Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Quality Evaluation by the Addition of Pine Needle and Artemisia princeps Extracts in Vinegared Kochujang

솔잎, 약쑥추출물을 첨가한 초고추장의 품질평가

  • Published : 2007.09.30
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Abstract

This study was carried out to develop natural vinegared kochujang. We first formulated vinegared kochujang containing pine needle (PN) and Artemisia princeps (AP) extracts. A dominant strain was isolated from the vinegared kochujang and identified as Bacillus amyloliquefaciens ER282 based on the 16S rDNA sequence. The plant extracts of PN and AP were also investigated for their antibacterial activity and showed enough good activities to use for vinegared kochujang as a natural preservative. Quality characteristics of vinegared kochujang were evaluated, based on total cell numbers, pH, acidity, sugar content, color and sensory evaluation during storage at $25^{\circ}C$ and $37^{\circ}C$, respectively, for 3 weeks. No significant differences were found in the pHs and acidities of vinegared kochujang added with the plant extracts of PN and AP during their storage compared to starting time; however, total cell numbers were gradually decreased during the storage at $25^{\circ}C$ and $37^{\circ}C$, as the storage period was increased. For sensory evaluation, overall preference continuously declined depending on storage period, but the addition of 3% extracts of PN could delay the quality loss of vinegared kochujang during storage at $25^{\circ}C$ and $37^{\circ}C$ for 3 weeks.

솔잎, 약쑥추출물을 첨가하여 제조한 초고추장을 3주 동안 $25^{\circ}C$, $37^{\circ}C$에 저장하면서 각각 2일, 1일 간격으로 분석 후 초고추장의 품질을 검토하였다. 우선, 초고추장에서 우점균주가 Bacillus amyloliquefaciens ER282 라는 것을 분리${\cdot}$동정 하였고 이에 대하여 솔잎, 약쑥추출물의 1, 3, 5, 10% 농도로 항균력 실험을 한 결과 대부분의 추출물에서 효과를 나타내었다. 초고추장 저장중의 총균수 변화는 저장온도와 관계없이 $25^{\circ}C$와 $37^{\circ}C$ 모두 무첨가구에 비해 현저히 감소하였다. pH와 산도의 변화는 $25^{\circ}C$, $37^{\circ}C$에서 무첨가구와 저장 기간에 따른 뚜렷한 차이가 없었다. 당도는 $25^{\circ}C$에 저장한 무첨가구가 초기 $67^{\circ}$Brix, 21일째 $77^{\circ}$Brix를 주정 3% 첨가구는 초기 $63^{\circ}$Brix, 21일째 $74^{\circ}$Brix로 나타났으며 추출물 첨가구는 초기 $61{\sim}74^{\circ}$Brix, 21일째 $67{\sim}78^{\circ}$Brix의 변화를 보여 약간 증가하는 양상을 보였다. $37^{\circ}C$에 저장한 무첨가구는 초기 $67^{\circ}$Brix, 21일째 $83^{\circ}$Brix를 주정 3% 첨가구는 초기 $63^{\circ}$Brix, 21일째 $73^{\circ}$Brix를 보였으며 추출물 첨가구는 $61{\sim}74^{\circ}$Brix, 21일째 $73{\sim}85^{\circ}$Brix로 나타나 $25^{\circ}C$와 마찬가지로 증가하였다. 색도는 솔잎, 약쑥추출물 대부분이 첨가량이 증가함에 따라 명도값은 증가하였으나 적색과 황색값은 크게 감소하였고 무첨가구와 주정 3%첨가구도 같은 경향이었다. 그러나 $25^{\circ}C$에서 주정 1%와 약쑥추출물 1% 첨가구가 21일이 되어도 저장 초기값과 비슷한 값을 나타내었고, $37^{\circ}C$에서는 약쑥추출물 1%, 약쑥추출물 3% 첨가구가 저장 초기값과 큰 차이를 보이지 않았다. 관능검사 결과 추출물 첨가구가 무첨가구 및 주정 3% 첨가구보다 기호도가 높은 편이였으나 저장기간에 따라 전체적인 기호도는 감소하는 경향이었다. 그러나 저장온도에 관계없이 솔잎추출물 3% 첨가구가 전체적인 기호도면에서 유의적으로 높은 기호도를 나타내었다.

Keywords

References

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