Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Quantitative Determination on the Constituents of the Stem Bark and the Leaf Shoot of Kalopanax pictus by HPLC Analysis

HPLC 분석에 의한 해동피와 개두릅의 성분함량 비교

  • 김민영 (상지대학교 친환경식물학부) ;
  • 유영민 (상지대학교 친환경식물학부) ;
  • 남정환 (농촌진흥청 고령지농업연구소) ;
  • 최종원 (경성대학교 약학대학) ;
  • 박희준 (상지대학교 친환경식물학부)
  • Published : 2007.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

To evaluate the leaf shoots and stem barks of Kalopanax pictus (Araliaceae) as an edible vegetable and a traditional medicinal drug, respectively, the compounds of syringin, liriodendrin, astragalin, quercetin, and kalopanaxsaponins were quantitatively measured by HPLC analysis. The leaf shoot exhibited low contents of syringin, liriodendrin and kalopanaxsaponins but a high chlorogenic acid content, whereas the grown leaves contained very high amounts of kalopananxsaponins. In contrast, the stem bark had very high amounts of syringin and liriodendrin and relatively low kalopanaxsaponins. In particular, the kalopanaxsaponin contents were rapidly increased with monthly variation until October but decreased from September. It was also observed that the leaf shoot contained chlorogenic acid by 30.73 mg/g and the barks showed the concentration of liriodendrin by 20.75 mg/g. These results indicate that high contents of syringin and liriodendrin in the stem bark and high contents of chlorogenic acid in the leaf shoot support scientific bases on the traditional uses of K. pictus as a medicinal drug and a functional food, respectively.

Keywords

References

  1. 지형준, 이상인 (1988) 대한약전외 한약(생약) 규격집 주해서, 407. 한국메디칼인덱스사, 서울
  2. Sano, K., Sanada, S., Ida, Y. and Shoji, J. (1991) Sudies on the constituents of the stem bark of Kalopanax pictus Nakai. Chem. Pharm. Bull. 39: 865-870 https://doi.org/10.1248/cpb.39.865
  3. Shao, C. J., Nakai, R., Ohtani, K., Xu, J. D. and Tanaka, O. (1989) Saponins from leaves of Kalopanax septemlobus (Thunb.) Koidz, Structures of kalopanaxsaponins La, Lb and Lc. Chem. Pharm. Bull. 37: 3251-3252 https://doi.org/10.1248/cpb.37.3251
  4. Choi, J. W., Huh, K., Kim, S. H., Lee, K. T., Park, H. J. and Han, Y. N. (2002) Antinociceptive and anti-rheumatoidal effects of Kalopanax pictus extract and its saponin components in experimental animals, J. Ethnopharmacol. 79: 199-204 https://doi.org/10.1016/S0378-8741(01)00383-X
  5. Lee, K. T., Sohn, I. C., Park, H. J., Kim, D. W., Jung, G. O. and Park, K. Y. (2002) Essential moiety for antimutagenic and cytotoxic activity of hederagenin monodesmosides isolated from the stem Bark of Kalopanax pictus, Planta Med. 66: 329-332 https://doi.org/10.1055/s-2000-8539
  6. Park, H. J., Kwon, S. H., Lee, J. H., Lee, K. H., Miyamoto, K. and Lee K. T. (2001) Kalopanaxsaponin A is a basic saponin structure for the anti-tumor activity of hederagenin monodesmosides, Planta Med. 67: 118-121 https://doi.org/10.1055/s-2001-11516
  7. Nam, J. H., Jung H. J., Choi, J. W., Park, K. K., Kim, W. B., Lee, M. S. and Park, H. J. (2006) Monthly variation of the kalopanaxsaponin content in the leaves of Kalopanax pictus, Kor. J. Pharmacogn. 37: 184-189
  8. Kim, D. H., Yu, K. W., Bae, E. A., Park H, J. and Choi, J. W. (1998) Metabolism of Kaopanaxsaponin B and H by Human Intestinal Bacteria and Antidiabetic Activity of Their Metabolites, Biol. Pharm. Bull. 21: 360-365 https://doi.org/10.1248/bpb.21.360
  9. Kim, D. H., Kim, E. A., Han, M. J., Park, H. J. and Choi, J. W. (2002) Metabolism of kalopanaxsaponin K by human intestinal bacteria and antitheumatoidal arthritis activity of their metabolites, Biological and Pharmaceutical Bulletin 25: 68-71 https://doi.org/10.1248/bpb.25.68
  10. Li, X. C., Barnes, D. L. and Khan, I. A. (2001) A new lignan glycoside from Eleutherococcus senticosus, Planta Med. 67: 776-778 https://doi.org/10.1055/s-2001-18352
  11. Jung, H. J., Park, H. J., Kim, R. G., Shin, K. M., Ha, J., Choi, J. W., Kim, H. J. and Lee, K. T. (2003) In vivo antiinflammtory and antinociceptive effects of Liriodendrin isolated from the stem bark of Acanthopanax senticosus, Planta Med. 69: 610-616 https://doi.org/10.1055/s-2003-41127
  12. Choi, J., Shin, K. M., Park, H. J., Jung, H. J., Ha, J., Kim, H. J., Lee, Y. S. and Lee, K. T. (2004) Anti-inflammatory and antinociceptive effects of sinapyl alcohol and its glucoside syringin, Planta Med. 70: 1027-1032 https://doi.org/10.1055/s-2004-832642
  13. Nakatani N, Kayano S, Kikuzaki H, Sumino K, Katagiri K and Mitani T. (2000) Quantitative determination, and antioxidative activities of chlorogenic acid isomers in prune (Prunus domestica L.), J. Agric. Food Chem. 48: 5512-5516 https://doi.org/10.1021/jf000422s
  14. Auger. C., Laurent, N., Laurent, C., Besacon, P., Caporiccio, B., Teissedre, P. L. and Rouanet, J. M. (2004) Hydroxycinnamic acid do not prevent aortic atherosclerosis in hypercholesterolemic golden Syrian hamsters, Life Sci. 74: 2365- 2377 https://doi.org/10.1016/j.lfs.2003.09.062