxin control for human food and animal feed. Trend Food Sci. Technol. 4: 334-342 https://doi.org/10.1016/0924-2244(93)90104-I
  43. Albert, J. F., Engelbrechut, Y., Steyn, P. S., Holzapfel, W. H. and van Zyl, W. H. (2006) Biological degradation of aflatoxin B1 by Rhodococcus erythropolis cultures. Int. J. Food Microbiol. 109: 121-126 https://doi.org/10.1016/j.ijfoodmicro.2006.01.019
  44. Hormisch, D., Brost, I., Kohring, G. W., Giffhorn, F., Krippenstedt, R. M., Stackbrandt, E., Faber, P. and Holtzapfel, W. H. (2004) Mycobacterium fluoranthenivorants sp. nov., a fluoranthene and aflatoxin $B_1$ degrading bacterium from contaminated soil of a former coal gas plant. Syst. Appl. Microbiol. 27: 553-660
  45. Sakai, M., Miyauchi, K., Kato, N., Massai, E. and Fukuda, M. (2003) 2-Hydroxypenta-2,4-dienoate metabolic pathway genes in a strong polychlorinated biphenyl degrader, Rhodococcus sp. strain RHA1. Appl. Environ. Microbiol. 69: 327- 433 https://doi.org/10.1128/AEM.69.1.327-333.2003
  46. Albert, J. F., Engelbrechut, Y., Steyn, P. S., Holzapfel, W. H., van Zyl, W. H. (2006) Biological degradation of aflatoxin B1 by Rhodococcus erythropolis cultures. Int. J. Food Microbiol. 109: 121-126 https://doi.org/10.1016/j.ijfoodmicro.2006.01.019
  47. Yamada, A., Kishi, H., Sugihama, K., Hatt, T., Nakamura, K., Massai, E. and Fukuda, M. (1998) Two nearly identical aromatic compound hydrolase genes in a strong polychlorinated biphenyl degrader, Rhodococcus sp. strain RHA1. Appl. Environ. Microbiol. 64: 2006-2012
  48. Shetty, P. H., Hald, B. and Jespersen, L. (2007) Surface binding of aflatoxin $B_1$ by Saccharomyces cerevisiae strains with potential decontaminating abilities in indigenous fermented foods. Int. J. Food Microbiol. 113: 41-46 https://doi.org/10.1016/j.ijfoodmicro.2006.07.013
  49. Shetty, P. H. and Jespersen, L. (2006) Saccharomyces cerevisiae and lactic acid bacteria as potential mycotoxin decontaminating agents. Trends Food Sci. Technol. 17: 48-55 https://doi.org/10.1016/j.tifs.2005.10.004
  50. Aguilar-Uscanga, B. and Francois, J. M. (2003) A study of the yeast cell wall composition and structure in response to growth conditions and mode of cultivation. Lett. Appl. Microbiol. 37: 268-274 https://doi.org/10.1046/j.1472-765X.2003.01394.x
  51. Zhang, X. B. and Ohta, Y. (1991) Binding of mutagens by fractions of lactic acid bacteria on mutagens the cell wall skeleton. J. Dairy Sci. 74: 1477-1481 https://doi.org/10.3168/jds.S0022-0302(91)78306-9
  52. Celyk, K., Denly, M. and Savas, T. (2003) Reduction of toxic effects of aflatoxin by using baker yeast (Saccharomyces cerevisiae) in growing broiler chicken diets. Revista Brasileira de Zootecnia. 32: 615-619 https://doi.org/10.1590/S1516-35982003000300013
  53. Brekke, O. L., Stringfellow, A. C. and Peplinski, A. C. (1978) Aflatoxin inactivation in corn by ammonia gas : laboratory trials. J. Agric. Food. Chem. 26: 1383-1389 https://doi.org/10.1021/jf60220a050
  54. Mckenzie, K. S., Sarr, A. B., Mayura, K., Bailey, R. H., Miller, D. R., Rogers, T. D., Norred, W. P., Voss, K. A., Plattner, R. D., Kubena, L. F. and Pillips T. D. (1997) Oxidative degradation and Detoxification of mycotoxins using a novel source of ozon. Food Chem. Toxicol. 35: 807-820 https://doi.org/10.1016/S0278-6915(97)00052-5
  55. Dollear, F. G., Mann, G. E., Codifier, L. P., Gardner, H. K., Koltun, S. P. and Vix, H. L. E. (1968) Elimination of aflatoxins from peanut meal. J. Am. Oil Chem. Soc. 45: 862-865 https://doi.org/10.1007/BF02540171
  56. Suzuki, T., Noro, T., Kawamura, Y., Fukunaga, K., Watanabe, M., Ohta, M., Sugiue, H., Sato, Y., Kohno, M. and Hotta, K. (2002) Decontamination of aflatoxin-forming fungus and elimination of aflatoxin mutagenicity with electrolyzed NaCl anode solution. J. Agric. Food Chem. 50: 633- 641 https://doi.org/10.1021/jf0108361
  57. Mendez-Albores, A., Del Rio-Garcia, J. C. and Moreno- Martinez, E. (2007). Decontamination of aflatoxin duckling feed with aqueous citric acid treatment. Animal Feed Sci. Technol. 135: 249-262 https://doi.org/10.1016/j.anifeedsci.2006.07.009
  58. Proctor, A. D., Ahmedna, M., Kumar, J. V. and Goktepe, I. (2004) Degradation of aflatoxins in peanut kernels/flour by gaseous ozonization and mild heat treatment. Food Addit. Contam. 21: 786-793 https://doi.org/10.1080/02652030410001713898
  59. Maeba, H., Takamoto, Y., Kaminura, M. and Miura, T. (1988) Destruction and detoxification of aflatoxins with ozone. J. Food Sci. 53: 667-668 https://doi.org/10.1111/j.1365-2621.1988.tb07786.x
  60. Inan, F., Pala, M. and Doymaz, I. (2007) Use of ozone in detoxification of aflatoxin $B_1$ in red pepper. J. Stored Prod. Res. 43: 425-429 https://doi.org/10.1016/j.jspr.2006.11.004
  61. Park, D. L. (1993) Perspectives on mycotoxin decontamination procedures. Food Addit. Contam. 10: 49-60 https://doi.org/10.1080/02652039309374129
  62. Bauer, J. (1994) Moglichkeiten zur Entgiftung mycotoxinhaltier Futtermittel. Monatsh. Veterinarmed 49: 175-181
  63. Huwig, A., Fremund, S., Kappeli, O. and Dutler, H. (2001) Toxicol. Lett. 122: 179-188 https://doi.org/10.1016/S0378-4274(01)00360-5
  64. Babich, H. and Stotzky, G. (1980) Gaseous and heavy metal pollutants. In : Burns, R.G., Slater, J.H., (Eds.), Experimental Microbial Ecology. Blackwell, Oxford, p.631
  65. Masimango, N., Remacle, J. and Ramaut, J. L.(1978) The reole of adsorption in the elimination of aflatoxin B1 from contaminated media. Eur. J. Appl. Microbiol. Biotechnol. 6: 101-105 https://doi.org/10.1007/BF00500861
  66. Phillips, T. D., Kubena, L. F., Harvey, R. B., Taylor, D.S. and Heidelbaugh, N. D. (1988) Hydrated sodium calcium alominosilicate: a high affinity sorbent for aflatoxin. Poult. Sci. 67: 243-247 https://doi.org/10.3382/ps.0670243
  67. Kamimura, H. (1989) Removal of mycotoxins during food processing. In Mycotoxins and phycotoxins, eds S. Natori, K. Hashimoto and Y. Ueno. Elsevier Scinece Publishers, Amsterdam, 169-176
  68. Mann, G. E., Codifer, L. P. and Dollear, F. G. (1967) Effect of heat on aflatoxin in oilseed meals. J. Agric. Food Sci. 15: 1090-1092 https://doi.org/10.1021/jf60154a013
  69. Coomes, T. J., Crowther, P. C., Feuell, A. J. and Francis, B. J. (1966) Experimental detoxification of groundnut meals containing aflatoxin. Nature. 209: 406-407 https://doi.org/10.1038/209406a0
  70. Farah, Z., Martins, M. J. R. and Bachmann, M. R. (1983) Removal of aflatoxin in raw unshelled peanuts by a traditional salt boiling process practiced in Northeast of Brazil. Lebensm. Wise. Technol. 16: 122-124
  71. Staron, T., Thirouin, D., Perrin, L. and Frere, G. (1980) Microwave treatment of biological food product. Ind. Aliment. Agric. 12: 1305-1312
  72. Luter, L., Wyslouzil, W. and Kashyap, S. C. (1982) The destruction of aflatoxins in peanuts by microwave roasting. Can. Inst. Food Sci. Technol. J. 15: 236-238 https://doi.org/10.1016/S0315-5463(82)72545-3
  73. Pluyer, H. R., Ahmed, E. M. and Wei, C. I. (1987) Destruction of aflatoxin on peanuts by oven-and microwave-roasting. J. Food Protect. 50: 504-508 https://doi.org/10.4315/0362-028X-50.6.504
  74. Diehl, J. F. (1990). Safety of Irradiated Foods, ed. J. F. Diehl. Marvel Dekker, New York, 397-444
  75. Samarajeewa, U., Sen, A. C., Cohen, M. D. and Wei, C. T. (1990) Detoxification of aflatoxins in foods and feeds by physical and chemical methods. J. Food Protect. 53: 489- 501 https://doi.org/10.4315/0362-028X-53.6.489
  76. Andrellos, P. J., Beckwith, A. E. and Epply, R. M. (1967) Photochemical changes of aflatoxin B1. J. Assoc. Off. Anal. Chem. 50: 346-350
  77. Altug, T., Yousef, A. E., and Marth, E. H. (1990) Degradation of aflatoxin B1 in dried figs by sodium bisulfite with or without heat, ultraviolet energy or hydrogen peroxide. J. Food. Protect. 53: 581-582 https://doi.org/10.4315/0362-028X-53.7.581
  78. Park, B. J., Takatori, K., Sugita-Konishi, Y., Kim, I. H., Lee, M. H. Han, D. W., Chung, K. H., Hyun, S. O., Park, J. C. (2007) Degradation of mycotoxins using micro-induced argon plasma at atmospheric pressure, Surface and Coatin. Technol. 201: 5733-5737 https://doi.org/10.1016/j.surfcoat.2006.07.092
  79. Temcharoen, P. and Thilly, W. G.(1982) Removal of aflatoxin $B_1$ toxicity but not mutagenicity by 1 megarad gamma radiation of peanut meal. J. Food Safety, 4: 199-205 https://doi.org/10.1111/j.1745-4565.1982.tb00445.x
  80. Chiou, R. Y. Y., Lin, C. M. and Shyu, S. L. (1990) Property characterization of peanut kernels subjected to gamma irradiation and its effect on the outgrowth and aflatoxin production by asperfillus parasiticus. J. Food Sci. 55: 210-213 https://doi.org/10.1111/j.1365-2621.1990.tb06054.x
  81. Shanta, T. and Murthy, V. S. (1977) Photo-destruction of aflatoxin in groundnut oil. Indian J. Technol. 15: 453-454
  82. Koseki, P. M., Villlavicencio, A. L. C. H., Brito, M. S., Nahme, L. C., Sebastiao, K. I., Rela, P. R., Almeida-Muradian, L. B., Mancini-Filho, J., Freitas, P. C. D. (2002) Effects of irradiation in medicinal and eatable herbs. Radiat. Phys. Chem. 63: 681-684 https://doi.org/10.1016/S0969-806X(01)00658-2